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Composite 3D printing ink and application thereof

A 3D printing and ink technology, applied in medical science, prosthesis, additive processing, etc., can solve the problems of uneven powder and poor degradability, uneven printing structure, increase process complexity, etc. Excellent biocompatibility, speed-increasing effect

Active Publication Date: 2022-07-29
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It mainly includes: ①Chemical modification of dECM materials, such as methacrylation modification, introducing double bonds, changing the cross-linking method to enhance its mechanical properties; It is a better method. For several systems commonly used at present, such as the bioink directly mixed with methacrylated gelatin and decellularized matrix (GelMA+dECM) hydrogel, it takes into account printability and biological ink to a certain extent. However, since GelMA undergoes a temperature-responsive sol-gel transition at a temperature below body temperature, while the acellular matrix hydrogel undergoes gelation at body temperature, the difference in the gelation mechanism and temperature-sensitive transition between the two is Extrusion printing increases the complexity of the process, and phase separation is prone to occur during the stretching and mixing process during the printing process, resulting in uneven printing structures and nozzle clogging; adding acellular matrix as a powder to GelMA has a positive effect on printability. Great lift, but uneven powder and poor degradability are not conducive to rapid action

Method used

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  • Composite 3D printing ink and application thereof
  • Composite 3D printing ink and application thereof
  • Composite 3D printing ink and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0046] 2. In the following examples, the acellular matrix powder used was prepared by the following method:

[0047] (1) Pretreatment: Cut the peripheral nerve tissue of mice into tissue blocks of 5mm×5mm×5mm, and wash them thoroughly with PBS solution;

[0048] (2) Decellularization: The peripheral nerve tissue was placed in a 3% Triton X 100 aqueous solution for 12 hours, rinsed three times in sterile distilled water, and then placed in a 4% sodium deoxycholate aqueous solution for 24 hours. Rinse 3 times in sterile distilled water. Repeat the above process twice;

[0049] (3) One-time freeze-drying: place the decellularized peripheral nerve tissue in a -80°C freeze-drying machine for freeze-drying;

[0050] (5) Degreasing: Use a mixed solvent of ethanol and dichloromethane to degrease the peripheral nerve tissue after one freeze-drying, wherein the volume ratio of ethanol and dichloromethane is 1:2. The bacteria are washed with distilled water for many times until the mi...

Embodiment 1

[0059] This example is used to illustrate the gelation properties of the peripheral nerve tissue acellular matrix of the present invention.

[0060] Weigh 10 mg of acellular matrix powder and digest it with 1 ml of pepsin solution to obtain a mass fraction of 1% acellular matrix solution. Adjust the pH of the acellular matrix solution to neutrality with NaOH solution to terminate the digestion, and add 10 × PBS solution to make The ionic strength of the acellular matrix solution was 1, and gelation at 37°C gave a mass fraction of 1% acellular matrix gel.

[0061] image 3 The physical image of the acellular matrix gel in this example, from image 3 It can be seen that the acellular matrix of peripheral nerve tissue can undergo sol-gel transition at 37°C and pH=7, from liquid state to solid state.

Embodiment 2

[0063] This example is used to illustrate the acellular matrix microspheres and the preparation method thereof, including the following steps:

[0064] (1) Preparation of microfluidic chip: After mixing the polydimethylsiloxane and the cross-linking agent in a mass ratio of 10:1, pour it on the master, where the cross-linking agent is sylgard 184 from Dow Corning, and then pour it on the master. Cured at 70℃ for 4h;

[0065] After curing, the polydimethylsiloxane mold is peeled off from the master, and then the channel inlet and channel outlet are punched out on the mold with a punch;

[0066] After punching, the polydimethylsiloxane mold is subjected to oxygen plasma treatment, adhered to the polydimethylsiloxane film, and finally cured at 70°C for 4 hours to obtain a microchannel chip;

[0067] (2) Preparation of the aqueous phase: The acellular matrix powder was added to the pepsin solution for digestion to obtain an acellular matrix solution with a mass fraction of 1%. Th...

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Abstract

The invention discloses composite 3D printing ink and application thereof, and belongs to the technical field of bio-ink. The composite 3D printing ink is mainly obtained by mixing acellular matrix gel microspheres and a gel material. The hydrogel microspheres are prepared from the acellular matrix and then added into the gel material to form the composite 3D printing ink, so that the printability and biological activity of the ink are effectively compatible, the acting speed of bioactive substances in the ink is increased, and the negative influence of phase separation of two hydrogels on the printing process is avoided; in addition, the method has the application potential that cells are packaged or loaded on microspheres for modular construction, and a multi-scale three-dimensional composite structure support is manufactured to adjust the microenvironment and the macroenvironment of the cells.

Description

technical field [0001] The invention belongs to the technical field of biological inks, and in particular relates to a composite 3D printing ink and applications thereof. Background technique [0002] Biological 3D printing technology is a new technological method that uses a computer three-dimensional model as a "drawing", assembles a special "bio-ink", and finally produces a three-dimensional structure. It has good application prospects and great social value in the field of tissue repair and regenerative medicine. . As the connection point between equipment and cells, bio-ink not only needs to quickly solidify while ensuring printing accuracy, but also protect cells during the printing process and provide a microenvironment that supports cell growth. It is the most important part of the printing process. . There are three requirements for bioinks, involving printability, biocompatibility, and mechanical properties. Printability refers to the performance of materials th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/18A61L27/22A61L27/50A61L27/54B33Y70/10B33Y80/00
CPCA61L27/3633A61L27/3604A61L27/3687A61L27/3691A61L27/18A61L27/222A61L27/50A61L27/54B33Y70/10B33Y80/00A61L2430/40C08L71/02
Inventor 全大萍储晗昱白莹张可鑫饶子龙
Owner SUN YAT SEN UNIV
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