Cultivation method and development and application of eucommia triploid new variety
A cultivation method and triploid technology are applied in the cultivation method of new eucommia triploid varieties and the development and application fields thereof, which can solve the problems of low yield and restriction of triploid eucommia ulmoides, improve the induction success rate, avoid damage, Yield-enhancing effect
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Embodiment 1
[0034] The present embodiment provides a method for cultivating a new triploid variety of Eucommia ulmoides, comprising the following steps:
[0035] a: Induction of 2n female gametes: 0.05wt% colchicine solution and 40°C high temperature treatment were applied to the female flower buds of Eucommia ulmoides female plants.
[0036] Specifically, the female flower buds are induced on the first day after the male plants of the same age start to loosen the powder. The colchicine treatment method is: drop the colchicine solution on the female flower buds until the flower buds are completely soaked, and add dropwise every half an hour. The time from the first drop to the last drop is 24 hours.
[0037] Begin to carry out high-temperature treatment while colchicine is processed, adopt tree non-isolated branch bud heating treatment device (authorization announcement number CN101057555B) to carry out, and high-temperature treatment time is 9 hours.
[0038] b: Artificial pollination: ...
Embodiment 2
[0044] The present embodiment provides a method for cultivating a new triploid variety of Eucommia ulmoides, comprising the following steps:
[0045] a: Induction of 2n female gametes: 0.25wt% colchicine solution and 46°C high temperature treatment were applied to female flower buds of Eucommia ulmoides female plants.
[0046] Specifically, the female flower buds are induced on the first day after the male plants of the same age start to loosen the powder. The colchicine treatment method is: drop the colchicine solution on the female flower buds until the flower buds are completely soaked, and add dropwise every half an hour. The time from the first drop to the last drop is 24 hours.
[0047] Begin to carry out high-temperature treatment while colchicine is processed, adopt tree non-isolated branch bud heating treatment device (authorization announcement number CN101057555B) to carry out, and high-temperature treatment time is 9 hours.
[0048] b: Artificial pollination: sele...
Embodiment 3
[0054] The present embodiment provides a method for cultivating a new triploid variety of Eucommia ulmoides, comprising the following steps:
[0055] a: Induction of 2n female gametes: 0.5wt% colchicine solution and 50°C high temperature treatment were applied to female flower buds of Eucommia ulmoides female plants.
[0056] Specifically, the female flower buds are induced on the first day after the male plants of the same age start to loosen the powder. The colchicine treatment method is: drop the colchicine solution on the female flower buds until the flower buds are completely soaked, and add dropwise every half an hour. The time from the first drop to the last drop is 24 hours.
[0057] Begin to carry out high-temperature treatment while colchicine is processed, adopt tree non-isolated branch bud heating treatment device (authorization announcement number CN101057555B) to carry out, and high-temperature treatment time is 9 hours.
[0058] b: Artificial pollination: selec...
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