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Preparation method and application of ready-to-use antibody reagent (immunohistochemical)

A preparation method and immunohistochemical technology, applied in the direction of biological testing, material inspection products, etc., can solve the problems of antibodies (protein loss of efficacy, short validity period, unstable antibody reagents, etc., achieve good staining effect, improve stability, antibody good stability effect

Pending Publication Date: 2022-08-02
杭州迪英加科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Based on the problems that the above-mentioned existing antibody diluents for preparing antibody reagents are unstable, easily lose efficacy of antibodies (proteins), and have a short validity period, the present invention provides a preparation method and application of ready-to-use antibody reagents (immunohistochemistry), To make the preparation of antibody reagents unstable for the above-mentioned existing antibody diluent, easily make the antibody (protein) lose its efficacy, and the technical problems of short validity period propose the following technical solutions:

Method used

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  • Preparation method and application of ready-to-use antibody reagent (immunohistochemical)
  • Preparation method and application of ready-to-use antibody reagent (immunohistochemical)
  • Preparation method and application of ready-to-use antibody reagent (immunohistochemical)

Examples

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Effect test

Embodiment 1

[0074] A preparation method and application of a ready-to-use antibody reagent (immunohistochemistry), the general-purpose antibody diluent, and the preparation of raw materials are composed of:

[0075] Biological buffer:

[0076]

[0077] Active ingredients:

[0078]

[0079] The preparation process is as follows:

[0080] Preparation operation step 1: prepare biological buffer (phosphate buffer, pH7.4, 1000ml), weigh 1.44gNa 2 HPO 4 , 0.24g KH 2 PO 4 , 8g NaCl, 0.2g KCl, dissolved in 1000ml of distilled water, after fully dissolved, adjust the pH value to 7.4.

[0081]Preparation operation step 2: Measure 100ml of biological buffer, weigh 0.1g of gelatin and 0.05g of case egg, and dissolve in a water bath at 60-80°C, which is S1 solution.

[0082] Preparation operation step 3: Weigh 0.5g of dextran, 0.05g of polyvinylpyrrolidone, 10g of sucrose, 0.5g of trehalose, 0.5g of BSA, and 2g of polyethylene glycol into the S1 solution, fully stirring and dissolving, tha...

Embodiment 2

[0085] A preparation method and application of a ready-to-use antibody reagent (immunohistochemistry), the general-purpose antibody diluent, the preparation of raw materials is composed of:

[0086] Biological buffer:

[0087]

[0088] Active ingredients:

[0089] dextran 1.5% Polyvinylpyrrolidone 0.5% sucrose 5% trehalose 0.5% BSA 0.5% gelatin 0.1% casein 0.01% polyethylene glycol 1.5% Tween-20 0.05% protease inhibitor complex 0.5% Proclin300 0.05%

[0090] The preparation process is as follows:

[0091] Preparation operation step 1: prepare biological buffer (phosphate buffer, pH7.4, 1000ml), weigh 1.44gNa 2 HPO 4、 0.24g KH 2 PO 4 , 8g NaCl, 0.2g KCl, dissolved in 1000ml of distilled water, after fully dissolved, adjust the pH value to 7.4.

[0092] Preparation operation step 2: Measure 100 ml of biological buffer, weigh 0.1 g of gelatin, 0.01 g of case egg, and dissolve in a water bath at 60...

Embodiment 3

[0096] A preparation method and application of a ready-to-use antibody reagent (immunohistochemistry), the general-purpose antibody diluent, the preparation of raw materials is composed of:

[0097] Biological buffer:

[0098]

[0099] Active ingredients:

[0100] dextran 1.0% Polyvinylpyrrolidone 0.15% sucrose 3% trehalose 1% BSA 5% gelatin 0.1% casein 0.05% polyethylene glycol 1.5% Tween-20 0.05% protease inhibitor complex 0.5% Proclin300 0.05%

[0101] The preparation process is as follows:

[0102] Preparation operation step 1: prepare biological buffer (phosphate buffer, pH7.4, 1000ml), weigh 1.44gNa 2 HPO 4、 0.24g KH 2 PO 4 , 8g NaCl, 0.2g KCl, dissolved in 1000ml of distilled water, after fully dissolved, adjust the pH value to 7.4.

[0103] Preparation operation step 2: Measure 100ml of biological buffer, weigh 0.1g of gelatin and 0.05g of case egg, and dissolve in a water bath at 60-80...

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Abstract

The invention discloses a preparation method and application of a ready-to-use antibody reagent (immunohistochemical), and relates to the field of pathological detection reagents. The universal antibody diluent comprises a biological buffer solution, a protein protective agent, a protein stabilizer, a polymer accelerator (sensitizer), a protein dispersant, an efficient protease inhibitor, a preservative and the like, so that the universal antibody diluent has the characteristics of high sensitivity, low background, clear dyeing and long period of validity.

Description

technical field [0001] The invention relates to the field of pathological detection reagents, in particular to a preparation method and application of a ready-to-use antibody reagent (immunohistochemistry). Background technique [0002] Immunohistochemistry (IHC) is an indispensable and important technical means in the current pathological diagnosis process, and has played an immeasurable role in tumor diagnosis, typing and identification, guiding medication, prognostic evaluation and efficacy evaluation. [0003] Six kinds of antibodies including TOP2A, P27KIP1, GST-pi, EpCAM, Podoplanin, and VEGFA are contained in extremely small amounts in ready-to-use antibody reagents (immunohistochemistry), and are very unstable when using traditional antibody diluents to prepare antibody reagents. Ready-to-use antibody reagents (immunohistochemistry) have a very short shelf life. [0004] Ready-to-use immunohistochemical antibody (protein) reagents generally exist in the form of solu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/53
CPCG01N33/6854G01N33/5306
Inventor 吕律杨林黄云坚
Owner 杭州迪英加科技有限公司
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