Juglans mandshurica flavone component nanoemulsion preparation as well as preparation method and application thereof

A technology of the walnut flavonoid group and the walnut flavone, which is applied in the field of nano-emulsion preparation of the walnut flavonoid component and its preparation, can solve the problems of low water solubility and poor bioavailability of the walnut flavonoids, and achieve strong anti-tumor effect on antioxidant capacity

Pending Publication Date: 2022-05-10
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the present invention, the preparation process of the nanoemulsion of the flavonoid components of the walnut tree is relatively simple, and the average particle diameter of the obtained nano-preparation is 20-35nm, which solves the problems of low water solubility and poor bioavailability of the flavonoids of the walnut tree

Method used

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  • Juglans mandshurica flavone component nanoemulsion preparation as well as preparation method and application thereof
  • Juglans mandshurica flavone component nanoemulsion preparation as well as preparation method and application thereof
  • Juglans mandshurica flavone component nanoemulsion preparation as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Optimization of preparation process of walnut catalpa flavone component nanoemulsion

[0023] (1) Oil phase screening

[0024] Weigh 6 g of Tween 80 and Span 80 compound surfactant (4.6:1), 2 g of absolute ethanol, that is, 8 g of mixed surfactant in total. Add 1 g of various oil phases (castor oil, olive oil, isopropyl myristate, isopropyl palmitate and sweet almond oil) under a constant temperature magnetic stirrer at 25°C, stir for 1 hour, then slowly drop in distilled water, if the system It may become clear or may be considered as a nanoemulsion. If multiple systems form nanoemulsions, then the best oil phase is further screened by constructing a pseudo-ternary phase diagram. The results show that both isopropyl myristate (IPM) and isopropyl palmitate (IPP) can form clear and transparent nanoemulsions, and finally select isopropyl myristate (IPM) as the nanoemulsion based on the pseudo-ternary phase diagram. The oil phase of the milk preparation. ...

Embodiment 2

[0033] Embodiment 2: Characterization of the nanoemulsion of the flavonoids component of Juglans catalpa

[0034] (1) Preparation of nano-emulsion of flavonoid components of walnut catalpa

[0035] After making a blank nanoemulsion according to the optimized prescription, take 10 g of the blank nanoemulsion and slowly add 220 mg of the purified product of walnut flavonoids to it, stir to make it completely dissolve, and make a nanoemulsion of the walnut flavone component with a content of 22.0 mg / g .

[0036] (2) Particle size analysis of the nanoemulsion preparation of the flavonoid components of the walnut catalpa

[0037] The particle size of the emulsion droplet is an important characteristic parameter of the nanoemulsion, and it is one of the most important properties to evaluate the stability of the nanoemulsion. The particle size of the sample is measured by a laser particle size analyzer. The results showed that the average particle size of the nanoemulsion of flavon...

Embodiment 3

[0042] Example 3: Research on the Anti-liver Cancer Effect of Nanoemulsion of Flavone Components of Juglans

[0043] (1) Cell line source and culture method

[0044] Culture HepG2 and HL02 cells according to conventional methods, specifically, in DMEM medium containing penicillin / streptomyces (1%) and fetal bovine serum (10%), in an incubator at 37°C and 5% CO2 .

[0045] (2) Effects of nanoemulsion of flavonoids from Juglans catalpa on the morphology of HepG2 cells

[0046] HepG2 cells in the logarithmic phase of growth were selected, and 3 × 10 5 Cells were inoculated in 6-well plates, cultured in an incubator for 24 h, and then discarded. After being treated with different concentrations of nanoemulsions of flavonoids from walnut catalpa (40, 80, 120 μg / mL) for 48 hours, the cell morphology was observed under an inverted microscope. As the drug concentration increases, the arrangement of the cells becomes loose, the cells are elongated, and appear as irregular polygons ...

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Abstract

The invention provides a juglans mandshurica flavone component nanoemulsion preparation and a preparation method and application thereof.The preparation method comprises the steps that a surfactant and a cosurfactant are mixed to prepare a mixed surfactant, then an oil phase is added into the mixed surfactant and mixed to be uniform, the mixture is stirred for 1 h through a constant-temperature magnetic stirrer, and the nanoemulsion is obtained. The preparation method comprises the following steps of: adding the flavone component of the juglans mandshurica into a water bath, adding distilled water drop by drop in a magnetic stirring state until a nano-emulsion with clear and transparent appearance is formed, then adding the flavone component of the juglans mandshurica, and dissolving by magnetic stirring to obtain the nano-emulsion of the flavone component of the juglans mandshurica. The method can be applied to various industries such as medical health, food health care and cosmetics.

Description

technical field [0001] The invention belongs to the field of medicine, and discloses a nanoemulsion preparation of the flavonoid component of walnut catalpa and its preparation method and application. Background technique [0002] Liver cancer is one of the most common malignant tumors worldwide. Among malignant tumors in my country, the incidence and mortality of liver cancer rank fourth and second respectively. However, due to the complex pathogenesis of liver cancer and the insidious onset, most patients have entered the middle and late stages when they are diagnosed, and the treatment options are extremely limited. In recent years, traditional Chinese medicinal materials have gradually attracted the attention of scientific researchers because of their unique advantages such as multi-target, multi-level, and less adverse reactions. A large number of research reports suggest that natural, effective, and low-toxic chemical components of traditional Chinese medicine have g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/52A61K9/107A61K47/26A61K47/10A61K47/14A61P35/00A61P39/06A61K8/06A61K8/37A61K8/49A61K8/86A61K8/9789A61Q19/00A23L33/105
CPCA61K36/52A61K9/1075A61K47/26A61K47/10A61K47/14A61P35/00A61P39/06A61K8/068A61K8/9789A61K8/86A61K8/4993A61K8/37A61Q19/00A23L33/105A61K2800/413A23V2002/00A23V2200/30A23V2250/2116
Inventor 林瑞新杜林娜初国栋武家成杨晶万辰梦
Owner JILIN UNIV
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