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Tree shrew testicular interstitial cell isolated culture method and application thereof

A technology for the separation and culture of Leydig cells, which is applied in the field of separation and culture of Leydig cells in tree shrew, can solve the problems of few reports on the separation and culture of LCs.

Pending Publication Date: 2022-07-08
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the isolation and culture of LCs is mainly concentrated on mammals such as rats, cattle and sheep, but there are few reports on the isolation and culture of LCs of tree shrews, a new experimental animal.

Method used

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  • Tree shrew testicular interstitial cell isolated culture method and application thereof
  • Tree shrew testicular interstitial cell isolated culture method and application thereof
  • Tree shrew testicular interstitial cell isolated culture method and application thereof

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Embodiment Construction

[0036] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention. If no specific technology or condition is indicated in the examples, the technology or condition described in the literature in the field or the product specification is used. The reagents or instruments used without the manufacturer's indication are conventional products that can be obtained through purchase.

[0037] Unless otherwise stated in the present invention, the percent sign represents the mass fraction. The ratio is the mass ratio, and the concentration is the mass concentration.

[0038] Materials and Methods

[0039] 1.1 Experimental animals

[0040] Two 3-month-old male Chinese Burmese tree shrew, w...

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Abstract

The invention relates to an isolated culture method and application of tree shrew testicular interstitial cells. The method comprises the steps of separation and purification. According to the invention, the isolated culture method of the tree shrew testicular interstitial cells is successfully established, the susceptibility and virus proliferation characteristics of Zika virus to the tree shrew testicular interstitial cells are determined, and a basis is provided for research on a Zikv invasion reproductive system mechanism.

Description

technical field [0001] The invention relates to the field of cell culture and application, in particular to a method for separating and culturing Leydig cells of tree shrew and application thereof. Background technique [0002] Zika virus (Zika virus, Zikv) is a single-stranded positive-stranded RNA virus, which is mainly transmitted through the bite of virus-infected Aedes mosquitoes. Zika virus can target and infect neural precursor cells, inhibit the proliferation of neural stem cells and cause their differentiation. Abnormal, and eventually lead to the thinning of the cerebral cortex, the enlargement of the volume of the brain cavity, and the appearance of microcephaly. In addition, studies have shown that infection with Zika virus can cause inflammation in the testes of mice. In severe cases, it will cause damage to the internal structure of the testis and massive cell death, posing a potential threat to male reproductive health, but the related infection mechanism is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071G01N33/543C12Q1/02A61P15/08A61K35/52
CPCC12N5/0683G01N33/543G01N33/5005A61K35/52A61P15/08C12N2509/00C12N2509/10Y02A50/30
Inventor 代解杰邓伟罕园园王文广陆彩霞李娜孙晓梅仝品芬
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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