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Preparation method and application of blackberry extract

A technology of extract and blackberry, applied in the field of preparation of blackberry extract, can solve the problems of easily destroying blackberry cells, not mentioned, and high cost of the extract, and can reduce the degree of liver fibrosis, improve tissue vacuolar degeneration, and improve fat. effect of deposition

Pending Publication Date: 2021-12-03
GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problems in the application number CN201711145154.X: 1) The extraction of blackberry stock solution has been extracted four times, the operation is complicated, the cost is high, and it is not easy for industrial production; 2) The biological fermentation is easy to destroy the blackberry cells, affecting the original components and quality of blackberry ;Using high-temperature instantaneous sterilization, it is easy to destroy the active ingredients of blackberries; 4) only mentions the use of blackberry powder in food, but does not mention the use of blackberry extracts in anti-alcoholic liver damage
[0007] Application number CN201510433616.2 has technical problems: 1) Blackberries are directly placed in a homogenizer and added with 5 to 10 times of ethanol for extraction, without acidification to remove impurities, which may easily cause many impurities in the extract, incomplete extraction, and Low yield and other phenomena; 2) The cost of extracts prepared by molecular distillation is high
[0009] The technical problems in the application number CN201510018395.2: 1) The extraction method is complicated, and the extraction needs pepsin, trypsin, ultrafiltration membrane filtration, etc., and the cost is high; 2) The main component of the extract is: cyanidin-3 -glucoside, cyanidin-3-xyloside, cyanidin-3-oxalyl glucoside, cyanidin-3-malonyl glucoside, delphinidin-3-xyloside and cyanidin cyanidin-3-arabinoside, blackberry anthocyanins in the extract were not mentioned; 3) Although the extract mentioned the application in the inhibitor of liver cell oxidative damage induced by acrylamide, it did not target Application in anti-alcoholic liver injury drugs

Method used

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  • Preparation method and application of blackberry extract
  • Preparation method and application of blackberry extract
  • Preparation method and application of blackberry extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Preparation of blackberry extract

[0053] (1) Take 1000g of fresh blackberry fruit and put it into the freeze-drying machine tray, set the freeze-drying parameter as -50°C, the vacuum degree at 60Pa, the tray temperature at 20°C and dry for 48h, pulverize to 20 mesh, to obtain freeze-dried powder, for use;

[0054] (2) Precisely weigh 3.00g of freeze-dried powder, according to the material-to-liquid ratio of 1:8g / mL, add 70% methanol solution containing 0.1% formic acid as the extraction solution, extract 90min by ultrasonic method at a temperature of 40°C and a power of 300W , repeat the extraction 3 times, the extracts are combined by suction filtration, concentrated to the extract with a relative density of 1.3, for use;

[0055] (3) take the extract and freeze-dry in vacuum to obtain the blackberry extract.

Embodiment 2

[0056] Example 2 Preparation of blackberry extract

[0057] (1) Take 1000g of fresh blackberry fruit and put it into the freeze-drying machine tray, set the freeze-drying parameters as -50°C, the vacuum degree at 60Pa, the tray temperature at 20°C and dry for 45h, and pulverize to 20 mesh to obtain freeze-dried powder, which is ready for use;

[0058] (2) Precisely weigh 3.00 g of freeze-dried powder, according to the material-to-liquid ratio of 1:4 g / mL, add 60% methanol solution containing 0.1% formic acid as the extraction solution, extract 90min by ultrasonic method at a temperature of 40°C and a power of 300W , repeated the extraction 3 times, the extracts were combined by suction filtration, concentrated to an extract with a relative density of 1.1, for later use;

[0059] (3) take the extract and freeze-dry in vacuum to obtain the blackberry extract.

Embodiment 3

[0060] Example 3 Preparation of blackberry extract

[0061] (1) Take 1000g of fresh blackberry fruit and put it into the freeze-drier tray, set the freeze-drying parameters as -50°C, the vacuum degree at 60Pa, the tray temperature at 20°C and dry for 52h, and pulverize to 30 mesh to obtain freeze-dried powder, which is ready for use;

[0062] (2) Precisely weigh 3.00g of freeze-dried powder, according to the material-to-liquid ratio of 1:10g / mL, add 80% methanol solution containing 0.1% formic acid as the extraction solution, extract 90min by ultrasonic method at a temperature of 40°C and a power of 300W , repeated the extraction 3 times, the extracts were combined by suction filtration, concentrated to the extract with a relative density of 1.5, for use;

[0063] (3) take the extract and freeze-dry in vacuum to obtain the blackberry extract.

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Abstract

The invention relates to a preparation method and application of a blackberry extract. The blackberry extract is obtained by freeze-drying fresh blackberries, extracting with methanol, concentrating and drying or purifying with polyamide resin, and concentrating and drying. The effective dose of the extract is 25-100 mg / kg / d, the extract can reduce the harm of alcoholic liver injury and remarkably reduce the levels of AST, ALT, SOD and MDA in mouse serum, meanwhile, the blackberry extract can remarkably improve alcohol-induced liver injury such as mouse liver tissue hepatitis, hepatic necrosis, fatty liver and hepatic fibrosis, and the blackberry extract can be well applied to medicines, functional beverages and health-care foods for resisting alcoholic liver injury.

Description

technical field [0001] The invention relates to the fields of medicine and food, in particular to a preparation method and application of a blackberry extract. Background technique [0002] Alcohol abuse and alcohol dependence are major public health problems worldwide. With the continuous improvement of people's living standards, the number of alcohol consumption in my country is increasing year by year, and the damage to the nervous and digestive systems caused by alcoholism is also increasing. The liver is the main organ in the process of ethanol metabolism. Long-term alcohol abuse will lead to alcoholic liver damage (ALD), such as fatty liver, alcoholic hepatitis, liver fibrosis, liver cirrhosis, and also increase the incidence of liver cancer. probability. According to the World Health Organization (WHO), 2 billion people around the world drink alcohol, of which more than 7.5 million people abuse alcohol. Excessive drinking has become the main cause of liver disease. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A23L33/105A23L2/52A61P1/16A61K131/00
CPCA61K36/73A61P1/16A23L33/105A23L2/52A61K2236/17A61K2236/333A61K2236/53A61K2236/39A61K2236/51A23V2002/00A23V2200/30A23V2250/21
Inventor 肖婷郭正红骆光佳张道芳
Owner GUIZHOU MEDICAL UNIV
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