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Denitrification microbial agent with broad-spectrum dissolved oxygen and organic carbon tolerance and application thereof

A technology of dissolved oxygen and organic carbon, used in aerobic and anaerobic process treatment, bacteria, chemical instruments and methods, etc.

Active Publication Date: 2022-06-07
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problem of water pollution treatment that organic carbon and dissolved oxygen in polluted water inhibit biological denitrification activity, and provides a denitrification functional bacterial agent with tolerance to dissolved oxygen and organic carbon, so as to realize efficient removal of ammonia nitrogen and total nitrogen in water

Method used

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  • Denitrification microbial agent with broad-spectrum dissolved oxygen and organic carbon tolerance and application thereof
  • Denitrification microbial agent with broad-spectrum dissolved oxygen and organic carbon tolerance and application thereof
  • Denitrification microbial agent with broad-spectrum dissolved oxygen and organic carbon tolerance and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Alcaligenes faecalis CHO6 and Bacillus amyloliquefaciens The specific separation and breeding process of yel14 is as follows:

[0041] (1) Alcaligenes faecalis CHO6 isolation and culture:

[0042] provided by the present invention Alcaligenes faecalis CHO6 is derived from the water body sediment of Shijing River, Guangzhou City, Guangdong Province, which is polluted by industry. Published in the literature: Qian Y. F., Xu M. Y., et al. Synergistic interactions of Desulfovibrio and Petrimonas for sulfate-reduction couplingpolycyclic aromatic hydrocarbon degradation. J. Hazard. Mater., 2021, 407) Single-cell screening of rod-shaped microorganisms ( Figure 7 ), placed in the liquid medium for 20 days, and observed whether the bacterial solution was turbid (30°C, 10% H 2 +10%CO 2 +80%N 2 anaerobic box), the turbid bacterial liquid was streaked on the agar solid plate of the above medium and identified. Alcaligenes faecalis The colonies of CHO6 wer...

Embodiment 2

[0048] Alcaligenes faecalis CHO6 and Bacillus amyloliquefaciens Validation of nitrogen removal ability of yel14 in common medium:

[0049] According to the separation of the two strains in different media, two different basal media were selected to verify the denitrification ability, namely R 2 A and PDB medium. Taking the inoculum volume fraction as 1%, the Alcaligenes faecalis CHO6 was inoculated in 1 liter of medium R 2 A(12 g / L)+NO 2 - (17.5 mM)+NH 4 + (3.5 mM) to verify its effect on NH 4 + Oxidative capacity and response to NO 2 - the reducing ability while inoculated in 1 L of medium R 2 A(12 g / L)+ NO 3 - (16 mM), the denitrification ability of strain CHO6 was verified. And need to be under aerobic (180 rpm, 30 ℃) conditions and anaerobic (30 ℃, filled with 99.99% N) 2 The denitrification ability of CHO6 was verified under the condition of anaerobic box. Among them, NO 3 - , NO 2 - and NH 4 + The ion concentration was determined by ion chrom...

Embodiment 3

[0056] Alcaligenes faecalis strain CHO6 and Bacillus amyloliquefaciens The application of strainyel14 in landfill leachate is as follows:

[0057] The original solution was retrieved from the Huizhou Landfill Leachate Treatment Plant (Yonglian Road, Huicheng District, Huizhou City, Guangdong Province, mature type, > 10 years) and stored in a -4°C cold storage until use. In order to verify Alcaligenes faecalis CHO6 and Bacillus amyloliquefaciens The effect after compounding of yel14, the strains CHO6 and yel14 were cultured to the logarithmic growth phase, and the bacteria group CHO6-yel14 compounded at a volume ratio of 1:1 was inoculated into 1 liter of landfill leachate stock solution, and the inoculation amount was the volume fraction. 1%. The landfill leachate without strain CHO6 or yel14 was set as blank control group (CK), and the culture method was set as follows:

[0058] Aerobic culture: CK (3 groups), CHO6 (3 groups), yel14 (3 groups), CHO6-yel14 (3 group...

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Abstract

The invention discloses a denitrification bacterial agent with broad-spectrum dissolved oxygen and organic carbon tolerance and application thereof. The microbial inoculum comprises bacillus amyloliquefaciens del14 and alcaligenes faecalis CHO6. The strain tel14 has both a heterotrophic ammonia oxidation function and an aerobic denitrification function, and the strain CHO6 has a denitrification function under both anaerobic and aerobic conditions. Compared with the verification result of independently inoculating the strains del14 and CHO6 to the landfill leachate, the compound flora CHO6-del14 has a stronger denitrification function. The microbial agent is put into wastewater containing nitrate nitrogen, nitrite nitrogen and ammonia nitrogen and is uniformly mixed, so that the effect of efficiently removing total nitrogen is achieved. According to the present invention, the bacterial agent developed by using the bacterial strains del14 and CHO6 can achieve the whole-process nitrogen removal of NH4 < + >-NO3 <->-NO2 <->-N2 in the polluted water body under the aerobic and anaerobic conditions, and has characteristics of simple operation, low cost and environmental protection.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and specifically relates to a strain of Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) yel14 and a strain of Alcaligenes faecalis ( Alcaligenes faecalis ) Breeding of CHO6 and the bacterial preparation composed of these two microorganisms and its application in water denitrification. Background technique [0002] In the process of rapid development of industrialization and urbanization, the problem of nitrogen pollution in water environment is becoming more and more serious. Since the nitrogen cycle is closely coupled with the cycle of various substances, it is also an important factor in determining the degree of eutrophication of water bodies. Therefore, the denitrification efficiency of polluted water bodies has received much attention. Biological nitrogen removal technology has become a commonly used method in the field of water pollution control due to it...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F3/30C12N1/20C02F101/16C02F103/06C12R1/07C12R1/05
CPCC02F3/34C02F3/302C12N1/20C02F2101/16C02F2103/06C02F2101/163Y02W10/10
Inventor 许玫英周少锋杨旭楠杨永刚陈杏娟
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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