Molecular information detection method and system based on paramagnetic resonance of nano-diamond probe

Abstract

The invention discloses a molecular information detection method and system based on paramagnetic resonance of a nano-diamond probe, and the method comprises the steps: a to-be-detected sample is fixed on a precision displacement device, the to-be-detected sample comprises a glass slide containing to-be-detected molecules and a nano-probe, and the nano-probe comprises the nano-diamond probe with a nitrogen vacancy color center; exciting the electronic state of the nanoprobe by using a confocal microscopy device; a preset amplitude modulation signal emitted by the microwave system is radiated to the nanoprobe by using a radiation assembly on the precise displacement device, so that the nanoprobe and the to-be-detected molecule resonate to generate a fluorescence signal; collecting a fluorescence signal through a confocal microscopic device; and processing the fluorescence signal by using a fluorescence signal processing system to obtain the molecular information of the to-be-detected molecule. Amplitude modulation microwaves are applied to the nanoprobe, so that the resonance condition of the nanoprobe and target spinning does not depend on angles any more, and the problems of spectral line broadening and distortion caused by probe movement are solved.

Description

technical field [0001] The invention relates to the technical field of magnetic resonance measurement, in particular to a molecular information detection method and system based on nanodiamond probe paramagnetic resonance. Background technique [0002] Optically Detected Magnetic Resonance (ODMR) is an optically-based electron spin magnetic resonance technique by which electron spins in crystal defects can be optically pumped for initialization and readout. Nitrogen-Vacancy color center, referred to as NV color center, is a defect structure in diamond with good and stable optical properties. [0003] The electron spin in the middle of the diamond NV color center can be manipulated by optical detection magnetic resonance technology, and the spin state of the electron can be obtained by detecting the fluorescence intensity of the diamond NV color center. Thereby, the electron paramagnetic resonance spectrum and the related properties of the external environment can be obtaine...

AI Technical Summary

Problems solved by technology

However, nanodiamonds may rotate under physiological conditions in the cell, and the quantization axis of the NV color center will also change, which makes it difficult to manipulate it with the existing electron paramagnetic resonance method.

Secondly, the coherence time of nanoprobes in nanodiamonds is short, which limits the magnetic sensitivity of nanoprobes in nanodiamonds, making it difficult to be practically applied in electron paramagnetic resonance measurements.

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