Detection method of ceftiofur and application thereof

A detection method, the technology of ceftiofur, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unsuitable rapid detection of ceftiofur, many processing steps, and high toxicity, and achieve low toxicity, simple pretreatment, The effect of using less reagent

Pending Publication Date: 2022-03-01
TIANJIN RINGPU BIO TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above treatment methods for ceftiofur in samples all use iodoacetamide solution derivatization and solid-phase extraction column treatment, there are many sample pretreatment steps, high cost, long time, many reagents required for sample pretreatment, and high toxicity and other shortcomings, it is not suitable for the rapid detection of ceftiofur and its metabolites in large batches of plasma samples in clinical pharmacokinetic tests

Method used

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  • Detection method of ceftiofur and application thereof
  • Detection method of ceftiofur and application thereof
  • Detection method of ceftiofur and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The detection method of ceftiofur in pig plasma in embodiment 1

[0042] 1. Preparation of the main solution

[0043] 0.05 mol / L boric acid buffer solution: Weigh 19 g boric acid and 3.7 g potassium chloride, add water to dissolve and dilute to 1000ml.

[0044] Extract (0.4% DTE w / v): Weigh 1.0 g of dithioerythritol (DTE), dissolve it in 250 ml of borate buffer, and prepare immediately after use.

[0045] 0.1% trifluoroacetic acid-water solution: Take 1 ml trifluoroacetic acid and add it to 1000 ml ultrapure water, mix well.

[0046] 1 mg / ml DFC standard stock solution: Weigh about 10 mg of DFC standard into a 10 ml brown volumetric flask, dissolve with methanol and dilute to volume, store at -70°C for later use.

[0047] 2. Sample pretreatment

[0048] Take 200 μl of plasma sample, add 400 μl of extract solution (0.4% DTE w / v), vortex mix, and 50°C water bath for 30 min, and vortex mix once every 10 min. After the water bath was completed, the sample was centrifuge...

Embodiment 2

[0088] Embodiment 2 Pharmacokinetic comparative test of three kinds of ceftiofur injections in pigs

[0089] There were 18 healthy Landrace binary hybrid pigs, weighing 90-110 kg. Before the experiment, the rats were fed for 1 week as usual, and the feed was a diet without any antibiotics. Before the experiment, the pigs used in the experiment were screened, and the age, weight, and fat condition were as consistent as possible. Using a single-center, completely randomized, single-period parallel, single-administration experimental design, 18 healthy Landrace binary crossbreed pigs were divided into the first, second, and third groups, a total of three groups, with 6 pigs in each group, male and female. Half.

[0090] Fasted for 12 h before administration, but provided adequate drinking water. The next morning on an empty stomach, the pigs in the first, second and third groups were injected intramuscularly at a dose of 5 mg / kg b.w. (calculated as ceftiofur), and the first gr...

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Abstract

The invention provides a method for detecting ceftiofur in plasma. The method for detecting the ceftiofur in the blood plasma comprises the following steps: extracting a ceftiofur metabolite, namely furanformyl ceftiofur (DFC), from a drug-containing blood plasma sample by virtue of an extracting solution-dithioerythritol (DTE) solution in a water bath, without derivatization of an iodoacetamide solution and solid-phase extraction column treatment, centrifuging the extracting solution to obtain a supernatant, and purifying the supernatant by virtue of a chromatographic column, so as to obtain the ceftiofur in the blood plasma. And filtering by using a filter membrane, and directly detecting by using a high performance liquid chromatograph. The method has the advantages that the sample pretreatment steps are simplified, the cost is reduced, the time is saved, the reagent usage amount is small, the toxicity is low, and the method is suitable for rapid detection of ceftiofur and metabolites thereof in a large batch of plasma samples in clinical pharmacokinetic tests.

Description

technical field [0001] The invention belongs to the field of biological sample detection, in particular to the field of detection of ceftiofur and its metabolites, in particular to the detection of ceftiofur metabolites in blood plasma. Background technique [0002] Ceftiofur, also known as Saidefu, is a third-generation cephalosporin antibiotic developed exclusively for veterinary medicine in the United States in the 1980s. The drug was first launched in the United States in 1988. Due to its excellent antibacterial activity and drug Kinetic characteristics, has been officially approved by the United States, Canada, Japan and some European countries for the treatment of respiratory diseases in beef cattle, dairy cows, horses, pigs, sheep. The chemical name of ceftiofur is (6R,7R)-7-[2-(2-aminothiazol-4-yl)(methoxyimino)acetamido]-3-[(2-furylcarbonyl) Thiomethyl]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid, molecular formula C 19 h 17 N 5 o 7 S 3 , molec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062G01N2030/067
Inventor 杨莹莹孟小宾张玉良刘梦月王倩刘爱玲李亚玲尹相吉李守军张钰洁聂丽娜
Owner TIANJIN RINGPU BIO TECH
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