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Polyaryl compound in purslane and extraction and separation method thereof

A polyaryl compound and separation method technology, which is applied in the directions of food extraction, ether separation/purification, food ingredients containing natural extracts, etc., can solve the problems of low structural novelty and the like, and achieves environmentally friendly process methods, simple and fast operation methods. Effect

Pending Publication Date: 2022-01-25
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the chemical components isolated from purslane are known, and the structural novelty is low. Therefore, the development and isolation of compounds in purslane are urgently needed

Method used

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  • Polyaryl compound in purslane and extraction and separation method thereof
  • Polyaryl compound in purslane and extraction and separation method thereof
  • Polyaryl compound in purslane and extraction and separation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The present invention provides polyarylate compound, molecular formula is C 60 h 58 O is named 1,1'-oxybis(2-((R)-1-phenylethyl)-4,5-bis((S)-1-phenylethyl)phenyl), and its chemical structure is:

[0036] .

[0037] The polyarylate is named 1,1'-oxybis(2-((R)-1-phenylethyl)-4,5-bis((S)-1-phenylethyl)phenyl) according to the structure, and Table 1 is the NMR data of polyarylates: 1 H-NMR with 13 C-NMR in DMSO.

[0038] Table 1: NMR data of the compound 1,1'-oxybis(2-((R)-1-phenylethyl)-4,5-bis((S)-1-phenylethyl)phenyl) of the present invention

[0039] .

[0040]Structural identification and deduction of the polyaryl compound 1,1'-oxybis(2-((R)-1-phenylethyl)-4,5-bis((S)-1-phenylethyl)phenyl) of the present invention.

[0041] 1,1'-oxybis(2-((R)-1-phenylethyl)-4,5-bis((S)-1-phenylethyl)phenyl): The compound is a white powder, easily soluble in methanol. UHPLC-ESI-Q-TOF-MS gave m / z: 795.4558[M-H] + (C 60 h 59 o + , the calculated value is 795.4561) of the q...

Embodiment 2

[0050] Example 2 Neuroprotective effect of polyaryl compounds of the present invention.

[0051] 1 main material.

[0052] 1.1 Drugs and reagents: The new alkaloid compounds used in the experiment were prepared by the above method, with a purity of 90-99%, weighed accurately, and diluted with DMSO to the required solutions for the following dosage groups. DMEM high-glucose medium, fetal bovine serum (Hyclone Company, USA); penicillin, streptomycin (Hangzhou Sijiqing Company), phosphate buffer saline (PBS), (Wuhan Boster Co., Ltd.), ROS detection kit (Haimen Bi Yuntian Reagent Company).

[0053] 1.2 Cell lines: Human neuroblastoma cell lines (SH-SY5Y, IMR-32) (Shanghai Cell, Chinese Academy of Sciences).

[0054] 1.3 Grouping: divided into control group, H 2 o 2 Damage model group and experimental group.

[0055] 2 Experimental methods.

[0056] 2.1 Cell culture, DMEM high-glucose medium, add 10% fetal bovine serum, 1% antibiotics (100U / mL penicillin and 100μg / mL streptom...

Embodiment 3

[0070] Example 3 Anticholinesterase effect of compounds of the present invention.

[0071] 1. Main materials.

[0072] 1.1. Drugs and reagents: The polyarylates used in the experiment were prepared by the above method with a purity of 90-99%. Sodium dihydrogen phosphate, disodium hydrogen phosphate (Sinopharm Chemical Reagent Co., Ltd.), physostigmine (Hanxiang Biotechnology ), phosphorus 5,5'-dithiobis(2-nitrobenzoic acid) (Dithiobisnitrobenzoic acid, DTNB, Shanghai Jinsui Biotechnology Co., Ltd.), acetylcholinesterase (AChE) and iodide thioacetylcholine (Acetylthiocholine iodide , ATCI, Dalian Meilun Biotechnology Co., Ltd.).

[0073] 1. Grouping: divided into blank group, control group and sample group.

[0074] 2. Experimental method.

[0075] 2.1 Sample preparation.

[0076] Precisely weigh the sample and 0.11 mg of physostigmine, respectively, and use methanol as a solvent to prepare five gradient concentrations of 2.5 μM, 5.0 μM, 10.0 μM, 20.0 μM and 40.0 μM. Accur...

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Abstract

The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to a novel polyaryl compound extracted, separated and identified from purslane and an extraction and separation method thereof. The molecular formula of the polyaryl compound is C60H58O, 1,1'-oxybis(2-((R)-1-phenylethyl)-4,5-bis ((S)-1-phenylethyl)phenyl). The invention further provides an extraction and separation method of the polyaryl compound. The polyaryl compound is prepared by sequentially adopting alcohol solvent decoction reflux extraction, macroporous resin column chromatography, silica gel column chromatography, ODS medium-pressure column and hydroxypropyl dextran column chromatography purification and liquid phase separation. The structure of the compound is determined by methods such as 1H-NMR, 13C-NMR, UHPLC-ESI-Q-TOF-MS and the like. The compound has potential neuro-protection activity, cholinesterase resistance activity and the like, a preparation method is provided, and a primer and a theoretical basis are provided for developing new drugs and new components.

Description

technical field [0001] The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to polyarylates extracted, separated and identified from purslane medicinal materials and an extraction and separation method thereof. Background technique [0002] purslane( Portulaca oleracea L.), also known as long-lived vegetable and horse amaranth, is a plant of the family Portulaca oleracea. Purslane likes fertile soil, is resistant to drought and waterlogging, has strong vitality, is widely distributed, and is rich in resources, and it is more common in Northeast my country. Purslane can be used as both medicine and food, and it is one of the wild plants with the same origin of medicine and food designated by the Ministry of Health of my country. The 2020 edition of "The Pharmacopoeia of the People's Republic of China" records that the dried aerial part of purslane is used as medicine. It has the effects of clearing away heat and de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C43/275C07C41/34C07C41/36A61P25/00A61P21/04A61P27/06A61P25/28A23L33/105
CPCC07C43/275C07C41/34C07C41/36A61P25/00A61P21/04A61P27/06A61P25/28A23L33/105A23V2002/00A23V2200/322A23V2200/30A23V2250/21A23V2300/14A23V2300/50
Inventor 张文洁崔馨予郭胜男兰秀娟英锡相
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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