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Cell preserving fluid capable of maintaining cell viability as well as preparation method and application of cell preserving fluid

A technology of cell activity and preservation solution, which is applied in the field of cell preservation solution for maintaining cell activity and its preparation, which can solve the problems of cell membrane damage, improvement of preservation solution effect, and ice crystal generation, so as to maintain cell activity, enhance cell protection, Preserve the best results

Active Publication Date: 2022-01-14
GUANGDONG UNISUN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under low temperature conditions, the solution inside and outside the cell is prone to ice crystals, which can damage the cell membrane.
Therefore, it is necessary to store the cells in a suitable preservation solution. Although there are various preservation solutions on the market, the effect of these preservation solutions needs to be improved.

Method used

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  • Cell preserving fluid capable of maintaining cell viability as well as preparation method and application of cell preserving fluid
  • Cell preserving fluid capable of maintaining cell viability as well as preparation method and application of cell preserving fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The present embodiment provides a cell preservation solution for maintaining cell viability, which includes components of the following concentrations: 20 mmol / L of 4-hydroxyethylpiperazineethanesulfonic acid, 3 mmol / L of magnesium chloride, and 90 mmol of propylene glycol alginate / L, raffinose 15 mmol / L, adenosine triphosphate disodium 3 mmol / L, reduced glutathione 2 mmol / L, α-lipoic acid 0.1 mmol / L, hydroxyethyl starch phosphate 40 g / L and schisandra B 10μmol / L.

[0037] Its preparation method includes:

[0038] Weigh 4-hydroxyethylpiperazineethanesulfonic acid, magnesium chloride, propylene glycol alginate, raffinose, adenosine triphosphate disodium, reduced glutathione, α-lipoic acid, and hydroxyethyl phosphate starch respectively according to the specified concentration. Dissolve in ultra-pure water; adjust the pH to 7.2-7.4, add Schizandrin B, sterilize and seal it for storage; prepare a cell preservation solution with an osmotic pressure of 305-315mmol / L.

Embodiment 2-4

[0040] The cell preservation solution for maintaining cell activity provided in Examples 2-4 has basically the same components as in Example 1, except that the extracts of traditional Chinese medicines are different.

[0041] In Example 2, the Chinese medicine extract is quercetin 10 μmol / L.

[0042] In Example 3, the Chinese medicine extract is resveratrol 10 μmol / L.

[0043] In Example 4, the Chinese medicine extracts are resveratrol 5 μmol / L and quercetin 5 μmol / L.

Embodiment 5

[0045] The present embodiment provides a cell preservation solution for maintaining cell viability, which includes components of the following concentrations: 15 mmol / L of 4-hydroxyethylpiperazineethanesulfonic acid, 2 mmol / L of magnesium chloride, and 85 mmol of propylene glycol alginate / L, raffinose 12 mmol / L, adenosine triphosphate disodium 2 mmol / L, reduced glutathione 1 mmol / L, α-lipoic acid 0.1 mmol / L, hydroxyethyl starch phosphate 33 g / L and Schisandra chinensis B 5μmol / L.

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PUM

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Abstract

The invention discloses a cell preserving fluid for keeping cell activity and a preparation method and application of the cell preserving fluid, and relates to the technical field of cell preservation and transportation. The cell preserving fluid is prepared from the following components: 10 to 30 mmol / L of 4-(2-hydroxyethyl)piperazine ethanesulfonic acid, 2 to 5 mmol / L of magnesium salt, 80 to 100 mmol / L of propylene glycol alginate, 10 to 20 mmol / L of melitose, 2 to 5 mmol / L of disodium adenosine triphosphate, 1 to 3 mmol / L of reduced glutathione, 0.1 to 0.2 mmol / L of alpha-lipoic acid, 30 to 50 g / L of hydroxyethyl phosphate starch and 5 to 20 [mu]mol / L of traditional Chinese medicine extract. The cell preserving fluid provided by the invention can effectively maintain cell activity at 4 DEG C, and the components of the cell preserving fluid are safe and non-toxic to human bodies, so subsequent use of cells is facilitated; and the cell preserving fluid is suitable for preservation or transportation of cells and has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of cell preservation and transportation, in particular to a cell preservation solution for maintaining cell activity and its preparation method and application. Background technique [0002] Cell culture and preservation techniques are widely used in various fields of life science research, such as vaccine development, artificial organs and regenerative medicine. Cryopreservation is currently a common method for long-term cell preservation, which can reduce genetic variation and morphological changes caused by subculture of cells, and avoid senescence or malignant transformation of limited cell lines. However, the cryopreservation and recovery process of cells will cause great damage to the cells, reduce the lipid content in the cell membrane, produce peroxidation, and affect the structure and metabolic pathways of the cells at the same time. Low temperature preservation reduces the risk of cell damage and ...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0221A01N1/0226
Inventor 彭青高毅张莹李阳周树勤
Owner GUANGDONG UNISUN BIOTECHNOLOGY CO LTD
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