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Cell transfection device, cell transfection method and micro-channel manufacturing method

A micro-channel and cell technology, applied in chemical instruments and methods, biochemical cleaning devices, biochemical equipment and methods, etc., can solve problems such as low transfection efficiency of nano-exogenous substances

Active Publication Date: 2021-12-21
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Based on this, the object of the present invention is to provide a cell transfection device and method, which is used to solve the problem that the traditional fixed-width microchannel in the prior art is only suitable for The problem of low transfection efficiency of macromolecular nano-exogenous substances for cells of a specific size or deformability

Method used

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  • Cell transfection device, cell transfection method and micro-channel manufacturing method
  • Cell transfection device, cell transfection method and micro-channel manufacturing method
  • Cell transfection device, cell transfection method and micro-channel manufacturing method

Examples

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Embodiment 1

[0047] see figure 2 , shows the cell transfection device in the first embodiment of the present invention, the device includes a microfluidic chip 200, and a tunable squeezer that is located above the microfluidic chip 200 and squeezes the microfluidic chip 200 at a certain frequency Mechanism, the microfluidic chip 200 is provided with a microchannel, and a restricted space is provided in the microchannel, the cross-sectional size of the restricted space is not larger than the diameter of the cell, and a plurality of foreign substances are arranged in the microchannel, specifically, The foreign substance is a nanomolecule to be transfected. The confined space is located in the middle of the microchannel, and the size of the cross section is smaller than that of the microchannel under normal conditions. When the cells flow through the confined space, the confined space Limiting cells, the adjustable extrusion mechanism squeezes the microfluidic chip 200 and then squeezes the ...

Embodiment 2

[0056] Please check image 3 , shows the cell transfection method in the second embodiment of the present invention, the cell transfection method adopts the cell transfection device in the above embodiment, and the method includes steps S201 to S204:

[0057] S201. When the computer device acquires the waveform signal, convert the waveform signal into a control signal through the signal generator, and transmit the control signal to the piezoelectric driver;

[0058] S202. When the piezoelectric actuator receives the control signal, it converts the control signal into a fixed frequency and outputs it to the cantilever beam to drive the cantilever beam to move regularly, thereby driving the microneedle tip set at the end of the cantilever beam to regularly tap the microflow control chip;

[0059] S203. When the microneedle tip hits the microfluidic chip, the microfluidic channel in the microfluidic chip is squeezed, deforming the side wall of the microfluidic channel and squeez...

Embodiment 3

[0067] Please check Figure 5 , shows the method for manufacturing the microchannel in the third embodiment of the present invention, which is applied to the microchannel in the cell transfection device in the above embodiment, and the manufacturing method includes steps S301 to S304:

[0068] S301. Obtain the target photoresist, spin-coat the target photoresist to a certain thickness on the silicon wafer, and perform pre-baking treatment on the silicon wafer coated with the photoresist;

[0069] As a specific example, the polymer material PDMS is used to fabricate and process microchannels through MEMS soft lithography technology, such as Image 6 A schematic diagram of the processing of the microfluidic chip is shown. The MEMS processing method can mass-produce microfluidic chips, reducing the processing cost of microfluidic chips.

[0070] The thickness of the photoresist can be controlled by the type of SU-8 photoresist and the number of rotations of the spin coating, th...

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Abstract

The invention provides a cell transfection device, a cell transfection method and a micro-channel manufacturing method. The cell transfection device comprises a micro-fluidic chip and a tunable extrusion mechanism, a micro-channel is arranged in the micro-fluidic chip, a limited space is arranged in the micro-channel, and a plurality of allogenic materials are arranged in the micro-channel; the tunable extrusion mechanism comprises a micro needle tip, a piezoelectric actuator and a cantilever beam; and when the piezoelectric actuator vibrates, the micro needle tip is driven to move up and down, extrudes or is separated from the micro-fluidic chip, cell membranes of cells in the limited space are driven to be damaged to generate through holes, and the allogenic materials enter the cells through the through holes. According to the cell transfection device, the cell transfection method and the micro-channel manufacturing method, the micro-fluidic chip is regularly extruded or separated through the tunable extrusion mechanism, so that the width of the micro-channel is variable, the micro-channel is suitable for cells with different sizes, and the problem that a traditional micro-channel with a fixed width is only suitable for cells with specific sizes or deformation force and has low transfection efficiency on macromolecular nano allogenic materials is solved.

Description

technical field [0001] The invention relates to the technical field of biological transfection, in particular to a cell transfection device, a cell transfection method and a method for making a microfluidic channel. Background technique [0002] Microfluidic technology means that researchers can achieve physical loading such as mechanics and electricity on individual cells through exquisite structural design and advanced microelectronics technology. Microscale electrode technology, shear force loading and local heating technology combined with microfluidic technology can be used to create temporary perforation of individual cell membranes. [0003] Intracellular delivery is the process of transfecting nanoscale exogenous substances such as genes, proteins and biomacromolecules into the intracellular body of target cells and successfully expressing them. Cell transfection refers to the technology of introducing exogenous molecules such as DNA and RNA into eukaryotic cells. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36C12M1/00B01L3/00
CPCC12M23/16C12M41/48C12M35/04C12M23/20B01L3/5027B01L3/502707B01L2300/12
Inventor 陈建锋段仰康陈笑笑许文虎钟敏易美荣李小兵
Owner NANCHANG UNIV
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