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Preparation of boron-nitrogen-doped graphene quantum dot and application of boron-nitrogen-doped graphene quantum dot in drugs for boron neutron capture therapy

A technology of graphene quantum dots and therapeutic drugs, applied in the field of chemical and biomedical tumor diagnosis and treatment, to achieve good low toxicity, good biocompatibility, and realize the effect of diagnosis

Active Publication Date: 2021-12-28
INST OF HIGH ENERGY PHYSICS CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently no boron delivery agent that can simultaneously meet the above requirements

Method used

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  • Preparation of boron-nitrogen-doped graphene quantum dot and application of boron-nitrogen-doped graphene quantum dot in drugs for boron neutron capture therapy
  • Preparation of boron-nitrogen-doped graphene quantum dot and application of boron-nitrogen-doped graphene quantum dot in drugs for boron neutron capture therapy
  • Preparation of boron-nitrogen-doped graphene quantum dot and application of boron-nitrogen-doped graphene quantum dot in drugs for boron neutron capture therapy

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]The boron-nitrogen-doped graphene quantum dots proposed by the present invention are synthesized by vapor-phase precipitation, and the preparation method specifically includes the following steps:

[0040] a. Dissolve 0.25g of soluble starch in 25ml of water, mix and stir to obtain a solution, add the solution to a reaction kettle at a temperature of 190°C for a hydrothermal reaction for 120min to obtain a reaction solution, centrifuge and filter the reaction solution, and remove the reaction solution After the lower layer is solid in the liquid, the upper layer solution is freeze-dried to obtain graphene quantum dot powder;

[0041] b. Mix 0.1g graphene quantum dot powder with 100ml 10 B boric acid aqueous solution (concentration is 1mg / mL, 10 The abundance of B is 95%) after uniform mixing, freeze-drying to obtain a powdery product, the powdery product is placed in a tube furnace, the temperature of the tube furnace is set to be 900 ° C and NH is introduced into the f...

Embodiment 2

[0047] In order to verify the application effect of the boron-nitrogen-doped graphene quantum dots prepared in Example 1 in the BNCT treatment process, human breast cancer cells 4T1 were selected to be cultured with BNGOs. The results showed that BNGOs can target breast cancer cells and perform fluorescence. imaging.

[0048] The effect of BNGO on the survival rate of breast cancer cells 4T1 image 3 As shown, the IC50 of breast cancer cell 4T1 after BNCT treatment was 176.45ug / mL.

[0049] During the experiment, the cell morphology of breast cancer cells containing boron-nitrogen-doped graphene quantum dots and the cell morphology of breast cancer cells without boron-nitrogen-doped graphene quantum dots were observed after a single BNCT treatment for 24 hours. Figure 4 Shown is the cell morphology of breast cancer cells without BN-doped graphene quantum dots after neutron irradiation. It can be seen that breast cancer cells that have not been incubated with BNGO drugs are s...

Embodiment 3

[0051] In order to study the boron-nitrogen-doped graphene quantum dots prepared in Example 1 the influence of breast cancer cell 4T1 monoclonal. A control experiment was set up. In the experimental group of the control experiment, the cultured breast cancer cells 4T1 and BNGOs were cultured, incubated for 24 hours, then resuspended in the culture medium, irradiated by the neutron flow of the accelerator for 1 hour, and then retransplanted into the culture medium. Culture in medium, record the number of breast cancer cells 4T1 at this time with a cell counting plate, culture the irradiated breast cancer cells 4T1 in the culture medium for 7 days, and use crystal violet staining method to stain the breast cancer cells 4T1 in the culture medium At this time, the number of breast cancer cells 4T1 in the medium was counted. Since crystal violet can stain the cytoplasm of the cells, the number of cells can be determined by observing the stained area of ​​the medium. In order to bet...

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Abstract

The invention discloses preparation of a boron-nitrogen-doped graphene quantum dot and application of the boron-nitrogen-doped graphene quantum dot in drugs for boron neutron capture therapy, and relates to the field of chemical and biomedical tumor diagnosis and treatment. According to the invention, a graphene quantum dot and a 10B boric acid aqueous solution are used as raw materials to synthesize the boron-nitrogen-doped graphene quantum dot; the boron-nitrogen-doped graphene quantum dot is high in boron content, has excellent optical properties, good biocompatibility and low toxicity, realizes in-vivo fluorescence imaging and can target to breast cancer tumors and be enriched at breast cancer tumor positions, but however, the boron-nitrogen-doped graphene quantum dot in healthy tissue can be rapidly metabolized and discharged, so the boron-nitrogen-doped graphene quantum dot can be used as a novel boron delivery agent suitable for BNCT treatment, and diagnosis of breast cancer tumors and BNCT treatment are realized.

Description

technical field [0001] The invention relates to the field of chemical and biomedical tumor diagnosis and treatment, in particular to the preparation of boron-nitrogen-doped graphene quantum dots and their application in boron neutron capture therapeutic drugs. Background technique [0002] Boron neutron capture therapy (BNCT), as a new type of precise tumor treatment method, 10 B captures thermal neutrons and undergoes nuclear fission reactions to produce alpha particles and 7 Li recoils from the nucleus, thereby selectively killing tumor cells. BNCT is based on nuclear capture and nuclear fission reactions, due to non-radioactive 10 B atom (20% in natural boron element) can undergo neutron capture and then nuclear fission, 10 B is activated after being irradiated by neutrons of a certain energy 11 B and then fission into high-energy alpha particles and 7 Li recoils the nucleus, and the nuclear reaction formula is: [0003] B 10 +n th →[B 11 ] * →α+Li 7 +2.31MeV ...

Claims

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Application Information

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IPC IPC(8): C09K11/65B82Y20/00B82Y40/00C01B32/15A61K41/00A61P35/00
CPCC09K11/65B82Y20/00B82Y40/00C01B32/15A61K41/0095A61P35/00
Inventor 邢更妹李嘉程李娟崔荣丽陈奎常亚男
Owner INST OF HIGH ENERGY PHYSICS CHINESE ACADEMY OF SCI
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