High-flux polypeptide synthesis device and use method thereof

Abstract

The invention belongs to the field of polypeptide synthesis, and particularly relates to a high-flux polypeptide synthesis device and a using method thereof. The device comprises ship-shaped reactors, fixing devices, a shaking device, a waste liquid collecting device and a connecting pipe, and the cross sectional area of openings in the two ends of the ship-shaped reactor is less than that of reactor cavities; and a plurality of reactors are placed in the shaking device capable of keeping constant temperature by utilizing fixing devices. A synthesis device is applied in a polypeptide synthesis process, so that reaction conditions can be stably controlled, raw materials and reagents are saved, the synthesis requirements of a large amount of or multiple types of polypeptides can be simultaneously met, the stability and efficient synthesis of the polypeptides are facilitated, and the method can be widely applied to the technical field of high-flux synthesis of polypeptides, especially difficult peptides.

Description

technical field [0001] The invention belongs to the field of polypeptide synthesis, and in particular relates to a high-throughput polypeptide synthesis device and its application method. Background technique [0002] In the early 1980s, the first true peptide synthesizer appeared in the world. From this, the solid-phase synthesis of peptides entered a rapid development, and after the second and third generations, many different types of peptide solid-phase synthesis devices evolved. [0003] In 2004, Hangzhou Zhongpei Company disclosed a high-throughput peptide synthesis method and device. This high-throughput peptide synthesis device mainly consists of a peptide reactor, a peptide chip, and a peptide eluter (Li Xiang et al., High-throughput peptide synthesis Method and device, CN1490327 A, 2004.04.21). In 2018, Nanjing Peptide Biotechnology Co., Ltd. disclosed a high-throughput solid-phase peptide synthesis device, which includes a constant temperature tube rack, peptide ...

AI Technical Summary

Problems solved by technology

In 2018, Runhui Biotechnology Co., Ltd. disclosed a continuous high-throughput peptide synthesis device, which solved the technical problems of complex operation, high cost, and low synthesis efficiency of existing peptides. It is equipped with reaction columns, vacuum pumps, waste A liquid collection device, a reagent storage device, and a waste liquid diversion pipe are also provided with a peristaltic pump. The reaction column is provided with an upper column, a lower column, a first sand core device and a second sand core device, and the lower column is a funnel-shaped structure ( Ji Shengli et al., A continuous high-throughput peptide synthesis device, CN 207619301 U, 2018.07.17)

[0004] Yet all there are some problems or defectives in these disclosed devices: 1. under electrostatic action, resin can agglomerate in reaction process, and solid-liquid can not mix evenly, then need to increase the pressure of nitrogen to eliminate electrostatic action; Yet, higher The high pressure will "blow" the polypeptide-solid phase carrier above the liquid surface of the reactor, resulting in sticky walls, and finally the sticky resin cannot participate in the reaction; 2. Long-term nitrogen bubbling, the solvent evaporates rapidly, and the solid-liquid mixing is uneven , but the synthesis fails; at the same time, a large amount of solvent volatilizes, the production environment is harsh, and it is not conducive to environmental protection; 3. The whole process continuously inputs nitrogen, which consumes a lot of nitrogen and increases the operating cost

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