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Fusarium oxysporum FP26 and application thereof

A technology for layering Fusarium and FP26, applied in the field of microorganisms, to improve the quality and safety of agricultural products, eliminate production and accumulation, and control pollution

Active Publication Date: 2022-07-29
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on non-toxin-producing Fusarium biocontrol bacteria is in its infancy in China, and there is no case of successful promotion and application in the field.

Method used

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  • Fusarium oxysporum FP26 and application thereof
  • Fusarium oxysporum FP26 and application thereof
  • Fusarium oxysporum FP26 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Obtainment of Fusarium Strains FP26

[0047] 1. Collection and isolation of strain FP26

[0048] A sample of rice ear rot susceptibility was collected from a paddy field in Panjin, Liaoning, my country. The rice grains with ear rot disease were surface-sterilized with 70% ethanol, rinsed twice with sterile water, and dried with sterilized filter paper. The diseased-healthy junction of the glume was incised, placed on WA medium, and cultured at 28°C. After the colony was formed, the tip mycelium was picked and inoculated on a PDA plate for cultivation. After single spore isolation and purification, the strain FP26 was obtained. 2. Determination of Fumonisin FB1 Content of Strain FP26

[0049] (1) Preparation of samples to be tested

[0050] The strain FP26 was inoculated on PDA medium and cultured at 28°C for 7-8 days. Elute the conidia with sterile water and dilute to obtain a conidia concentration of 10 6 cells / mL of bacterial suspension.

[0051] (2)...

Embodiment 2

[0073] Example 2: Competitive inhibitory effect of Fusarium sp. FP26 without toxigenicity on F. sp. Fp9

[0074] The stratified Fusarium strain Fp9 that produces fumonisins described in the embodiment is recorded in "Sun Lei, Wang Ling, Liu Lianmeng, Hou Yuxuan, Li Qiqin, Huang Shiwen. Rice ear rot bacteria have strong virulence and high-yielding fumonisides. Screening of vegetarian strains. China Rice Science, 2018, 32(6): 610~616.” article. The colony morphology of stratified Fusarium strain Fp9 on PDA is as follows figure 2 shown.

[0075] Fusarium stratum strains FP26 that do not produce fumonisins and Fusarium stratum strains Fp9 that produce fumonisins were respectively inoculated on PDA solid medium and cultured at 28°C for 6 days. The non-toxic Fusarium stratum strain FP26 grows faster on PDA medium and can cover a petri dish with a diameter of 9 cm, with a spore production of 5.7×10 7 pcs / mL. Under the same conditions, the colony diameter of the toxin-producing F...

Embodiment 3

[0082] Example 3: Competitive inhibitory effect of Fusarium stratum strain FP26 on toxigenic Fusarium stratum in different regions

[0083] The Fusarium stratum fusarium that produces fumonisins isolated from different regions, its strain numbers are Fp39 (Hefei, Anhui), Fp153 (Zhengzhou, Henan), Fp387 (Shenyang, Liaoning), Fp417 (Taian, Shandong), Fp665 (Baoshan, Yunnan), respectively. Inoculated on PDA solid medium. After culturing at 28°C for 5 days, the conidia were washed with sterile water and diluted to obtain a conidia concentration of 10. 5 cells / mL of bacterial suspension.

[0084] The Fusarium strain FP26 from the non-toxic layer was inoculated on PDA solid medium. After culturing at 28°C for 7-8 days, the conidia were washed with sterile water and diluted to obtain a conidia concentration of 10 5 cells / mL of bacterial suspension.

[0085] In the treatment group, the bacterial suspensions of the non-toxin-producing Fusarium stratum FP26 and the toxin-producing F...

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Abstract

The invention discloses fusarium laminatum FP26 and application of the fusarium laminatum FP26. The fusarium oxysporum FP26 is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the fusarium oxysporum FP26 is CGMCC (China General Microbiological Culture Collection Center) No. 23809. The fusarium laminatum FP26 does not generate fumonisins and has the capacity of rapid growth and reproduction, and the fusarium laminatum FP26 can fight for limited ecological niche in a fumonisins susceptible area with a toxin-producing strain, so that growth, reproduction and toxin production of fumonisins producers can be efficiently inhibited, and formation of the fumonisins in crops can be remarkably reduced. The operation method is simple, the production and use cost is low, the method is environmentally friendly, formation of fumonisins is fundamentally removed in the early pollution stage, and the method has high application value and market potential for reducing fumonisins pollution in agricultural products and improving the quality of the agricultural products.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Fusarium stratumbilis FP26 and applications thereof. Background technique [0002] Fumonisins are a very common mycotoxins that endanger food safety in my country. At present, four clusters of fumonisins, FA, FB, FC, and FP, have been isolated. Among them, fumonisin FB1 accounted for 70% and was the most toxic. Fumonisin FB1 can cause equine leukomalacia, porcine pulmonary edema syndrome, hepatocyte apoptosis and nephrotoxicity in mice, and is considered to be related to the high incidence of esophageal cancer in humans, and has been designated as 2B by the International Agency for Research on Cancer. carcinogens. [0003] Fumonisins mainly contaminate rice, corn, wheat, sorghum and other crops, and can harm human and animal health through direct or indirect (animal product or feed processing) transmission. In recent years, many developed countries have for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C09K17/14C12R1/77
CPCC12N1/14C09K17/14C09K2101/00Y02A40/10
Inventor 王玲胡培松唐绍清魏祥进圣忠华焦桂爱谢黎虹邵高能胡时开
Owner CHINA NAT RICE RES INST
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