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Mineralization reagent for shielding erythrocyte surface antigen and application thereof

A surface antigen and erythrocyte technology, applied in the modification of cell membrane, animal cells, vertebrate cells, etc., can solve the problems that the physiological functions of erythrocytes cannot be fully preserved, the shielding effect of erythrocyte surface antigens is limited, and the erythrocyte surface antigens cannot be completely shielded. Reduced binding, highly controllable size and shape, low toxicity

Pending Publication Date: 2022-04-19
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The properties and structural characteristics of the shielding layer material are the key factors affecting the effect of "universal blood". The compatibility is poor, the effect is not ideal, and the shielding effect of the red blood cell surface antigen is limited, which will cause certain immunity and hemolytic reactions, etc., resulting in the defects that the red blood cell surface antigen cannot be completely shielded and the red blood cell physiological function cannot be fully preserved in the prior art. Therefore, It is of great significance to develop a new technical route to prepare red blood cells that can shield surface antigens

Method used

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  • Mineralization reagent for shielding erythrocyte surface antigen and application thereof
  • Mineralization reagent for shielding erythrocyte surface antigen and application thereof
  • Mineralization reagent for shielding erythrocyte surface antigen and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A method for shielding red blood cell surface antigens, comprising the steps of:

[0043] (1) Mix TMOS with 0.9% NaCl aqueous solution as a mineralization solution, wherein the concentration of TMOS in the mineralization solution is 10 mM;

[0044] (2) will 1×10 9 Individual / mL human erythrocytes (red blood cell blood types are A type, B type, Rh + type) suspension and the mineralization solution of step (1) at a volume ratio (mL:mL) of 1:200, incubated at 4°C, pH=4 for 2h, centrifuged at 3000rpm for 10min and washed with 10mM, pH=7.4 phosphate buffer Wash to remove residual liquid to obtain mineralized erythrocytes; among them, 1×10 9 The preparation method of human erythrocyte suspension per mL is as follows: separate and purify human blood through gradient centrifugation (centrifugation at 3000rpm for 15min, then centrifugation at 3000rpm for 10min), remove white blood cells, platelets and plasma components, and then use 10mM, pH=7.4 Wash repeatedly with PBS buffe...

Embodiment 2

[0047] A method for shielding red blood cell surface antigens, comprising the steps of:

[0048] (1) Mix TEOS with 0.9% NaCl aqueous solution as a mineralization solution, wherein the concentration of TEOS in the mineralization solution is 5 mM;

[0049] (2) will 1×10 9 Each / mL Babl / c mouse erythrocyte suspension was incubated with the mineralization solution of step (1) at a volume ratio (mL:mL) of 1:400 at 4°C and pH=5 for 2h, centrifuged at 3000rpm for 15min and washed with 10mM , pH=7.4 phosphate buffer solution to wash, remove residual liquid, and obtain mineralized erythrocytes; wherein, 1×10 9 The preparation method of the mouse erythrocyte suspension of each / mL is as follows: the blood of the mouse (purchased from the Experimental Animal Center of South China University of Technology) is separated and purified by gradient centrifugation (centrifugation at 3000rpm for 15min, and then at 3000rpm for 10min), and white blood cells are removed. Platelets and plasma compon...

Embodiment 3

[0052] A method for shielding red blood cell surface antigens, comprising the steps of:

[0053] (1) Mix tetraethyl titanate with 0.9% NaCl aqueous solution as a mineralization solution, wherein the concentration of tetraethyl titanate in the mineralization solution is 5 mM;

[0054] (2) will 1×10 9 Each / mL New Zealand rabbit erythrocyte suspension and the mineralization solution of step (1) were incubated at 4°C and pH=4.5 for 1h at a volume ratio (mL:mL) of 1:500, centrifuged at 3000rpm for 10min and washed with 10mM, pH=4.5 7.4 Wash with phosphate buffer to remove residual liquid to obtain mineralized red blood cells; among them, 1×10 9 The preparation method of New Zealand rabbit erythrocyte suspension per mL is as follows: the blood of New Zealand rabbit (purchased from the Experimental Animal Center of South China University of Technology) is separated and purified by gradient centrifugation (centrifugation at 3000rpm for 15min, and then at 3000rpm for 10min), and white...

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Abstract

The invention discloses a mineralization reagent for shielding erythrocyte surface antigens and application thereof, and belongs to the field of hemocyte modification. The mineralization reagent for shielding the erythrocyte surface antigen is a reagent for forming silicon dioxide and / or titanium dioxide under the catalysis of erythrocyte surface amino. According to the method for shielding the red blood cell surface antigen, a silicon dioxide or titanium dioxide nano shielding layer formed by biomineralization of the mineralizing liquid and the red blood cells provides a protection environment for the red blood cells, and the binding of the red blood cell antigen and an antibody in blood is effectively reduced, so that the immunogenicity is reduced; the in-vivo circulation time of red blood cells is not influenced.

Description

technical field [0001] The invention relates to the field of blood cell transformation, in particular to a mineralization reagent for shielding red blood cell surface antigens and its application. Background technique [0002] In modern medicine, blood transfusion is an important medical method. However, related blood products are facing some problems, such as: insufficient and uneven distribution of blood resources; blood products have the risk of virus infection; there are restrictions on transfusion of different blood types. Developing artificial blood is an effective strategy to address the above limitations. In fact, the artificial blood we are talking about today is far from being able to replace whole blood. Instead, we start with red blood cells, the carrier of oxygen transport, to replace them. Usually, people simply divide blood types into A, B, AB, or O. This ABO blood group system actually divides blood types based on the antigens on the surface of red blood c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/078
CPCC12N5/0641C12N5/0006
Inventor 朱伟雷川怡
Owner SOUTH CHINA UNIV OF TECH
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