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Dunaliella salina strain for treating salt-containing wastewater, microbial community comprising Dunaliella salina strain and application of microbial community

A microbial community, Dunaliella salina technology, applied in the field of Dunaliella salina algae strains, can solve problems such as reducing COD removal efficiency, and achieve cost-effective effects

Pending Publication Date: 2022-04-12
山东海之宝海洋科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous research results have shown that high salinity (~6%) will significantly reduce the removal efficiency of COD (Uygur and Kargi, Salt inhibition on biological nutrient removal from saline wastewater in a sequencing batchreactor. Enzym. Microb. Technol., 34 (3 –4), pp.313-318(2004))

Method used

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  • Dunaliella salina strain for treating salt-containing wastewater, microbial community comprising Dunaliella salina strain and application of microbial community
  • Dunaliella salina strain for treating salt-containing wastewater, microbial community comprising Dunaliella salina strain and application of microbial community
  • Dunaliella salina strain for treating salt-containing wastewater, microbial community comprising Dunaliella salina strain and application of microbial community

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0151] Example 1. Isolation, purification and identification of Dunaliella salina and symbiotic bacteria

[0152] Isolation and purification of Dunaliella salina: The salinity of the kelp production and processing water sampled by Haizhibao Company and brought back to Jacobs University Bremen is close to 20%. After adding twice the f / 2 (Guillard 1975) medium nutrient salt to the sampled processing water, place it in a temperature-controlled room at 21±1°C for cultivation. After one month of cultivation, the concentration of microalgae cells in the sample increased significantly compared with the initial colorless water sample, as shown in figure 1 As shown in A. Spread an integer volume of algae liquid evenly on an agar plate supplemented with twice the f / 2 nutrient salt and cultivate until the microalgae single colony of Dunaliella salina grows on the plate, such as figure 1 Shown in B. Single colonies of microalgae are picked and transferred to separate culture flasks to ...

Embodiment 2

[0164] Embodiment 2: Batch cultivation of Dunaliella salina in universal medium with different salinities

[0165] Although Dunaliella salina is characterized by tolerance to high salinity, salinity may have an impact on wastewater treatment efficiency and carotenoid accumulation. Therefore, it is necessary to optimize the salinity of process water before using Dunaliella salina to treat process water. For this reason, in this example, the optimum salinity suitable for the processing water of Dunaliella salina was tested.

[0166] The culture conditions are as follows: use 4 times of f / 2 universal medium to cultivate Dunaliella salina, where: initial OD 725 The value is 0.2; the salinity of the medium is adjusted by adding sodium chloride; the illumination is provided by a fluorescent tube: the light intensity is 200 μmol / m 2 / s; the light cycle is 14 hours light / 10 hours dark; the room temperature is maintained at 25±1°C; the pH is set to 7.4 and the hysteresis effect of 0....

Embodiment 3

[0171] Example 3: Cultivation of Dunaliella salina in synthetic process water

[0172] According to the analysis report of the original saturated processing water provided by Haizhibao, the synthetic processing water is prepared to simulate the actual wastewater treatment situation. According to the information provided by Haizhibao, the water source for processing water is seawater. Therefore, in this experiment, in order to better simulate the actual water source that needs to be treated, North Sea seawater was used to prepare synthetic processing water in the laboratory. By adding sodium chloride To increase the salinity of seawater, add NH 4 Cl and KH 2 PO 4 as inorganic nutrients. The contents of nutrients and total organic carbon (TOC) in the prepared processing water with a salinity of 5%, 10% and 15% are shown in Table 2 below. Of course, it is understood that in order to achieve maximum nutrient recovery efficiency and biomass yield, the saturated process water ca...

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Abstract

The present invention relates to a Dunaliella sp. Strain capable of recovering inorganic nutrients from high salinity processing wastewater, and a microbial community comprising the strain and a symbiotic bacterium thereof. The symbiotic bacteria include halomonas, marine bacteria, or a combination thereof. The invention further relates to application of the dunaliella salina strain and the microbial community containing the dunaliella salina strain to treatment of high-salinity salt-containing wastewater such as kelp processing water, production of high-value-added photosynthetic pigments including chlorophyll and carotenoid and biological carbon sequestration.

Description

technical field [0001] The invention relates to the field of environmental microorganisms. Specifically, the present invention relates to a Dunaliella sp. algae strain for treating high-salinity wastewater, including the algae strain and its symbiotic bacterial microbial community, and their use. Background technique [0002] Saline wastewater is produced in the production process of various industries such as chemical industry, agriculture, textile industry and leather industry (Lefebvre and Moletta, Treatment of organic pollution in industrial saline wastewater: a literature review. Water Res., 40, pp.3671 -3682(2006)), saline wastewater contains a variety of organic pollutants. Taking the processing water in the production process of kelp as an example, the preservation of kelp requires the use of a large amount of salt (mainly sodium chloride) to prolong the shelf life of kelp, so a large amount of high-salinity processing water is produced during the rinsing process of...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/20C12P39/00C12P23/00C02F3/34C02F3/32C12R1/89C12R1/01
Inventor 刘晓勇王松L·汤姆森
Owner 山东海之宝海洋科技有限公司
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