Cardiovascular implant based on in-situ regulation immune response and preparation method thereof
An immune response and cardiovascular technology, which is applied in the field of biomedicine, can solve problems such as neural network reconstruction with little attention, and achieve high collagen safety and biocompatibility, extended validity period, and good biocompatibility.
Active Publication Date: 2022-03-29
ARMY MEDICAL UNIV
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Problems solved by technology
[0003] However, the current research on organ transplantation mainly focuses on allograft rejection and functional reconstruction, but little attention is paid to neural network reconstruction.
Method used
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Embodiment
[0041] Embodiment: A kind of preparation method of cardiovascular implant based on in situ regulation immune response, such as figure 1 , figure 2 , image 3 shown, including the following steps:
[0042] (1) Build a cardiovascular implant body:
[0043] 1) Under sterile conditions, extract the common carotid artery from 250-300g SD rats, wash the blood with normal saline, separate and remove the outer connective tissue of the common carotid artery, and then cut it into small pieces of blood vessels with a length of 0.5-1cm (generally 0.5-1cm in length) Tissue engineered blood vessels of 0.5-20 cm can meet the needs of clinical applications such as blood vessel transplantation, and the transplanted objects of the cardiovascular implants are rats, so small blood vessels with a length of 0.5-1 cm are suitable).
[0044] Trypsin was diluted with M199 medium to a concentration of 0.05%, and blood vessels were digested at 37°C for 30 minutes to remove cells, and then nucleic ac...
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Abstract
The invention discloses a cardiovascular implant based on in-situ regulation and control of immune response and a preparation method thereof, and belongs to the technical field of biological medicine, and the technical scheme is characterized in that the cardiovascular implant comprises a cardiovascular implant body and H4000-CD25/dcas9 slow-release nanoparticles modified on the cardiovascular implant body; the H4000-CD25/dcas9 sustained-release nano particle comprises an H4000 plasmid nano carrier (Engreen), a CD25 antibody and a dcas9 plasmid sequence, and is characterized in that the H4000-CD25/dcas9 sustained-release nano particle comprises a H4000 plasmid nano carrier (Engreen), the preparation method of the cardiovascular implant comprises the following steps: constructing the cardiovascular implant body, preparing the H4000-CD25 nano transfection carrier, preparing the H4000-CD25/dcas9 sustained-release nano-particles, and coupling the H4000-CD25/dcas9 sustained-release nano-particles to the cardiovascular implant body. The method is mainly used for constructing the cardiovascular implant modified with the H4000-CD25/dcas9 sustained-release nanoparticles, nerve fibers can be induced to grow into engineering blood vessels, and the anti-thrombus function of the cardiovascular implant is improved and in-situ regeneration of the cardiovascular implant is promoted by utilizing the regulation and control capability of Treg cells on immune response.
Description
technical field [0001] The invention relates to the technical field of biomedicine, in particular to a cardiovascular implant based on in situ regulation of immune response and a preparation method thereof. Background technique [0002] Cardiovascular disease is the number one disease that threatens human health. Every year 7.3 million people die from ischemic heart disease worldwide, ranking first among all diseases. Therefore, the demand for cardiovascular implants is increasing. Among them, bioartificial blood vessels are the development direction of vascular implants for coronary artery bypass grafting, hemodialysis and peripheral vascular occlusion. Moreover, the construction of complex tissues and organs such as liver, kidney, lung, and islets of pancreas also requires vascularization, leading to a further increase in the demand for bioartificial blood vessels. Human beings are a whole, and the functioning of a certain organ in one system often requires the interactio...
Claims
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IPC IPC(8): A61L27/36A61L27/22A61L27/34A61L27/50A61L27/54C12N5/10C12N15/90C12N15/85C12N15/13C12N15/113C12N15/55
CPCA61L27/3625A61L27/34A61L27/507A61L27/227A61L27/54A61L27/3604C12N15/907C12N15/85C12N9/22C12N15/113C07K16/2866A61L2300/624A61L2300/41A61L2300/258A61L2300/256C12N2310/20C08L89/00A61L2300/254A61L2300/602A61L2430/20A61L2400/12A61L2300/252A61K47/6957A61K47/6931A61K47/6849
Inventor 曾文李彦朝汪艳宏薛方超
Owner ARMY MEDICAL UNIV
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