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Double-enzyme active cobalt monatomic nano-enzyme as well as preparation method and application thereof

A technology of enzyme activity and nano-enzyme, applied in the field of biocatalysts, can solve the problems of reducing anti-tumor effect, accelerating, and consuming active oxygen, and achieve the effect of enhancing active oxygen level, wide sources, and inhibiting growth

Pending Publication Date: 2022-04-19
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, ascorbic acid is used to enhance the anti-tumor effect, but the slow oxidation of ascorbic acid produces less active oxygen, and the intracellular antioxidant (glutathione) will consume active oxygen, which will greatly reduce the anti-tumor effect
In the related art, these two pathways (accelerating AA to produce reactive oxygen species and consuming intracellular GSH to regulate intracellular redox balance) are rarely used to improve the effect of anti-tumor therapy.

Method used

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  • Double-enzyme active cobalt monatomic nano-enzyme as well as preparation method and application thereof
  • Double-enzyme active cobalt monatomic nano-enzyme as well as preparation method and application thereof
  • Double-enzyme active cobalt monatomic nano-enzyme as well as preparation method and application thereof

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Embodiment 1

[0040] The preparation method of Co-N / C SACs catalyst comprises the steps:

[0041] a, the synthesis of Co-ZIF-8:

[0042] At room temperature, 2.76g of Zn(NO 3 ) 2 ·6H 2 O, 0.1723g of Co(NO 3 ) 2 ·6H 2 O mixed metal salts and 6.52 g of 2-methylimidazole were dissolved in 100 mL of methanol solution, and then, the two solutions were quickly mixed and vigorously stirred at 1000 rpm for 1 hour at room temperature. The precipitate was collected by centrifugation, washed three times with methanol, and finally dried at 60 °C to obtain Co-ZIF-8.

[0043] b. Synthesis of Co-N / C SACs

[0044] The prepared precursor Co-ZIF-8 was heated at 2°C min -1 Heated to 900 °C at a heating rate of 900 °C and kept under nitrogen atmosphere at 900 °C for 2 h. The product was obtained at 80 °C in 0.5 M H 2 SO 4 The solution was stirred for 8h to remove residual zinc. After being collected by centrifugation, washed with water and ethanol six times, and finally dried under vacuum at 60 °C,...

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Abstract

The invention relates to a double-enzyme active cobalt monatomic nano-enzyme as well as a preparation method and application thereof. The double-enzyme active cobalt monatomic nano-enzyme comprises a carbon skeleton and Co monatomic anchored on the carbon skeleton, the double-enzyme active cobalt monatomic nano-enzyme is prepared by a method comprising the following steps: (1) Zn (NO3) 2.6 H2O, Co (NO3) 2.6 H2O and 2-methylimidazole are subjected to a reaction to synthesize Co-ZIF-8; and (2) carrying out pyrolysis on the Co-ZIF-8, and treating with H2SO4 to obtain the Co-ZIF-8. The Co-N / C SACs catalyst is of a monatomic structure, has double-enzyme activity and can simulate the activity of ascorbic acid oxidase and glutathione oxidase at the same time.

Description

technical field [0001] The invention belongs to the technical field of biocatalysts, and in particular relates to a dual-enzyme active cobalt single-atom nanozyme and a preparation method and application thereof. Background technique [0002] Ascorbic acid (AA, also known as vitamin C) has also been recognized as an effective treatment for cancer since the 1970s. Clinical trials have shown that AA is a potential adjuvant that can improve the efficacy of cancer treatments including ovarian cancer, brain cancer, and lung cancer. Most researchers believe that the core pharmacological mechanism of AA's anticancer effect is the generation of hydrogen peroxide (H 2 o 2 ) to inhibit tumor growth. Previous studies have shown that H 2 o 2 Selectively induces oxidative stress in cancer cells because alterations in active iron metabolism in cancer cells make them more sensitive to changes in ascorbate levels compared to normal cells. However, the autooxidation of ascorbic acid is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J27/24B01J23/75B01J37/08B82Y30/00B82Y40/00A61K31/375A61K33/24A61P35/00
CPCB01J27/24B01J23/75B01J37/086B82Y30/00B82Y40/00A61K31/375A61K33/24A61P35/00A61K2300/00
Inventor 林雨青任国圆卢明菊者亚东李凯
Owner CAPITAL NORMAL UNIVERSITY
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