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Antibacterial antioxidant degradable preservative film and preparation method thereof

A fresh-keeping film and anti-oxidation technology, applied in the field of fresh-keeping film, can solve the problems of weakened antibacterial agent, side effects, and inconvenient use of bacteriophage, and achieve the effects of reducing loss rate, extending shelf life, and improving stability and activity

Active Publication Date: 2022-04-05
SICHUAN ACAD OF FOOD & FERMENTATION INDS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems in the existing technical schemes that drug-resistant bacterial strains cause the effect of antibacterial agents to weaken day by day, conventional fresh-keeping techniques are not convenient enough to use phages, have side effects, and need to consider environmental protection, the present invention provides an antibacterial, anti-oxidative and degradable Preservative film and preparation method thereof

Method used

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  • Antibacterial antioxidant degradable preservative film and preparation method thereof
  • Antibacterial antioxidant degradable preservative film and preparation method thereof
  • Antibacterial antioxidant degradable preservative film and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The invention provides a preparation method of an antibacterial and antioxidative degradable plastic wrap, comprising the following steps:

[0029] S1, preparation of phage suspension

[0030] The phage stock solution was inoculated and cultured in proportion to its host bacterium Escherichia coli O157:H7, and then centrifuged and filtered to obtain a phage suspension, wherein the phage titer was 10 Log PFU / mL.

[0031] S2, preparation of gelatin solution

[0032] 5 wt% fish gelatin was added to distilled water, heated at 50 °C for 30 min, after the fish gelatin was dissolved, 0.2 wt% glycerin was added as a plasticizer, and stirred for 5 min to fully cross-link the fish gelatin and glycerin, and the obtained Gelatin film-forming base fluid.

[0033] S3, film making

[0034] Add 5% phage suspension and 0.1 wt% protocatechuic acid (PCA) to the gelatin film-forming base solution, then stir, ultrasonically defoam, and then pour it on a smooth polypropylene plate to form...

Embodiment 2

[0036] The phage stock solution was inoculated and cultured in proportion to its host bacterium Escherichia coli O157:H7, and then centrifuged and filtered to obtain a phage suspension, wherein the phage titer was 10 Log PFU / mL.

[0037] S2, preparation of gelatin solution

[0038] Add 1.5 wt% fish gelatin in distilled water, heat at 60 °C for 30 min, after the fish gelatin dissolves, add 0.2 wt% glycerin as a plasticizer, stir for 5 min to fully cross-link the fish gelatin and glycerin, and obtain Gelatin film-forming base fluid.

[0039] S3, film making

[0040] Add 1% phage suspension and 0.1 wt% protocatechuic acid to the gelatin film-forming base solution, then stir, ultrasonically defoam, and then pour it on a smooth polypropylene plate to form a film, after a low-temperature After drying, peeling and cutting, an antibacterial and anti-oxidation degradable gelatin-phage-PCA plastic wrap containing phage and protocatechuic acid is prepared.

Embodiment 3

[0042] The invention provides a preparation method of an antibacterial and antioxidative degradable plastic wrap, comprising the following steps:

[0043] S1, preparation of phage suspension

[0044] The phage stock solution was inoculated and cultured in proportion to its host bacterium Escherichia coli O157:H7, and then centrifuged and filtered to obtain a phage suspension, wherein the phage titer was 10 Log PFU / mL.

[0045] S2, preparation of gelatin solution

[0046] 5 wt% fish gelatin was added to distilled water, heated at 70 °C for 50 min, after the fish gelatin was dissolved, 0.6 wt% glycerin was added as a plasticizer, and stirred for 5 min to fully cross-link the fish gelatin and glycerin, and the obtained Gelatin film-forming base fluid.

[0047] S3, film making

[0048] Add 5% phage suspension and 0.2 wt% protocatechuic acid to the gelatin film-forming base solution, then stir, ultrasonically defoam, and then pour it on a smooth polypropylene plate to form a fil...

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Abstract

The invention discloses an antibacterial antioxidant degradable preservative film and a preparation method thereof, and relates to the technical field of preservative films, the adopted technical scheme comprises the following steps: S1, inoculating and culturing a bacteriophage stock solution and host bacteria, and then preparing a bacteriophage suspension; s2, dissolving gelatin, adding a plasticizer, and uniformly stirring to obtain a gelatin film-forming base solution; and S3, adding the bacteriophage suspension and 0.1-0.2 wt% of protocatechuic acid into the gelatin film forming base solution, and forming a film, so as to prepare the gelatin-bacteriophage-PCA preservative film. The preservative has more effective antibacterial and antioxidant properties, the protocatechuic acid enhances the storage stability and release efficiency of the bacteriophage, the number of targeted food-borne pathogenic bacteria can be effectively reduced, and when the preservative is applied to preservation of fresh food such as meat and fresh vegetables, the shelf life of goods can be prolonged.

Description

technical field [0001] The invention relates to the technical field of fresh-keeping film, in particular to an antibacterial and anti-oxidation degradable fresh-keeping film and a preparation method thereof. Background technique [0002] Microbial metabolism is an important cause of food spoilage, especially foodborne diseases caused by contamination of pathogenic bacteria, which seriously threaten human health and even life. Common food-borne pathogens include Escherichia coli, Salmonella, Listeria monocytogenes, Vibrio parahaemolyticus, etc. Escherichia coli O157:H7 is a very representative one, and livestock and their meat products are Escherichia coli The important transmission route of O157:H7, especially in beef and its products, is estimated to cause an annual loss of 250 million US dollars. [0003] Due to the serious environmental pollution caused by the extensive use of non-degradable food packaging materials in recent years, the research on functional food packag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J5/18C08L89/00C08K5/13C08K5/00
CPCY02W90/10
Inventor 后鹏飞黄静罗丹唐人杰陈功游敬刚
Owner SICHUAN ACAD OF FOOD & FERMENTATION INDS
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