Bifidobacterium longum CCFM1206 capable of producing sulforaphane and relieving inflammation

A CCFM1206, Bifidobacterium longum technology, applied in the field of microorganisms, to achieve the effect of improving colonic barrier function, increasing the content and reducing the level

Pending Publication Date: 2022-06-24
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no strain that can effectively convert glucoraphanin into sulforaphane and fully exert anti-inflammatory activity

Method used

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  • Bifidobacterium longum CCFM1206 capable of producing sulforaphane and relieving inflammation
  • Bifidobacterium longum CCFM1206 capable of producing sulforaphane and relieving inflammation
  • Bifidobacterium longum CCFM1206 capable of producing sulforaphane and relieving inflammation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1: Screening, identification, observation and preservation of Bifidobacterium longum CCFM1206.

[0073] 1. Filter

[0074] Take 0.5 g of fresh fecal samples from healthy adults and add them to 4.5 mL of 0.9% normal saline for gradient dilution. Select the appropriate gradient dilution and spread it in MRS solid modified medium supplemented with 0.2% bromocresol purple. Incubate under anaerobic conditions at 37°C for 24-48h. A single colony whose discoloration circle obviously turned yellow was inoculated onto an MRS plate for streaking and purification, and a single colony was picked and transferred to a liquid MRS liquid medium for enrichment, and stored in 30% glycerol to obtain the strain Bifidobacterium longum CCFM1206.

[0075] 2. Identification

[0076] The whole genome DNA of strain CCFM1206 was extracted for the amplification of 16S rDNA, and the amplified DNA fragments were collected and sequenced (completed by Suzhou Jinweizhi Biotechnology Co., Ltd....

Embodiment 2

[0081] Example 2: Fermentation of Bifidobacterium longum CCFM1206 to produce sulforaphane

[0082] Bifidobacterium longum CCFM1206 stored at -80°C was streaked in MRS solid medium, cultured anaerobic at 37°C for 24 to 48 hours, and then passaged in MRS liquid medium for 2 to 3 times. The inoculum was inoculated into the modified MRS liquid medium, and cultured anaerobic at 37 °C for 24 h, and the obtained fermentation broth was used for the detection of sulforaphane content.

[0083] The result is as figure 2shown. The inoculated medium did not contain sulforaphane, and the content of sulforaphane was 16.76 μM detected after 24 hours of fermentation by Bifidobacterium longum CCFM1206.

Embodiment 3

[0084] Example 3: Effects of Bifidobacterium longum CCFM1206 and glucoraphanin-containing diet on disease symptoms in UC mice:

[0085] Forty 6-week-old healthy male C57BL / 6J mice were randomly divided into 5 groups with 8 mice in each group after one week of acclimatization. The 5 groups were blank group, model group, glucoraphanin-containing diet group (BSE), Bifidobacterium longum CCFM1206 group (CCFM1206), and glucoraphanin-containing diet compound Bifidobacterium longum CCFM1206 group (BSE+CCFM1206).

[0086] Days 8-21 were the 14-day gavage intervention period. The dose of each gavage was 0.2 mL per animal, and the gavage time was the same every day. Among them, the control group and model group were intragastrically administered with normal saline, the BSE group was intragastrically administered with 40 mg / mL broccoli seed aqueous extract solution, and the CCFM1206 group was intragastrically administered with 5 × 10 9 CFU / mL bacterial suspension, BSE+CCFM1206 group con...

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Abstract

The invention discloses bifidobacterium longum CCFM1206 capable of producing sulforaphane and relieving inflammation, and belongs to the technical field of microorganisms. The bifidobacterium longum CCFM1206 provided by the invention can be used for converting the glucoraphanin into the sulforaphane and promoting the metabolism of the glucoraphanin and the generation of the sulforaphane in a mammal body. The bifidobacterium longum CCFM1206 disclosed by the invention can be independently used or combined with glucoraphanin for relieving weight loss during ulcerative colitis, reducing release of colon proinflammatory factors, improving a colon barrier function, relieving splenomegaly caused by systemic inflammation and reducing the level of proinflammatory factors in serum; the content of proinflammatory factors in liver tissues is reduced, the content of anti-inflammatory factors is increased, and the content of short-chain fatty acid is increased.

Description

technical field [0001] The invention relates to a Bifidobacterium longum CCFM1206 that produces sulforaphane and can relieve inflammation, and belongs to the technical field of microorganisms. Background technique [0002] Sulforaphane, molecular formula C 6 H 11 NOS 2 , is an isothiocyanate, a secondary metabolite of glucosinolates (mainly glucoraphanin) in cruciferous plants. However, sulforaphane usually does not exist in natural plants, but exists stably in plants in the form of its precursor substance, glucoraphanin. Only when plant tissue is damaged, glucoraphanin is hydrolyzed with myrosinase to produce sulforaphane. The hydrolysis process is affected by various factors such as pH, temperature, moisture, etc., resulting in a decrease in yield. In addition, sulforaphane itself is not stable and very volatile, so it is difficult to extract sulforaphane from natural plants. [0003] Broccoli, cabbage, cabbage and other cruciferous vegetables are reported to be rich...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P13/00A61K35/745A61K36/31A61K9/19A61P1/04A61P1/00A61P29/00A23C9/123A23C9/13A23L2/38A23L19/00A23L19/20A23L29/00A23L33/105A23L33/135C12R1/01A61K31/7028
CPCC12N1/20C12P13/002A61K35/745A61K31/7028A61K36/31A61K9/19A61P1/04A61P1/00A61P29/00A23L33/135A23L33/105A23L29/065A23C9/1234A23C9/1307A23L19/09A23L19/20A23L2/382A23V2002/00A23V2400/533A61K2300/00A23V2200/32A23V2200/3204Y02A50/30
Inventor 毛丙永邬佳颖崔树茂唐鑫张秋香赵建新陈卫
Owner JIANGNAN UNIV
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