Geobacterium engineering strain for passivating heavy metals and construction method of geobacterium engineering strain

Abstract

The invention discloses a geobacter engineering strain for passivating heavy metals and a construction method of the geobacter engineering strain, the geobacter engineering strain for passivating the heavy metals is Geobacter sulfurreducens HMP-1, and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO.62189. The invention further discloses a construction method of the geobacter engineering strain for passivating the heavy metals. According to the heavy metal passivated geobacterium engineering strain, cysteine is marked on the pilus surface, and the cysteine in the pilus is combined with heavy metal ions to quickly form metal sulfide precipitates, so that the heavy metal removal rate reaches 70% or above under the condition of not depending on any substrate or highly enriched heavy metal plants, and the heavy metal removal rate can reach 70% or above under the condition of not depending on any substrate or highly enriched heavy metal plants. Therefore, the problem of Cd < 2 + > residue in the environment can be effectively improved, and the defects that the existing microbial remediation effect on heavy metals is unstable and the remediation process is limited by many conditions are overcome.

Description

technical field [0001] The invention belongs to the field of bioremediation of heavy metals, and in particular relates to a Geobacter engineering strain for inactivating heavy metals and a construction method thereof. Background technique [0002] According to the survey, heavy metal pollutants are currently the main pollutants in the global soil. The soil environment directly determines the yield and quality of agricultural products. Some heavy metal pollutants can even directly endanger human health through bioaccumulation. Therefore, soil metal pollution cannot be carried out. The management of things is taken lightly. In the context of today's increasing sustainable agricultural development, seeking efficient, stable and environmentally friendly heavy metal pollution remediation technology is also the focus and difficulty of ensuring the safety of agricultural products. [0003] At present, microbial passivation technology has become a priority for heavy metal pollution...

AI Technical Summary

Problems solved by technology

For this reason, the present invention proposes a Geobacter engineering bacterial strain that inactivates heavy metals and its construction method. The Geobacter sulfurreducens HMP-1 constructed in the present invention can overcome the unstable and repairing effect of existing microorganisms to repair heavy metals. There are many disadvantages such as limited process conditions, and the purpose of reducing the toxicity of heavy metals is achieved by converting heavy metal ions into heavy metal sulfide precipitation, which has extremely high application value

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