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Dental pulp mesenchymal stem cell culture medium and application method thereof

A kind of stem cell and culture medium technology, applied in the field of stem cells and regenerative medicine, can solve the problems of cytotoxicity and side effects, low cell efficiency, microbial contamination of medium, etc., and achieve the effect of strong cell proliferation promotion and strong antibacterial effect

Active Publication Date: 2022-03-01
GUANGDONG PROCAPZOOM BIOSCIENCES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]1. The current conventional medium for dental pulp mesenchymal stem cells will produce toxic substances with the increase of cell culture time, which will have toxic side effects on cells and cause cell survival low rate
[0005]2. The in vitro culture of dental pulp mesenchymal stem cells takes a long time and takes up a lot of space, and the efficiency of primary culture target cells is low
[0006]3. There are many types of bacteria in the teeth and mouth. During the culture and expansion process of primary dental pulp stem cells, the culture medium is extremely susceptible to microbial contamination

Method used

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  • Dental pulp mesenchymal stem cell culture medium and application method thereof
  • Dental pulp mesenchymal stem cell culture medium and application method thereof
  • Dental pulp mesenchymal stem cell culture medium and application method thereof

Examples

Experimental program
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Embodiment 1

[0038]Dental pulp mesenchymal stem cells were cultured using the dental pulp mesenchymal stem cell culture medium prepared in this example, and the specific steps were as follows:

[0039] Step 1: Add 3mL base glue solution in T25 culture bottle, (base base glue solution consists of containing 1% Matrix’s DMEM / F12 medium), put it into a 37°C incubator and incubate for 30min to obtain a culture bottle covered with base glue;

[0040] Step 2: resuspending the dental pulp mesenchymal stem cells in 5 mL of dental pulp mesenchymal stem cell medium to obtain a medium containing dental pulp mesenchymal stem cells;

[0041] Step 3: Transfer the culture medium containing dental pulp mesenchymal stem cells to a culture bottle covered with base glue, and perform primary culture in an incubator at 37°C;

[0042] Step 4: When the confluence of the primary cultured cells reaches 90%, subculture is performed every 3 days, and the subculture ratio is 1:3.

[0043] Table 1 Contents of each ...

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Abstract

The invention provides a dental pulp mesenchymal stem cell culture medium, which is prepared from the following components in percentage by volume: 5 to 20 percent of platelet lysis buffer, 0.05 to 0.3 percent of epidermal growth factor solution, 0.05 to 0.3 percent of basic fibroblast growth factor solution, 0.1 to 1 percent of L-alanyl-L-glutamine additive and a cell basal culture medium. According to the dental pulp mesenchymal stem cell culture medium, the contents of the components are added and limited, so that the dental pulp mesenchymal stem cells can be promoted to rapidly proliferate from the aspects of supplying cell nutrition, preventing bacterial pollution and promoting cell growth.

Description

technical field [0001] The invention relates to the field of stem cells and regenerative medicine, in particular to a culture medium for dental pulp mesenchymal stem cells and a method for applying the same. Background technique [0002] Mesenchymal stem cells (MSCs) are a kind of adult stem cells that can be cultured and expanded in vitro and have multi-directional differentiation potential. Under specific induction conditions, they can not only differentiate into osteoblasts, chondrocytes, adipose Cells and other mesoderm tissue cells, and can differentiate into endoderm tissue cells and ectoderm tissue cells. Mesenchymal stem cells were first discovered in bone marrow tissue. With the deepening of research, they have also been found in various tissues of the human body, such as dental pulp, umbilical cord, placenta, fat, etc. Dental pulp stem cells are a kind of mesenchymal stem cells. Autologous dental pulp stem cells (dental pulp stem cells, DPSCs) not only have the sa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0663C12N2500/80C12N2501/11C12N2501/115C12N2501/998
Inventor 曾皓宇沈振波刘园月林燕纯叶凌辛嫚刘素娜郭艳正
Owner GUANGDONG PROCAPZOOM BIOSCIENCES CO LTD
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