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Conotoxin-entrapped chitosan nanoparticle emulsion and preparation method thereof

A technology of chitosan nanoparticles and conotoxin, which is applied in skin care preparations, medical preparations containing active ingredients, and pharmaceutical formulas, can solve problems such as unfavorable consumers' self-use, and promote practical application and promotion , enhanced percutaneous penetration, significant economic value

Pending Publication Date: 2021-12-17
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical method mainly realizes the transdermal penetration of active substances through particle introduction, ultrasonic introduction, etc., and the chemical method mainly uses absorption enhancers to increase the percutaneous penetration of active substances. However, physical methods often require special introduction equipment, which is not conducive to consumers’ daily life. self use

Method used

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  • Conotoxin-entrapped chitosan nanoparticle emulsion and preparation method thereof
  • Conotoxin-entrapped chitosan nanoparticle emulsion and preparation method thereof
  • Conotoxin-entrapped chitosan nanoparticle emulsion and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Take 50mg of conotoxin and 100mg of chitosan dissolved in 50ml of 1% acetic acid solution, slowly drop into 50ml of 0.1mg / ml sodium tripolyphosphate solution under magnetic stirring, adjust the pH to 5.5 after the addition, continue stirring for a while to get taro Spirotoxin chitosan nanoparticle suspension, add Tween 80 2g, hyaluronic acid 2g, glycerin 2g, sodium carboxymethyl cellulose 0.5g to dissolve, mix well, get water phase, keep warm at 70°C for later use; Put 2g of fatty acid, 2g of glycerol monostearate, 4.5g of isopropyl myristate, and 0.5g of vitamin E in a beaker, heat it in a water bath to 70°C to melt, and obtain an oil phase; Slowly add the water phase, stir while adding, and allow to cool to room temperature to obtain the chitosan nanoparticle emulsion loaded with conotoxin.

Embodiment 2

[0032] Take 50mg of conotoxin and 200mg of chitosan dissolved in 50ml of 1% acetic acid solution, slowly drop into 50ml of 0.5mg / ml sodium tripolyphosphate solution under magnetic stirring, adjust the pH to 5.5 after the addition, continue stirring for a while to get taro Spirotoxin chitosan nanoparticle suspension. Add 8g of polyethylene glycol 15-hydroxystearate, 2g of polyethylene glycol 1000, 6g of glycerin, and 1.5g of methylcellulose to dissolve, mix well to obtain the water phase, and keep it warm at 70°C for later use; take another 4g of soybean lecithin, Put 6g of glyceryl stearate, 19g of isopropyl palmitate, and 1g of liquid paraffin in a beaker, heat it in a water bath to 70°C to melt, and obtain an oil phase; slowly add the prepared oil phase to the water phase, adding While stirring, let it cool to room temperature to obtain the chitosan nanoparticle emulsion loaded with conotoxin.

Embodiment 3

[0034] Take 50mg of conotoxin and 150mg of chitosan dissolved in 50ml of 1% acetic acid solution, slowly drop into 50ml of 0.3mg / ml sodium tripolyphosphate solution under magnetic stirring, adjust the pH to 5.5 after the addition, continue stirring for a while to get taro Spirotoxin chitosan nanoparticle suspension. Add 8g of polyethylene glycol 15-hydroxystearate, 1g of carbomer 940, 4g of glycerin, and 1.0g of methylcellulose to dissolve, mix well to obtain the water phase, and keep it warm at 70°C for later use; take another 2g of soybean lecithin, stearin Put 1g of acid, 5g of propylene glycol, 10g of isopropyl palmitate, and 5g of isopropyl myristate in a beaker, heat it in a water bath to 70°C to melt, and obtain an oil phase; slowly add the prepared oil phase to the water phase , stir while adding, and let cool to room temperature to obtain the chitosan nanoparticle emulsion loaded with conotoxin.

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Abstract

The invention discloses a conotoxin-entrapped chitosan nanoparticle emulsion and a preparation method thereof, and belongs to the technical field of high polymer materials. The emulsion is prepared from the following raw materials in parts by weight: 1 part of conotoxin, 2 to 4 parts of chitosan, 0.1 to 0.5 part of sodium tripolyphosphate, 100 to 400 parts of greasy components, 40 to 160 parts of emulsifier, 80 to 200 parts of auxiliary emulsifier, 80 to 160 parts of humectant and 10 to 30 parts of thickening agent. According to the invention, the conotoxin is coated with the chitosan to prepare nano-particles, and the nano-particles are prepared into an emulsion, which promotes the conotoxin to continuously permeate into the deep layer of skin to play a role in removing wrinkles; meanwhile, the ingredients such as the auxiliary emulsifier and the humectant are added, which helps hydration of skin cuticle, so that transdermal permeation of the conotoxin nanoparticles is enhanced; all the raw materials act together and cooperate with one another, to promote the transdermal permeability of the conotoxin together, so that further practical application and popularization of the conotoxin are promoted, and the economic value is remarkable.

Description

technical field [0001] The invention belongs to the technical field of polymer materials, and in particular relates to a chitosan nanoparticle emulsion carrying conotoxin and a preparation method thereof. Background technique [0002] Aging is a natural phenomenon. With the increase of age, human skin will gradually age. The most obvious manifestation is the increase and deepening of facial wrinkles. Stay young. Removing facial wrinkles is undoubtedly the most direct and effective way to maintain a youthful appearance, so wrinkle removal products have attracted great attention from beauty and skin care professionals. [0003] In anti-wrinkle products, peptide products occupy an important position. Conotoxin is composed of 12-40 amino acids and rich in disulfide bonds. It is secreted by the venom glands of the cone snail venom tube and the inner wall of the poison sac. It can act on the muscle sodium ion valve channel Nav 1.4 and block neuromuscular stimulation. The curren...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/86A61K8/06A61K8/31A61K8/34A61K8/37A61K8/64A61K8/67A61K8/73A61K8/81A61Q19/08A61Q19/00
CPCA61K8/64A61K8/736A61K8/31A61K8/37A61K8/678A61K8/735A61K8/8147A61K8/86A61K8/345A61K8/731A61K8/068A61Q19/08A61Q19/00A61K2800/413
Inventor 姚继鹏张淳张增涛李海刚
Owner LINYI UNIVERSITY
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