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Application of amaranthus caudatus crude polysaccharide in preparation of anti-hepatoma drugs

A kind of technology of crude polysaccharides and amaranth, which is applied in the field of crude polysaccharides of amaranthus and its preparation of anticancer drugs, and can solve problems such as side effects, liver toxicity, and immune deficiency

Pending Publication Date: 2022-05-06
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although traditional treatment methods can prolong the life of patients, relapses still occur, especially radiotherapy and chemotherapy are accompanied by serious side effects, including immunodeficiency, cell damage, liver toxicity, etc.

Method used

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  • Application of amaranthus caudatus crude polysaccharide in preparation of anti-hepatoma drugs
  • Application of amaranthus caudatus crude polysaccharide in preparation of anti-hepatoma drugs
  • Application of amaranthus caudatus crude polysaccharide in preparation of anti-hepatoma drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 is an example of extracting and preparing the crude polysaccharide of Amaranthus aurantium by water extraction and alcohol precipitation method, and Examples 2-7 are examples of the application of the crude polysaccharide of Amaranthus amaranth.

[0045] The extraction and mensuration of embodiment 1ACLP

[0046] 1. The extraction method of ACLP is as follows:

[0047] S1. Freeze-dry the amaranth fringe, grind it into powder, and pass through a 60-mesh sieve.

[0048] S2. Take 100g of amaranth powder and add the extraction reagent water, the ratio of solid to liquid is 1:30, use ultrasonic-assisted extraction for 1 hour, and repeat the extraction of filter residue once; combine the extracts, then carry out vacuum filtration, and use a rotary evaporator for the supernatant Concentrate under reduced pressure to 1 / 6 of the original volume (about 800-1000 mL of 1 / 6 of 5.5 L of the two extractions).

[0049] S3. Collect the concentrated solution, add 3 times the ...

Embodiment 2

[0054] Example 2 Effect of ACLP on liver cancer HepG2 and normal L02 cytotoxicity

[0055] (1) Human liver cancer HepG2 cells were obtained from Procell, and the cell culture medium was MEM complete medium containing 10% fetal bovine serum, 1% penicillin-streptomycin and 1% L-glutamine. L02 cell culture medium is RPMI1640 complete medium added with the same medium. Place cells at 37 °C, 95% air and 5% CO 2 cultured in an incubator with daily replacement of the medium. When the cell count density is about 90%, conduct one passage at the ratio of 1 to 5.

[0056] (2) Take the cells in the logarithmic growth phase and adjust the cell density to 3×10 4 cells / mL, inoculated evenly into 96-well plates, and made 6 parallels for each concentration. Place in an incubator to adhere to the wall for 24 hours, add different concentrations of ACLP (0, 50, 100, 200, 400, 800 μg / mL), continue to cultivate for 24 hours and 48 hours, add 10 μ CCK-8 reagent to each well, and measure at 450 n...

Embodiment 3

[0058] Example 3 Effect of ACLP on ROS Release of Liver Cancer HepG2 Cells

[0059] Cells in the logarithmic growth phase were taken, and the cell density was adjusted to 3×10 5 cells / mL, inoculated evenly into 6-well plates, and made 4 parallels for each concentration. After 24 hours of adherent culture in an incubator, different concentrations of ACLP (0, 50, 100, 200, 400, 800 μg / mL) were added, and the culture was continued for 48 hours. Cells were lysed on ice with inhibitor-free lysate for 30 minutes, the lysate was collected in a centrifuge tube, and the amount of intracellular reactive oxygen species was detected using an ELISA kit.

[0060] The results showed that ACLP significantly increased the release level of ROS in a dose-dependent manner. Such as figure 2 As shown, after ACLP treatment for 48h, the intracellular ROS level increased significantly from 100μg / mL.

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Abstract

The invention discloses an application of crude amaranthus caudatus polysaccharide in preparation of anti-liver cancer drugs. According to the research of the invention, the amaranthus caudatus crude polysaccharide can obviously inhibit liver cancer cells and / or promote apoptosis of the liver cancer cells, has a cytotoxic effect on the liver cancer cells, causes the rise of ROS and MDA contents of the liver cancer cells, and reduces the levels of GSH and CAT; the amaranthus caudatus crude polysaccharide is used for down-regulating Nrf2 protein, up-regulating p-p38, p-ERK and p-JNK protein, retarding cell cycle Cyclin D1 and c-Myc protein, changing mitochondrial membrane permeability and inducing apoptosis of liver cancer cells through ROS (reactive oxygen species); no toxic effect is caused on human normal cells; therefore, the compound has a very good application value in the aspect of preparing anti-hepatoma drugs.

Description

technical field [0001] The invention relates to the technical field of polysaccharide medicine application, and more specifically, relates to a kind of crude polysaccharide of amaranth amaranthus and its application in preparing anticancer medicine. Background technique [0002] Amaranth (Amaranthus caudatus L.), also known as Xueyingzi, is commonly known as Xianrengu, Laoqianggu, Laocanggu, etc. It belongs to the genus Amaranthus (Amaranthaceae) of the Angiospermae (Angiospermae) and is an annual. herb. Amaranth amaranth originated in temperate, subtropical and tropical regions, and is now widely distributed throughout my country. Its leaves are rich in protein, lipids, vitamins, minerals, dietary fiber and other nutrients, can be eaten as vegetables, and have nourishing and strong effects, and are called "Viagra vegetables", "Chinese spinach" and so on; In addition to the various nutrients of traditional grains, the grain is also rich in lysine, which is scarce in tradit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61K36/21A61P35/00
CPCA61K36/21A61K31/715A61P35/00
Inventor 柳春红韩文娜刘焕李中意朱思俞
Owner SOUTH CHINA AGRI UNIV
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