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Matrine-based ionic liquid conotoxin polypeptide solution as well as preparation method and application thereof, and matrine-based ionic liquid conotoxin polypeptide preparation as well as preparation method and application thereof

A technology of conotoxin and ionic liquid, applied in the field of microemulsion, can solve the problems of first-pass effect, existence of liver, poor effect, etc., achieve high solubility, mild percutaneous absorption, and reduce the incidence of adverse reactions

Pending Publication Date: 2021-11-26
SHENZHEN SHINESKY BIOLOGICAL TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a matrine base ionic liquid conotoxin polypeptide solution, preparation and its preparation method and application, aiming at solving the problem of the existing polypeptide component drugs when they are taken orally. It is easy to be degraded by enzymatic reaction in gastric acid environment, and there is a first-pass effect of the liver. When it is used externally, it is also affected by the effect of the skin barrier and the effect is not good.

Method used

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  • Matrine-based ionic liquid conotoxin polypeptide solution as well as preparation method and application thereof, and matrine-based ionic liquid conotoxin polypeptide preparation as well as preparation method and application thereof
  • Matrine-based ionic liquid conotoxin polypeptide solution as well as preparation method and application thereof, and matrine-based ionic liquid conotoxin polypeptide preparation as well as preparation method and application thereof
  • Matrine-based ionic liquid conotoxin polypeptide solution as well as preparation method and application thereof, and matrine-based ionic liquid conotoxin polypeptide preparation as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Add 0.01 mol of matrine and 0.01 mol of octanoic acid into a reaction vessel filled with 20 mL of water and stir for 12 hours to obtain a matrine organic acid ion salt solution. At room temperature, 0.001 g of the conotoxin polypeptide was added into 10 mL of water and stirred evenly to obtain the conotoxin polypeptide mother liquid. Under the condition of stirring, the above matrine organic acid ion salt solution is added dropwise into the conotoxin polypeptide mother liquor to obtain the matrine base ionic liquid conotoxin polypeptide solution. At room temperature, mix the emulsifier polyvinylpyrrolidone and the auxiliary agent sorbitol fatty acid ester at a molar ratio of 1:1 and mix well and uniformly to obtain a mixed emulsifier solution. Take 4g mixed emulsifier solution, under stirring condition, add above-mentioned matrine base ionic liquid conotoxin polypeptide solution drop by drop in 4g mixed emulsifier solution, observe the phenomenon that the system changes...

Embodiment 2

[0072]Add 0.01 mol of matrine and 0.01 mol of malic acid into a reaction container filled with 20 mL of water and stir for 12 hours to obtain a matrine organic acid ion salt solution. At room temperature, 0.100 g of the conotoxin polypeptide was added to 10 mL of water and stirred evenly to obtain the conotoxin polypeptide mother liquid. Under the condition of stirring, the above matrine organic acid ion salt solution is added dropwise into the conotoxin polypeptide mother liquor to obtain the matrine base ionic liquid conotoxin polypeptide solution. At room temperature, mix 0.848 g of emulsifier polyvinyl alcohol and auxiliary agent sorbitol fatty acid ester at a molar ratio of 1:1 and mix thoroughly to obtain a mixed emulsifier solution. Take 4g mixed emulsifier solution, under stirring condition, add above-mentioned matrine base ionic liquid conotoxin polypeptide solution drop by drop in 4g mixed emulsifier solution, observe the phenomenon that the system changes from turbi...

Embodiment 3

[0082] Preparation of anti-wrinkle firming eye cream containing matrine base ionic liquid conotoxin polypeptide preparation:

[0083] Prepare according to the following mass percentages: 0.1% matrine base ionic liquid conotoxin polypeptide preparation in Example 1, 2.5% wheat germ oil, 0.5% sodium hyaluronate, 0.01% vitamin B1, 0.01% vitamin E, 2.0 % surfactant, 0.5% co-surfactant and the balance of water to obtain the anti-wrinkle firming eye cream containing matrine base ionic liquid conotoxin polypeptide preparation. And it has been carried out actual use test, this anti-wrinkle firming eye cream is applied around the eyes of the tester who has wrinkles around the eyes, the test results of the anti-wrinkle firming eye cream are as follows: Figure 5 As shown in (a) and (b), it can be seen that the anti-wrinkle effect of the eye cream is obvious after 10 minutes of use. It shows that matrine-based ionic liquid conotoxin polypeptide preparation can effectively promote the pe...

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Abstract

The invention discloses a matrine-based ionic liquid conotoxin polypeptide solution as well as a preparation method and application thereof, and a matrine-based ionic liquid conotoxin polypeptide preparation and a preparation method and application thereof. The preparation method comprises the following steps of providing conotoxin polypeptide mother liquor; adding matrine and organic acid into water to obtain a matrine organic acid ionic salt solution; and mixing the matrine organic acid ionic salt solution with the conotoxin polypeptide mother liquor to obtain the matrine -based ionic liquid conotoxin polypeptide solution. The prepared matrine-based ionic liquid conotoxin polypeptide solution is good in stability and biocompatibility, has slow-release and permeation-promoting effects, can be delivered in a transdermal way, can avoid easy degradation of polypeptide drugs in a digestive system under acidic and enzymatic reaction conditions and a first-pass effect of the liver in clinical application, and can reduce the influence of a skin barrier effect during external application.

Description

technical field [0001] The invention relates to the technical field of microemulsions, in particular to a matrine base ionic liquid conotoxin polypeptide solution, a preparation and a preparation method and application thereof. Background technique [0002] The conotoxin polypeptide is a complex short peptide mixture secreted by the venom tube of the cone snail (Conus geographus) and the poisonous glands on the inner wall of the poison sac. There are potential applications in the skin. In addition, conotoxin polypeptides are also antagonists of N receptors and NMDA receptors, especially the Con-G protein has a specific type of NMDA receptor 2B subtype, so conotoxin polypeptides are more effective than morphine in the treatment of various diseases. It is selective and will not be addictive or have side effects within the therapeutic dose range. In addition, due to its association with NMDA receptors, it has potential in the treatment of Alzheimer's and Parkinson's diseases,...

Claims

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Application Information

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IPC IPC(8): A61K38/02A61K9/107A61K47/22A61P17/06A61P25/16A61P25/28A61P25/30A61P29/00A61P37/08A61K8/02A61K8/49A61K8/64A61Q19/02A61Q19/08A61K31/4375
CPCA61K38/02A61K31/4375A61K9/1075A61K9/0014A61K47/22A61P25/28A61P25/16A61P25/30A61P17/06A61P37/08A61P29/00A61K8/64A61K8/4926A61K8/0291A61Q19/08A61Q19/02A61K2300/00
Inventor 张嘉恒余明远王振元柴记红谭兴春苏晶
Owner SHENZHEN SHINESKY BIOLOGICAL TECH CO LTD
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