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Bone repair material and preparation method and application thereof

A bone repair and collagen technology, applied in the field of biomedical materials, can solve the problems of low biosafety, low plasticity of mechanical strength materials, and inability to distribute hydroxyapatite evenly, achieve high biological activity, promote adhesion and proliferation, The effect of accelerating blood vessel regeneration

Pending Publication Date: 2021-11-26
BEIJING BIOSIS HEALING BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] In view of the problems existing in the prior art, for example, in the bone repair material prepared by the existing method, hydroxyapatite cannot be evenly distributed into the collagen scaffold; the existing bone repair material is in powder form, and its mechanical strength and material plasticity are extremely low; and The preparation process of bone repair materials requires the use of cross-linking agents, resulting in low biosafety defects

Method used

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  • Bone repair material and preparation method and application thereof
  • Bone repair material and preparation method and application thereof
  • Bone repair material and preparation method and application thereof

Examples

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Effect test

preparation example Construction

[0068] Preparation method of bone repair material

[0069] The first aspect of the present disclosure provides a method for preparing a bone repair material, which includes the following steps:

[0070] Dissolving step: at a first temperature, preparing a dissolving solution containing collagen;

[0071] Dispersing step: dispersing hydroxyapatite in the solution, stirring evenly at the first temperature to obtain a mixed solution;

[0072] Incubation step: placing the mixed solution in an isotropic and equal pressure environment, and incubating at a second temperature to form a collagen scaffold; the hydroxyapatite particles are loaded inside and / or on the surface of the collagen scaffold, Complete the self-assembly of hydroxyapatite and collagen;

[0073] Freeze-drying step: freeze-drying the product of the incubation step to obtain the bone repair material;

[0074] Wherein, the first temperature is 2-10°C. Exemplarily, the first temperature is 3°C, 4°C, 5°C, 6°C, 8°C, ...

Embodiment 1

[0135] The present embodiment provides the preparation method of natural collagen material, comprises the steps:

[0136] (1) Cleaning pretreatment: at room temperature, scrape off the mucous membrane layer and fascia layer of the small intestine, retain the submucosa layer of the middle small intestine, and wash the material with purified water for later use.

[0137] (2) Virus inactivation: prepare a virus inactivation aqueous solution (1% (v / v) peracetic acid+24% (v / v) ethanol), soak the cleaned small intestinal submucosa material in the virus inactivation solution, The volume ratio of the small intestinal submucosa material to the solution was 1:30 (v:v), placed on a shaker, soaked at room temperature for 2 hours, and the shaker speed was 40r / min. Wash with purified water after soaking.

[0138] (3) Degreasing: the configuration concentration is 2wt% alkaline lipase solution, the volume after the virus is extinguished is soaked in the degreasing solution, the volume ratio...

Embodiment 2

[0146] This embodiment provides a method for preparing a bone repair material, using the natural collagen material prepared in Example 1 to prepare a collagen solution, including the following steps:

[0147] (1) Dissolving step: using cold PBS buffer solution to dissolve collagen at low temperature (4°C) to obtain a solution with a collagen concentration of 1% (w / v).

[0148] (2) Dispersion step: disperse hydroxyapatite (HA) in the collagen solution, and disperse and stir evenly at low temperature (4° C.) to obtain a mixed solution of HA / collagen, wherein the mass ratio of collagen to hydroxyapatite is 1: 29, 1:19, 1:14, and 1:9.

[0149] (3) Incubation step: put the HA / collagen mixed solution into the pre-cooled mold, use an isostatic pressing device (JDP-40J, Shanghai Jingsheng Scientific Instrument Co., Ltd.) to briquette, and place it in a 37°C environment for incubation After 1 hour, it was taken out from the mold to obtain the HA / collagen composite material.

[0150] ...

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Abstract

The invention belongs to the field of biomedical materials, and particularly relates to a bone repair material and a preparation method and application of the bone repair material. According to the preparation method of the bone repair material provided by the invention, hydroxyapatite particles are uniformly wrapped in a collagenous fiber three-dimensional network by utilizing self-organization of the hydroxyapatite particles and collagen, and the bone repair material with a porous structure and a moldable characteristic is prepared. The bone repair material is high in mechanical strength and cannot be dispersed and broken in the use and transportation processes; and the bone repair material has good moldability, can be cut at will according to the shape of a defect area, becomes soft when encountering water, is rich in elasticity, is suitable for bone defect filling at any site, and plays the functions of bone filling and bone tissue regeneration guiding.

Description

technical field [0001] The disclosure belongs to the field of biomedical materials, in particular, the disclosure relates to a bone repair material, a preparation method and an application of the bone repair material. Background technique [0002] Factors such as trauma, body aging, tumors, and bone diseases can all lead to bone defects or bone damage. In orthopedic clinical practice, guided tissue regeneration through bone grafting is a commonly used bone repair method. In clinical oral cavity, in order to deal with alveolar bone defect or underestimation of alveolar bone caused by tooth extraction or trauma, it is often necessary to perform restorative filling of bone in the alveolar area before oral implants. [0003] In the bone defect area, the bioactive bone repair scaffold is filled in the bone defect area. The bioactive material can regulate the microenvironment around the bone tissue and induce the life activities of cells in a controllable manner, thus improving t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/12A61L27/50A61L27/54A61L27/56A61L27/58C12P21/06C07K14/78C07K1/14
CPCA61L27/24A61L27/12A61L27/50A61L27/54A61L27/56A61L27/58C12P21/06C07K14/78A61L2430/02A61L2430/12A61L2300/412A61L2300/112A61L2300/252
Inventor 景伟李红阳赵博魏鹏飞胡苗苗
Owner BEIJING BIOSIS HEALING BIOLOGICAL TECH
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