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Frequency domain quantum weak measurement biomolecule sensor and measurement method thereof

A biomolecule and sensor technology, applied in the field of frequency domain quantum weak measurement biomolecule sensor and its measurement, can solve the problems of difficulty in restoring the optical path state, difficulty in integrating weak measurement methods, etc., and achieves improved sensitivity, improved robustness, The effect of improving stability

Active Publication Date: 2021-12-07
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

However, the weak measurement method itself has the problem that it is difficult to integrate. The main reason is that although the weak measurement method in the frequency domain has excellent measurement results, it needs to separate the spectrum during measurement. It is very sensitive to the spatial angle response of light, and it is easy to replace different samples. It affects the weak value state of the light path, and it is difficult to restore the state of the light path

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  • Frequency domain quantum weak measurement biomolecule sensor and measurement method thereof
  • Frequency domain quantum weak measurement biomolecule sensor and measurement method thereof
  • Frequency domain quantum weak measurement biomolecule sensor and measurement method thereof

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Experimental program
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Embodiment 1

[0094] The biomolecular sensor based on differential frequency domain quantum weak measurement provided by this embodiment has a structure such as figure 1 As shown, the sensor includes a light emitting device 1, a polarization state preparation device 2, a phase compensation system 3, a prism and flow channel device 4, a flow channel coupling element 5, a prism replacement device 6, a polarization state selection device 7, and a spectral frequency division device 8 and photodetection element 10. Wherein the light source generator 1 is a semiconductor laser, the polarization state preparation device 2 and the polarization state selection device 7 are both polarization attenuation plates, the phase compensation system 3 is a Soley-Babignet phase compensator, and the prism 4 is a positive triangular prism. The liquid is passed through and contacts with the reflective surface of the prism, the spectral frequency division device 8 is a dichroic mirror, and the photodetection eleme...

Embodiment 2

[0097] This embodiment is based on the quantum weak measurement technology, and the surface plasmon sensor based on the quantum weak measurement provided in Example 1 is used to measure the standard NaCl solution sample. The steps are as follows:

[0098] (SI) Prepare 10 parts of NaCl solutions with a known concentration of 0-1.8% (mass percentage); the solution with a concentration of 0 is deionized water, and it is used as a standard solution.

[0099] (S2) Put deionized water into the sample coupler; turn on the light-emitting device 1, and the light is incident on the polarization state preparation device 2 and the phase compensation system 3 (phase compensation δ≈-1.275rad), so as to satisfy the incident angle of the total reflection condition θ=93.0° is incident on the prism-sample interface, and the reflected light passes through the polarization state selection device 7, and is divided into high-frequency light and low-frequency light by the spectrum frequency division ...

Embodiment 3

[0114] This embodiment is based on the quantum weak measurement technology, and the surface plasmon sensor based on the quantum weak measurement provided in Example 1 is used to measure the binding process of rabbit IgG molecules and protein A molecules. The steps are as follows:

[0115] After standard sample calibration, we can monitor the binding process of biomolecules. Prepare a dopamine solution with a concentration of 2 mg / mL, pH=8.5, and a solvent of 10 mM tris buffer, use phosphate buffer saline as the cleaning channel (PBS, pH7.4), and use bovine serum albumin (BSA) as the blocking solution. In the optical path system in embodiment 2, the binding process of different concentrations of rabbit IgG molecules and protein A is monitored, and the schematic diagram of the test results is as follows Figure 4 shown.

[0116] During the molecular binding test, firstly, a dopamine solution was continuously passed through the flow channel, and a layer of adhesive polydopamine ...

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Abstract

The invention relates to a frequency domain quantum weak measurement biomolecule sensor and a measurement method thereof. The sensor comprises a light emitting device, a polarization state preparation device, a prism, a polarization state selection device, a spectrum frequency division device and photoelectric detection elements. A measured sample is contacted with the reflection surface of the prism, and a light beam emitted by the light emitting device is changed into polarized light through the polarization state preparation device; the polarized light enters the reflecting surface of the prism, phase difference is generated through interface reflection, polarization states are selected through the polarization state selection device, the polarized light is separated into light beams in different frequency intervals after passing through the spectrum frequency division device, the light beams are received by the photoelectric detection elements respectively, and a sample refractive index measurement result is obtained by calculating a light intensity difference value. Therefore, the concentration of sample molecules on the surface of the prism can be determined and / or the biochemical reaction process can be monitored. According to the invention, on the premise of ensuring high-sensitivity measurement of the refractive index of a biomolecule sample, the measurement stability, repeatability and resistance to environmental noise interference can be improved.

Description

technical field [0001] The invention relates to quantum optical technology, in particular to a frequency-domain quantum weak measurement biomolecular sensor and a measurement method thereof. Background technique [0002] The optical measurement method of the concentration of biomolecules generally uses the surface plasmon resonance (Surface plasmon resonance, SPR) method to measure the refractive index, or uses the ultraviolet spectrophotometric absorption method to measure the absorbance, but the above methods are costly and difficult to measure efficiently. The problem is that weak measurement methods have the characteristics of lower cost under the same sensitivity, and these measurement processes can be improved. [0003] The weak measurement method has the advantages of wide application range, sensitive measurement, and real-time in-situ sensitive measurement. According to existing work (see Zhang Y, Li D, He Y, et al. Optical weak measurement system with commonpath im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/21G01N21/01
CPCG01N21/21G01N21/01
Inventor 何永红许杨施力轩周冲琪
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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