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Flory polyglycoside anabolism combined gene and combined enzyme

A technology of synthesizing metabolism and combining enzymes, which is applied in the field of genetic engineering and can solve problems such as less research

Pending Publication Date: 2022-03-18
OCEAN UNIV OF CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, there are few studies on the anabolic mechanism of fucoside and the enzymes that play a key role in anabolism.

Method used

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  • Flory polyglycoside anabolism combined gene and combined enzyme
  • Flory polyglycoside anabolism combined gene and combined enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The sample is the fronds of wild altar Porphyra collected in Dongjia Island, Fujian Province on December 11, 2019. The Porphyra altar was transferred to an indoor aerated culture system, and the PES medium (Provasoli, L., 1968) was used to recover the culture at 21°C for 48 hours in the dark. After resuming the culture, put Porphyra altar at 21°C, 50mol·m -2 the s -1 Under the condition of light (12h light, 12h dark), culture medium was changed every 2 days. On the 5th day of cultivation, when the light was 6 hours, Porphyra altar with almost the same size was selected for gene analysis and content determination.

Embodiment 2

[0036] Example 2 Research on Floridoside Synthesis and Metabolism Pathway

[0037] Synthetic and metabolic pathways of floridoside in laver figure 1 As shown, fructose-6-phosphate (Fru-6-P) is converted by phosphoglucose isomerase (PhPGI) to glucose-6-phosphate (Glu-6-P), which is then converted by glucose phosphomutase (PhPGM) Glucose-1-phosphate (Glu-1-P). Under the catalysis of UDPG pyrophosphorylase (PhUGP), Glu-1-P reacts with UTP to generate UDP-glucose (UDP-Glu). UDP-glucose is isomerized to UDP-galactose (UDP-Gal) under the catalysis of UDP-glucose 4-isomerase (PhGALE). Then UDP-galactose and glycerol-3-phosphate (Gly-3-P) synthesize floridoside under the catalysis of trehalose phosphate synthase (PhTPS) and trehalose phosphate phosphorylase (PhTPP).

Embodiment 3

[0038] Example 3 Gene Analysis

[0039] (1) From NCBI (https: / / www.ncbi.nlm.nih.gov / ), MGU

[0040] (https: / / marinegenomics.oist.jp / algae / gallery) and Ensembl

[0041] (http: / / plants.ensembl.org / index.html) Download the homologous sequences involved in the floridoside metabolic pathway from the database, and save them in fasta format;

[0042] (2) Utilize Secure FX and Putty software to connect to the Porphyra genome database on the main server, and run the command "formatdb-iOneKP.faspF" in Putty to format the database;

[0043] (3) Use Secure FX to upload the protein sequence file downloaded to the local computer to the formatted database folder;

[0044] (4) Use the "blastall-p tblastn-d(sequence name)-i(searchsequence)-o out-(gene name)-FF-e 1e-5" command in Putty to retrieve the sequence with amino acid as query, The result file is opened in .txt format and saved to the local computer;

[0045] (5) Use Editplus (https: / / www.editplus.com / ), perl and Tbtools software to...

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Abstract

The invention provides a floridin anabolism combined gene and a combined enzyme, and belongs to the field of gene engineering. According to the present invention, through the research on the floridin anabolism mechanism in porphyra haitanensis, two kinds of glucose phosphate isomerase (PhGPI), three kinds of glucose phosphate mutase (PhPGM), two kinds of UDPG pyrophosphorylase (PhUGP), one kind of UDP-glucose 4-isomerase (PhGALE), three kinds of trehalose phosphate synthetase (PhTPS) and two kinds of trehalose phosphate phosphorylase (PhTPP) are found, and the new floridin anabolism process is provided; and a theoretical basis is provided for researching the anabolism process of floridoside and the growth and development of red algae.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a floripolyside synthetic metabolism combined gene and combined enzyme. Background technique [0002] Altar seaweed (Pyropia haitanensis), commonly known as seaweed and Wucai, is a large seaweed that can be cultivated artificially. Altar Porphyra belongs to Rhodophyceae, Porphyridaceae, Porphyra genus, dark purple green slightly brown algae, lanceolate, subovate or long ovate, more than 12-30cm long, heart-shaped, round or wedge-shaped at the base , slightly wrinkled or absent at the edge, with sparse sawtooth, single layer of algal body, partial double layer, single or few double chromatophores, round-capitate cells at the base, dioecious, few monogenic, warm temperate species, It is the main cultivated algae along the coasts of Zhejiang, Fujian and Guangdong in China. Rich in protein, polysaccharides and vitamins, it can be used for food or medicine. [0003] D...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/61C12N15/54C12N9/92C12N9/90C12N9/12C12N9/10C12P19/44
CPCC12N9/92C12N9/90C12N9/1241C12N9/1051C12N15/52C12P19/44C12Y503/01009C12Y504/02002C12Y207/07009C12Y501/03002C12Y204/01015C12Y204/01036C12Y204/01216
Inventor 于亚慧刘涛贾旭利
Owner OCEAN UNIV OF CHINA
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