Method for detecting Cr 6+ by using nitrogen-doped carbon quantum dot fluorescent probe

Abstract

The embodiment of the invention discloses a method for detecting Cr 6+ by using a high-quantum-yield nitrogen-doped carbon quantum dot fluorescent probe, which is characterized by comprising the following steps: preparing a high-quantum-yield nitrogen-doped carbon quantum dot fluorescent probe detection reagent; mixing a to-be-detected sample and the Hg 2+ masking agent, adding the mixture into the detection reagent, mixing, and measuring the fluorescence intensity change of the mixed solution by using a fluorescence intensity detector; when the fluorescence intensity of the mixed solution is weakened, determining that the sample to be detected contains Cr 6+, or when the fluorescence intensity of the mixed solution does not change, determining that the sample to be detected does not contain Cr 6+. According to the present invention, the qualitative or quantitative detection of Cr 6+ is realized through naked eye observation or by virtue of a fluorospectro photometer, so that the method has the advantages of high specificity, high sensitivity, rapidness, simplicity in operation, low cost and the like.

Description

technical field [0001] The embodiment of the present invention relates to the field of chemical technology, especially a nitrogen-doped carbon quantum dot fluorescent probe for detecting Cr 6+ Methods. Background technique [0002] With the rapid development of modern industry, heavy metal pollution is becoming more and more serious. Among them, chromium is an industrial raw material widely used in metallurgy, electroplating, tanning, pigment and dye production, plastic synthesis, and ceramic preparation, and is a common heavy metal pollutant. Normally, chromium mainly exists in two valence states (Cr 3+ 、Cr 6+ ) exist in nature. where Cr 3+ Although it is an essential trace element for the human body, it can regulate the insulin content in blood sugar and promote the metabolism of lipid and protein, but excessive intake of Cr 3+ It can cause oxidative damage to cellular components (such as lipids, proteins, and DNA) and pose a hazard to human health. In addition, stu...

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Problems solved by technology

Although these methods can accurately determine Cr 6+ However, it has the disadvantages of expensive detection equipment, complex detection process, and the need for professional personnel to operate, which still has certain limitations for home diagnosis or on-site scale detection.

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