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Method for inducing in-vitro leaf somatic embryo of Populus*euramericana Neva

A detached leaf, European and American technology, applied in the field of plant biology, can solve the problems of low regeneration rate, less budding, etc., achieve the effect of low cost, strong seedling growth, and solve the problem of germplasm degradation

Pending Publication Date: 2021-12-07
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the above problems, the present invention provides a method for inducing somatic embryos from the isolated leaves of Populus americana 107 through an indirect approach. Problems with low regeneration rate and few buds

Method used

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  • Method for inducing in-vitro leaf somatic embryo of Populus*euramericana Neva
  • Method for inducing in-vitro leaf somatic embryo of Populus*euramericana Neva
  • Method for inducing in-vitro leaf somatic embryo of Populus*euramericana Neva

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Experimental program
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Effect test

Embodiment 1

[0072] 1. Selection of explants: After falling leaves in autumn or before germination in spring, select a single plant of European and American Populus 107 with strong growth and excellent performance, and take annual branches with no pests and diseases and full buds, and use a soft brush dipped in detergent solution to carefully remove the explants. Clean the dust on the surface of the branch, rinse it with running water, put the lower end into a bucket with a 45° oblique opening, add water to 1 / 3 of the branch, and place it in a leeward sunny place for indoor hydroponics. During the cultivation period, change the water every day and control the indoor temperature At around 25°C, and provide sufficient scattered light. After germination, take the young leaves that have just unfolded as explant materials.

[0073] 2. Disinfection of explants: place the leaves on an ultra-clean workbench, disinfect with 70% alcohol for 30 seconds, rinse with sterile water for 3 times, then ster...

Embodiment 2

[0083] 1. Selection of explants: European and American Populus 107 is provided by the Shandong Academy of Forestry Sciences. After falling leaves in autumn or before sprouting in spring, annual branches with no pests and diseases and well-developed buds are collected. Rinse with clean water and cut the lower end obliquely and put them in a bucket , add water to 1 / 3 of the branches, place in a leeward sunny place for indoor hydroponics, change the water every day during the cultivation period, control the indoor temperature at about 20°C, and provide sufficient scattered light. After germination, take the young leaves that have just unfolded as explants.

[0084] 2. Disinfection of explants: place the leaves on an ultra-clean workbench, disinfect with 70% alcohol for 30 seconds, rinse with sterile water for 3 times, then sterilize with 0.1% mercury liter for 3 minutes, rinse with sterile water for 5 times, and Blot dry with sterile filter paper.

[0085] 3. Induction culture o...

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Abstract

The invention belongs to the technical field of plant biology, relates to a method for inducing the in-vitro leaf somatic embryo of Populus*euramericana Neva, and solves the problems of low regeneration rate and few buds of the Populus*euramericana Neva by respectively adopting an embryoid regeneration pathway and an adventitious bud regeneration pathway. Wherein the time of the embryoid regeneration pathway from leaf induction to callus induction of adventitious buds is more than 20 days longer than that of the adventitious bud regeneration pathway, and the number of the induced buds is more than 30 less than that of the adventitious bud regeneration pathway and is less than half of that of the adventitious bud regeneration pathway. The embryoid regeneration pathway mainly provides technical support for gene engineering (gene transformation, gene knockout and the like) and cell suspension culture. The adventitious bud regeneration pathway is mainly used for providing virus-free seedlings and carrying out rapid propagation production. By means of the method, the callus induction rate and the budding rate are both 95% or above.

Description

technical field [0001] The invention belongs to the field of plant biology technology, and in particular relates to a method for inducing somatic embryos of poplar poplar leaves in vitro. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] European and American poplar 107 (Populus×euramericana'Neva') is an excellent variety of fast-growing poplar selected and promoted by the Chinese Academy of Forestry. Become a pillar industry in the development of rural economy. Under the situation of returning farmland to forests and grasslands nationwide, 107 poplars are still in short supply. With the large-scale planting of Populus 107, due to the long-term cutting propagation and sin...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/008
Inventor 李双云乔艳辉朱士利李善文韩友吉张晓艳董玉峰姚俊修
Owner SHANDONG FOREST SCI RES INST
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