Method for extracting nostoc sphaeroides polysaccharide

An extraction method and solution technology, which is applied in the field of extracting kudzu rice polysaccharides, can solve the problems of low purity and yield, time-consuming and laborious, etc., and achieve the effects of high yield, wide application prospects, and reduced dosage

Active Publication Date: 2022-03-15
HUNAN YANDI BIOLOGICAL ENG
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  • Abstract
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Problems solved by technology

The polysaccharides in Gexianmi are rich in polysaccharides and have a variety of physiological activities, which have attracted great attention from the medical and food science circles. Mo Kaiju found that the antioxidant activity of wild Gexianmi polysaccharides is weak, but it can reduce the thymus index and inhibit the growth of cells caused by tumors. Decreased immunity and no toxicity to kidney tissue; Peng Changan et al. found by MTT colorimetry that the polysaccharides of Gexianmi had good inhibitory ability to the proliferation of HCT-116 cells; Chang Xiangdong et al. used filter paper patch method to study Gexianmi Water-soluble polysaccharides have inhibitory effects on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and mold; Zhu Yuting et al. found that the sulfated kudzu rice polysaccharide has anticoagulant function, but the current extraction method of kudzu rice polysaccharide Time-consuming and labor-intensive, and the purity and yield are low

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  • Method for extracting nostoc sphaeroides polysaccharide

Examples

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Effect test

Embodiment 1

[0023] A kind of extracting method of kudzu rice polysaccharide:

[0024] Dry 100g of kudzu rice to constant weight and then pulverize it to get kudzu rice powder, soak it in 0.5mol / L sodium hydroxide solution at 45°C for 4h, then filter out, the ratio of solid to liquid is 1:120, and then transfer to steam In the blasting equipment, after sealing, pass saturated steam until the pressure in the steam blasting equipment reaches 8MPa. After holding the pressure for 15 minutes, open the valve to release the pressure. Collect the obtained solid and add it to the disodium hydrogen phosphate-citric acid buffer solution. After stirring for 30 minutes, add the A compound enzyme preparation composed of cellulase, papain, and neutral protease in a weight ratio of 2:3:1. The dosage of the compound enzyme preparation is 1% of the weight of Ge Xianmi powder, and the temperature is raised to 60°C for 12 hours. After the end of the enzymolysis The solution was heated to reflux for 10 hours a...

Embodiment 2

[0026] A kind of extracting method of kudzu rice polysaccharide:

[0027] Dry 100g of kudzu rice to constant weight and then pulverize it to get kudzu rice powder, soak it in 0.6mol / L sodium hydroxide solution at 45°C for 4h, then filter out, the ratio of solid to liquid is 1:120, and then transfer to steam In the blasting equipment, after sealing, pass saturated steam until the pressure in the steam blasting equipment reaches 8MPa. After holding the pressure for 15 minutes, open the valve to release the pressure. Collect the obtained solid and add it to the disodium hydrogen phosphate-citric acid buffer solution. After stirring for 40 minutes, add the A compound enzyme preparation composed of cellulase, papain, and neutral protease in a weight ratio of 2:3:1. The dosage of the compound enzyme preparation is 1% of the weight of Ge Xianmi powder, and the temperature is raised to 65°C for 12 hours. After the end of the enzymolysis The solution was heated to reflux for 10 hours a...

Embodiment 3

[0029] A kind of extracting method of kudzu rice polysaccharide:

[0030] Dry 100g of kudzu rice to constant weight and then pulverize it to get kudzu rice powder, soak it in 0.2mol / L sodium hydroxide solution at 40°C for 2h, then filter out, the ratio of solid to liquid is 1:80, and then transfer to steam In the blasting equipment, after sealing, pass saturated steam until the pressure in the steam blasting equipment reaches 6MPa. After holding the pressure for 10 minutes, open the valve to release the pressure. Collect the obtained solid and add it to the disodium hydrogen phosphate-citric acid buffer solution. After stirring for 20 minutes, add the A compound enzyme preparation composed of cellulase, papain, and neutral protease in a weight ratio of 2:3:1. The dosage of the compound enzyme preparation is 0.5% of the weight of Ge Xianmi powder, and the temperature is raised to 55°C for 8 hours. After the end of the enzymolysis The solution was heated to reflux for 5 hours an...

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Abstract

The invention relates to the technical field of extraction, in particular to a nostoc sphaeroids kutz polysaccharide extraction method, which comprises the following steps: drying nostoc sphaeroids kutz to constant weight, crushing to obtain nostoc sphaeroids kutz powder, adding the nostoc sphaeroids kutz powder into a sodium hydroxide solution at 40-45 DEG C, soaking, filtering out, carrying out steam explosion treatment, adding into a disodium hydrogen phosphate-citric acid buffer solution, adding a compound enzyme preparation, and uniformly stirring to obtain the nostoc sphaeroids kutz polysaccharide. The purity of the nostoc sphaeroids kutz polysaccharide prepared through the method reaches 97% or above, the yield is high, and the nostoc sphaeroids kutz polysaccharide has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of extraction, in particular to a method for extracting kudzu rice polysaccharide. Background technique [0002] Gexianmi is a low-level single-celled cyanobacteria of the cyanophyta Nostocaceae. The ancient name Tianxiancai and Tianxianmi are commonly known as water fungus. According to "Compendium Supplements", it is recorded that Gexianmi "is cold in nature and sweet in taste" and has the effects of "antipyretic, clearing the diaphragm and benefiting the stomach", and is a rare natural pollution-free green food. The polysaccharides in Gexianmi are rich in polysaccharides and have a variety of physiological activities, which have attracted great attention from the medical and food science circles. Mo Kaiju found that the antioxidant activity of wild Gexianmi polysaccharides is weak, but it can reduce the thymus index and inhibit the growth of cells caused by tumors. Decreased immunity and no toxicity to k...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 王翠霞余佳唐丹王灿郑小娟付夏娜张瑞华
Owner HUNAN YANDI BIOLOGICAL ENG
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