Application of citral or citral-containing plant extract in preparation of plant resistance inducer

A plant extract, plant resistance induction technology, applied in the directions of plant growth regulators, plant growth regulators, botanical equipment and methods, etc., can solve problems such as further development

Active Publication Date: 2021-12-28
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of agricultural use, it has been reported in the literature that citral has a certain antibacterial effect on plant pathogenic fungi, etc., but there are no relevant research reports on its activity of inducing plant disease resistance, inducing plant stress resistance and promoting plant growth. It is used in agriculture, horticulture and Applications in the forestry field need to be further developed

Method used

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  • Application of citral or citral-containing plant extract in preparation of plant resistance inducer
  • Application of citral or citral-containing plant extract in preparation of plant resistance inducer
  • Application of citral or citral-containing plant extract in preparation of plant resistance inducer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1: Anti-TMV activity determination of citral and plant extract containing citral

[0044] (1) Protective activity

[0045] Prepare 0.02mg / mL, 0.1mg / mL and 0.5mg / mL citral and citral-containing essential oil and extract solutions, select healthy 5-6-leaf stage tobacco leaves with consistent growth, and inoculate and dilute them 48 hours after spraying the agent 2000-fold TMV solution, the blank control was treated with water, and the positive control was 0.1 mg / mL chitosan oligosaccharide. Each treatment was inoculated with 3 leaves and repeated 3 times. After 3 days, the number of dead spots was counted to calculate the inhibition rate.

[0046] (2) in vitro passivation activity

[0047] Prepare 0.02mg / mL, 0.1mg / mL and 0.5mg / mL citral and citral-containing essential oil and extract solutions, and mix with equal volumes of 1000-fold diluted TMV solution. After standing at room temperature for 1 hour, inoculate the same-growing, healthy 5-6-leaf stage tobacc...

Embodiment 2

[0056] It can be seen from Table 1 that citral has a good protective effect on TMV, and the disease prevention effect is as high as 76.27%, indicating that early application can make tobacco have a certain disease resistance effect. However, the passivation effect of citral on TMV was average, which indicated that citral did not cause direct damage to TMV mitochondria or the damage effect was weak, which was far less than the disease resistance effect caused by early application. Plant extracts containing citral also have certain antiviral activity, among which Litsea cubeba essential oil and extracts are the most prominent Example 2: Determination of anti-TMV activity of tobacco induced by citral and citral-containing essential oils and extracts

[0057] The heart leaf tobacco was selected for the verification of the induced disease resistance activity. The heart leaf tobacco can form virus scabs, and the symptom on the common tobacco is mosaic. Different symptoms use differen...

Embodiment 3

[0066] Embodiment 3: Citral causes the activity change of tobacco defense enzyme POD

[0067] After spraying 0.02mg / mL, 0.1mg / mL and 0.5mg / mL citral to the leaves, the leaves were taken on the 1st, 3rd, 5th, 7th, 9th, and 11th day respectively, and the activity changes of the defense enzymes PAL and POD were detected. Weigh 5 g of plant leaves, cut them into pieces and place them in a frozen mortar, add a small amount of quartz sand, and add a total of 5 mL of 0.1 mol / L, pH 5.5 acetic acid-sodium acetate buffer in 2 to 3 times. After being ground into a homogenous slurry, centrifuge at 12,000 r / min for 15 minutes at 4°C, and the supernatant is the crude enzyme extract. Take the test tube and add 3mL 25mmol / L guaiacol and 5mL enzyme extract, then add 200μL, 5mol / L H 2 o 2 The solution mixes rapidly to initiate the reaction. Using distilled water as a reference, start recording the value of the reaction system at a wavelength of 470nm at 15s of reaction time, record once ever...

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Abstract

The invention discloses application of citral and/or a citral-containing plant extract in preparation of a plant resistance inducer. The application range of the plant resistance inducer comprises induction of plant disease resistance, induction of plant stress resistance or promotion of plant growth. The plant disease resistance inducing activity, plant stress resistance inducing activity and plant growth promoting activity of the citral and the citral-containing plant essential oil or extract are determined by biological activity determination, physiological and biochemical testing and other methods; a pesticide preparation, an auxiliary agent and a biostimulant which take citral and citral-containing plant essential oil or extract as active ingredients are prepared, and are applied in the field.

Description

technical field [0001] The invention belongs to the field of plants and applications thereof, in particular to the application of citral or plant extracts containing citral to prepare plant inducers. Background technique [0002] Plant essential oils exist widely in nature, and most plant families contain plant essential oils, for example, plant families such as Magnoliaceae, Gramineae, and Piperaceae are rich in essential oils. Plant essential oils are complex mixtures, usually composed of many kinds of organic compounds, including large polar, small polar and non-polar substances. Terpenes composed of monoterpenes and sesquiterpenes are more common in essential oils, but the specific components also vary with different essential oils. It is the main component of various plant essential oils; aromatic compounds (aldehydes, alcohols, methoxy derivatives, etc.), the content is second only to terpene compounds, such as about 80% of cinnamaldehyde in cinnamon essential oil, li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N35/02A01N61/00A01P21/00A01N47/18A01N47/14A01N47/26A01N63/50A01P3/00A01G7/06
CPCA01N35/02A01N61/00A01N47/18A01N47/14A01N47/26A01N63/50A01G7/06Y02A50/30
Inventor 闫合姜悦何姗姬晓兰罗伟刘斌马志卿冯俊涛
Owner NORTHWEST A & F UNIV
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