Application of Wulan thirteen-ingredient decoction powder in preparation of medicine for protecting renal injury caused by primary hypertension

Abstract

The invention discloses application of Wulan thirteen-ingredient decoction powder in preparation of a medicine for protecting renal injury caused by primary hypertension. Results show that the Wulan thirteen-ingredient decoction powder not only can reduce the blood pressure of SHR, but also can reduce the blood urea nitrogen and creatinine level of SHR, alleviate renal injury and renal fibrosis caused by SHR hypertension, and play a role in protecting the kidney. As for in-vitro drug effect evaluation, hypertension injury modeling and drug administration are conducted on the HEK293 cells through Ang II on the basis of early-stage network pharmacology analysis and prediction, then an immunofluorescence experiment is carried out, and the effect of the Wulan thirteen-ingredient decoction powder on AHR protein expression in Ang II induced HEK293 cell hypertension injury is analyzed by utilizing a high-content imaging technology; and results show that AHR expression can be increased in virtue of a medium-dose (0.0415 mg / mL) Wulan thirteen-ingredient decoction powder administration group and a low-dose (0.0104 mg / mL) Wulan thirteen-ingredient decoction powder administration group.

Description

technical field [0001] The invention relates to the field of preparation of medicaments, in particular to the application of Wulan Shisanwei Decoction powder in the preparation of essential hypertension kidney damage protection medicine. Background technique [0002] Mongolian medicine Wulan thirteen flavor soup powder, composed of Tumuxiang, loquat leaves, flavescens, nasturtium, toosendan, comfrey antler, pearl stem, gardenia, myrobalan, acorn kernel, kaempferia, madder, comfrey Composed of thirteen medicinal materials, it has the effect of clearing blood and heat, and can be used for blood heat, headache, red eyes, and hypertension. Among them, gardenia and acorn kernels are used as monarch medicines, which have the effect of clearing blood; Tumuxiang, pearl stems, kaempferia, flavescens, toosendan, and nasturtium are used as minister medicines, which have the effect of promoting the maturity of fever; comfrey, Rubia, loquat leaves, and comfrey are used as adjuvant drugs...

AI Technical Summary

Problems solved by technology

According to literature reports, the current research on the effective substance basis of Wulan Shisanwei Decoction mainly focuses on the qualitative and quantitative analysis of a certain component, as well as the study of clinical drug effect observation, but the Mongolian medicine compound is taken as a whole, and the drug effect / Taking pharmacodynamics as an indicator, the in-depth research on the material basis and mechanism of action of Mongolian medicine compound Wulan Shisanwei Decoction powder for antihypertensive and target organ protection has not been reported yet.

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