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Preparation method of photoelectrochemical sensor for detecting 17 beta-estradiol by using protoporphyrin modified bismuth doped strontium titanate

A photoelectrochemical and strontium titanate technology, applied in the field of photoelectrochemical sensor preparation

Inactive Publication Date: 2022-01-11
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of protoporphyrin-modified bismuth-doped strontium titanate materials in photoelectrochemical sensors has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Preparation of photo chemical sensor embodiment

[0043] (1) a bismuth-doped bulk cubic strontium titanate prepared nanomaterials

[0044] Take 0.7 g strontium hydroxide, 0.1 g of titanium dioxide (P25), 0.01 g of bismuth nitrate was dissolved in 40 mL ultrapure water was stirred at room temperature for 5 min, to obtain a solution A; take 1.0 g of sodium hydroxide dissolved in 40 mL of ultrapure water , yielding a solution B; solution A and B two uniformly mixed into the autoclave, the reaction for 16 h at 160 ° C, after completion of the reaction, allowed to cool, the product was washed with ethanol, and ultrapure water samples over anhydrous until neutral, dried in vacuo 10 h at 50 ° C, to prepare the bismuth-doped bulk cubic strontium titanate nanomaterials;

[0045] (2) Preparation of strontium titanate material protoporphyrin modified bismuth doped

[0046] Preparation of 1 mg taken bismuth strontium titanate doped with nano-material was dissolved in 1 mL ult...

Embodiment 2

[0060] Example 2 Preparation of photo chemical sensor embodiment

[0061] (1) a bismuth-doped bulk cubic strontium titanate prepared nanomaterials

[0062] Take 0.8 g strontium hydroxide, 0.2 g of titanium dioxide (P25), 0.1 g of bismuth nitrate was dissolved in 40 mL of ultrapure water, 10 min of stirring at room temperature, to obtain a solution A; take 2.0 g of sodium hydroxide dissolved in 40 mL of ultrapure water , yielding a solution B; solution A and B two uniformly mixed into the autoclave, the reaction for 18 h at 180 ° C, after completion of the reaction, allowed to cool, the product was washed with ethanol and ultrapure water samples until neutral, dried in vacuo 12 h at 80 ° C, to prepare the bismuth-doped bulk cubic strontium titanate nanomaterials;

[0063] (2) Preparation of strontium titanate material protoporphyrin modified bismuth doped

[0064] Preparation take 3 mg of bismuth strontium titanate doped with nano-material was dissolved in 3 mL ultrapure water, the...

Embodiment 3

[0078] Example 3 Preparation of photo chemical sensor embodiment

[0079] (1) a bismuth-doped bulk cubic strontium titanate prepared nanomaterials

[0080] Take 1.0 g strontium hydroxide, 0.3 g of titanium dioxide (P25), 0.2 g of bismuth nitrate was dissolved in 40 mL ultrapure water was stirred at room temperature for 15 min, to obtain a solution A; take 5.0 g of sodium hydroxide dissolved in 40 mL of ultrapure water , yielding a solution B; solution A and B two uniformly mixed into the autoclave, the reaction for 24 h at 220 ° C, after completion of the reaction, allowed to cool, the product was washed with ethanol and ultrapure water samples until neutral, and dried 14 h in vacuo at 100 ° C, to prepare the bismuth-doped bulk cubic strontium titanate nanomaterials;

[0081] (2) Preparation of strontium titanate material protoporphyrin modified bismuth doped

[0082]Take 5 mg prepared hydrazine nanomaterials in 5 ml of ultra-pure water, and then add 5 ml of 5 ml of N, N-dimethylf...

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Abstract

The invention relates to a preparation method of a photoelectrochemical sensor for detecting 17 beta-estradiol by using protoporphyrin modified bismuth doped strontium titanate. The protoporphyrin modified bismuth doped strontium titanate nano material is used as a substrate material to obtain light current, and the photoelectric conversion efficiency of the cubic bismuth tungstate sensitized by protoporphyrin and doped with bismuth ions is greatly improved. Besides, barium chromate is used as a marker to mark a 17beta-estradiol second antibody, wherein the antibody, the first antibody and an antigen are jointly modified in a 96 microwell plate, so that when diluted hydrochloric acid is used for treating the 96 microwell plate, Cr (VI) is released and interacts with quercetin in a test base solution, polarity conversion of detection light current signals is achieved, and sensitive detection of 17beta-estradiol is achieved, the detection limit being 3.5 fg / mL.

Description

Technical field [0001] The present invention relates to a method for preparing a photochemical sensor of a protoporphyrin-modified bismuth-blending titanate detection 17β-estradiol. Specifically, it is used as a base photosensitive material using protoporphoporphyrin-modified tantanate nanomaterial as a base photosensitive material, and the second antibody is labeled with chromate as a label, and the antibody is commonly modified in the 96 microplate of the first antibody and antigen. When treated with an acid, the chromate is dissolved and the Cr (VI) is dissolved, and Cr (VI) is added to the solution in the solution to interact with the quercetin, so that the current polarity changes, and prepared a detection female two. The signal polar transformable photoelectrochemical sensor of the alcohol belongs to the field of new functional materials and biosensor detection. Background technique [0002] The 17β-estradiol (E2) has strong estrogen-active endogenous estrogen, which has po...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/32G01N33/74
CPCG01N27/30G01N27/32G01N33/743
Inventor 魏琴徐芮马洪敏吴丹任祥
Owner UNIV OF JINAN
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