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Preparation way of incompletely enzyme-digested sericin anti-oxidation and anti-initial-aging stock solution

A collagen and antioxidant technology, applied in cosmetic preparations, cosmetics, pharmaceutical formulations, etc., to stabilize and repair skin damage and have stable properties

Pending Publication Date: 2021-11-12
伊深细胞生物技术上海有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation method of an incomplete enzymatically cut sericin protein anti-oxidation and anti-aging stock solution to solve the problem that sericin protein itself does not have the ability to react with free radicals and remove it due to its stable macromolecular structure. free radicals, but incompletely hydrolyzed sericin protein has a strong ability to remove hydroxyl and superoxide free radicals; therefore, a hydrolyzed sericin protein that can maintain the stability of incompletely hydrolyzed protein components can improve its ability to scavenge free radicals ability problem

Method used

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  • Preparation way of incompletely enzyme-digested sericin anti-oxidation and anti-initial-aging stock solution
  • Preparation way of incompletely enzyme-digested sericin anti-oxidation and anti-initial-aging stock solution
  • Preparation way of incompletely enzyme-digested sericin anti-oxidation and anti-initial-aging stock solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0019] A preparation method of an incomplete enzyme-cut sericin protein anti-oxidation and anti-aging stock solution, comprising the following steps:

[0020] Step 1: Dissolve 100g of sericin powder in 1L of distilled water to make A solution with a concentration of 0.1g / ml;

[0021] Step 2: Dissolve 100g of protease in 1L of distilled water to prepare a B solution with a pH of 7.0 and a concentration of 0.1g / ml;

[0022] Step 3: Dissolve 10g of trehalose powder and 10g of hyaluronic acid in 1L of distilled water at a ratio of 1:1 to prepare a C solution with a concentration of 0.01g / ml;

[0023] Step 4: Heat solution A to 50 degrees, add solution B according to the ratio of 1 / 100 of solution A (the ratio of enzyme and protein is 1:100) to maintain the temperature, and test the pH value to 7, if the condition changes and Use NAHO to adjust the pH value to maintain at 7;

[0024] Step 5: After two hours of reaction, heat the solution to 80-100 degrees to destroy the protease,...

Embodiment example 2

[0026] In this implementation case, the concentration ratio of multiple groups of enzymes and proteins and the reaction time of multiple groups are set, and their clearance rates are compared. For the results, please refer to Figure 1-2 , where the scavenging rate is calculated using the free radical (OH) scavenging ability determination method.

[0027] Depend on figure 1 It can be seen that the optimum condition for protease hydrolysis is a solution with a concentration of 0.1 g / ml, a reaction temperature of 50 degrees Celsius, and a hydrolysis time of 2 hours, and the antioxidant capacity (that is, scavenging free radicals ( OH) ability) is the strongest.

Embodiment example 3

[0029] In this implementation case, the amino acid composition of sericin peptide incompletely hydrolyzed by flavor protease is tested, and compared with the data after adding stabilizer C solution, the results can be found in image 3 .

[0030] Depend on image 3 It can be seen that even if the high temperature destroys the hydrolytic enzyme to stop the reaction, some hydrolytic enzymes will continue to hydrolyze with sericin. After the solution was left to stand for 1 hour, it was found that the substance of the hydrolytic protease had changed through mass spectrometry, and the best free radical scavenging could not be achieved. stable state. Therefore, adding 1% trehalose and hyaluronic acid stock solution (1:1) after heating the original solution to destroy the enzyme can protect the hydrolyzed protein peptide from hydrolysis. Trehalose is a stable non-reducing disaccharide with very stable properties and can It forms a special protective film on the cell surface under ...

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Abstract

The invention discloses a preparation way of an incompletely enzyme-digested sericin anti-oxidation and anti-initial-aging stock solution. The preparation way comprises the following steps of: dissolving 100g of sericin powder in 1L of distilled water to prepare a solution A with the concentration of 0.1 g / ml; dissolving 100g of protease into 1L of distilled water to prepare a solution B with the pH value of 7.0 and the concentration of 0.1 g / ml; dissolving 10g of trehalose powder and 10g of hyaluronic acid in 1L of distilled water according to the ratio of 1: 1 to prepare a solution C with the concentration of 0.01 g / ml; heating the solution A to 50 DEG C, adding a solution B (the ratio of enzyme to protein is 1: 100) according to the proportion of 1 / 100 of the solution A, maintaining the temperature, testing the PH value to be 7, and if conditions change, regulating the PH value to be maintained to be 7 through NAHO; and after reacting for two hours, heating the solution to 80-100 DEG C to destroy the protease, terminating the hydrolysis reaction of enzyme, cooling to the room temperature, and adding 1 / 10 of the solution C for stabilizing. According to the preparation way, protein components which are not completely hydrolyzed can be kept stable, so that the optimal stable state of scavenging free radicals can be achieved.

Description

technical field [0001] The invention relates to the technical field of hydrolyzed sericin, in particular to a method for preparing an anti-oxidation and anti-aging stock solution of incomplete enzyme-cut sericin. Background technique [0002] Free radicals trigger oxidation, and oxidation occurs outside the human body. Light and air are likely to produce active oxidases and promote the activation of tyrosine, causing wrinkles, spots, darkening and aging of the skin, and the possibility of skin cancer. Therefore, removing free radicals from the skin epidermis can effectively prevent the initial reaction of aging and achieve the effect of anti-aging. [0003] Sericin itself does not have the ability to react with free radicals and scavenge free radicals because of its stable macromolecular structure, but the incomplete hydrolysis of sericin protein has a strong ability to remove hydroxyl and superoxide free radicals; Hydrolyzed protein components Maintain stable hydrolyzed se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/64A61K8/60A61K8/73A61Q19/08
CPCA61K8/64A61K8/60A61K8/735A61Q19/08A61K2800/805A61K2800/52
Inventor 刘牧青
Owner 伊深细胞生物技术上海有限公司
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