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Paenibacillus polymyxa, biochemical preparation and application of paenibacillus polymyxa and biochemical preparation

A technology of Paenibacillus polymyxa and bacilli, which is applied in the field of disease prevention and control, can solve problems such as product safety and environmental pollution, and achieve the effects of good chemical drug compatibility, growth-promoting effect, and high effective number of viable bacteria

Pending Publication Date: 2022-07-12
MOON (GUANGZHOU) BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention provides Paenibacillus polymyxa microbial strains and products thereof, which integrate biological nematicide and phytobactericidal effects, have strong compatibility and are easy to industrialize, expand the product types and efficacy of Paenibacillus polymyxa, and enrich the The quantity and preparation type of nematode-killing microbial pesticide products also solve the problems of product safety and environmental pollution in the chemical control methods used to control root-knot nematode and various soil-borne diseases in the prior art

Method used

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  • Paenibacillus polymyxa, biochemical preparation and application of paenibacillus polymyxa and biochemical preparation
  • Paenibacillus polymyxa, biochemical preparation and application of paenibacillus polymyxa and biochemical preparation

Examples

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Embodiment 1

[0051] This example provides methods for the isolation, purification and identification of Paenibacillus polymyxa M886 strain.

[0052] 1. Isolation and purification of Paenibacillus polymyxa M886 strain

[0053] The samples were collected from the rhizosphere soil of rice in Guangdong Province.

[0054] Weigh 4 g of soil sample, add it to 36 mL of sterilized Tween water with a concentration of 0.1% (v / v), and vortex for 10 min to obtain 10 -1 concentration diluent. Dilute the soil liquid gradient to 10 with Tween water -4 , 10 -5 and 10 -6Concentration, then coated on LB solid medium, cultivated in a constant temperature incubator at 30 °C, picked a single colony after 1 day, continued to inoculate on LB solid medium, and cultured upside down in a constant temperature incubator at 30 °C for 1 day, and then The morphology of the colonies was observed, and the morphology of the cells was observed with an optical microscope (1000×).

[0055] LB solid medium: tryptone 10g, ...

Embodiment 2

[0060] This example provides a method for preparing a fermented culture of Paenibacillus polymyxa M886 strain and its effective viable count.

[0061] After the M886 strain was activated, liquid fermentation was carried out with a liquid medium to obtain a fermentation culture. The fermentation culture method is simple and easy to operate, and the M886 strain is easy to be activated and fermented and cultured, and is extremely suitable for industrial production. The fermentation method is as follows:

[0062] The M886 strain stored in the -80°C refrigerator was coated on the LB plate by the coating method (recipe: tryptone 10g, yeast powder 5g, NaCl 10g, agar powder 15g, replenish water to 1L, 121°C high pressure steam sterilization for 20 minutes) , cultured at 30°C for 1 day. Pick a single colony of M886 grown on the LB plate and inoculate it in LB liquid medium (recipe: peptone 10g, yeast powder 5g, sodium chloride 5g, glucose 1g, add water to 1L, sterilize at 121°C for 2...

Embodiment 3

[0067] In this example, an experiment was carried out on the lethal effect of Paenibacillus polymyxa M886 strain on the second instar larvae of root knot nematodes in vitro.

[0068] 1. Preparation of Nematodes for Testing

[0069] Root knot nematodes were collected from the greenhouse of the Agriculture Group of Moon (Guangzhou) Biotechnology Co., Ltd. Take out the root system of the cucumber with root knot nematode disease, rinse it gently with water, carefully remove the egg mass from the surface of the root system, put it in 0.5% sodium hypochlorite for disinfection for 3 minutes, rinse it with sterile water 3 times, and put it in a small amount of sterile water. In a petri dish, cultured in a 25°C incubator, and collected the hatched second-instar larvae of Root knot nematodes for 24h-48h, and suspended them in sterile water for experimental research.

[0070] 2. Nematicidal effect of fermentation supernatant of strain M886

[0071] The fermentation culture of strain M8...

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Abstract

The invention discloses paenibacillus polymyxa, a biochemical preparation and application of the paenibacillus polymyxa, and relates to the technical field of disease control. The bacterial strain of the paenibacillus polymyxa species is preserved in Guangdong Microbial Culture Collection Center on January 15, 2021, and the preservation number is GDMCC No: 61436. The invention develops the paenibacillus polymyxa microbial strain which integrates the effects of biologically killing nematodes and phytopathogens and is easy to industrialize and a product thereof, so that the problems of product safety, environmental pollution and the like existing in a chemical prevention and treatment method adopted for preventing and treating root knot nematode diseases and various soil-borne diseases in the prior art are solved. Meanwhile, the paenibacillus polymyxa also has the effect of regulating plant growth.

Description

technical field [0001] The invention relates to the technical field of disease prevention and control, in particular to a Paenibacillus polymyxa, biochemical preparations and applications thereof. Background technique [0002] Meloidogyne spp. is a type of plant pathogenic nematode that seriously threatens the world's agricultural production. Nearly 100 species of root-knot nematodes have been reported, and more than 3,000 species of root-knot nematodes can be parasitized. Common root knot nematodes are Meloidogyne incognita, Meloidogyne hapla, Meloidogyne javanica and Meloidogyne arenaria. Among them, there are many reports of suffering from root-knot nematode, which can lead to a 10% reduction in plant yield, and even more than 75% in severe cases. The currently registered nematicidal pesticide formulations are still mainly chemical, and their adverse effects on the environment, yield and product quality are becoming more and more serious, which has induced the market's d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/25A01N43/90A01N47/34A01N47/18A01N59/20A01N57/32A01P1/00A01P3/00A01P5/00A01P21/00C05G3/00C05G3/60A01C1/06A01C1/08C12R1/01
CPCA01N63/25A01N43/90A01N47/34A01N47/18A01N59/20A01N57/32C12N1/20C05G3/00C05G3/60A01C1/06A01C1/08Y02A40/10
Inventor 王雪王琳王科晶梁锏文蒋先芝余昕彤曹鸿一季晓琴王杰刘仁燕
Owner MOON (GUANGZHOU) BIOTECH CO LTD
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