Preparation method and application of conductive polymer nanowire biosensor

A conductive polymer and biosensor technology, which is applied in the direction of instruments, scientific instruments, and electrochemical variables of materials, can solve the problems of cumbersome processing and expensive instruments, and achieve good conductivity, large specific surface area, and predictable surface area control effect

Pending Publication Date: 2021-11-09
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing electrochemical biosensors for pesticide residue detection still have disadvantages such as cumbersome sample pretreatment process and expensive instruments.

Method used

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  • Preparation method and application of conductive polymer nanowire biosensor
  • Preparation method and application of conductive polymer nanowire biosensor
  • Preparation method and application of conductive polymer nanowire biosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Weigh 10 mg of chondroitin sulfate and add it to 5 mL of phosphate buffer solution (pH=6.86), and dissolve it ultrasonically to obtain a mixed solution, then add 52.5 μL of pyrrole, dissolve it ultrasonically, prepare a deposition solution, and use the current-time curve (i-t) method to deposit The voltage was set to 0.75V, the sampling interval was 0.1s, the deposition time was 600s, and the static time was 1s to prepare conductive polymer nanowires.

[0045] Use a pipette to pipette 1.25 mL of phosphate buffer solution with a pH of 7.4, add 0.1917 g of 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 0.0287 g of N- Hydroxysuccinimide (NHS) is mixed homogeneously, obtains catalyst, wherein 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride concentration is 0.8mol / L, N-hydroxysuccinimide The amine concentration is 0.2 mol / L.

[0046] Combine the catalyst and concentration to 2 x 10 -6 The mol / L acetamiprid aptamer was mixed according to the...

Embodiment 2

[0048] Same as Example 1, the only difference is that the deposition voltage is 0.7V.

Embodiment 3

[0050] Same as Example 1, the only difference is that the deposition voltage is 0.8V.

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Abstract

The invention discloses a preparation method and application of a conductive polymer nanowire biosensor, and belongs to the field of electrochemical biosensors. The preparation method of the biosensor comprises the following steps: adding chondroitin sulfate into a phosphate buffer solution to form a mixed solution, adding pyrrole, carrying out ultrasonic dissolution to prepare a deposition solution, and preparing a conductive polymer nanowire through an electrochemical method; mixing a catalyst and a DNA aptamer to prepare a hatching solution; dispensing the incubation liquid on the conductive polymer nanowire, and incubating to obtain the conductive polymer nanowire biosensor. According to the method, different DNA aptamers can be selected to develop the biosensor suitable for detecting different pesticide residues, and the method has the advantages that the operation is simple, and the rapid and accurate detection of the pesticide residues can be realized.

Description

technical field [0001] The invention relates to a preparation method and application of a conductive polymer nanowire biosensor, belonging to the field of electrochemical biosensors. Background technique [0002] In recent years, both at home and abroad have increased the research and development of pesticides to a certain extent, and have continuously developed new pesticide products. However, the wide application of pesticides has caused serious environmental problems and food safety issues, which has also become a constraint on the export of agricultural products in my country. a bottleneck. [0003] Therefore, rapid detection and pesticide residue detection methods that do not require pretreatment are required. In recent years, research on electrochemical analysis has attracted more and more attention, but there are still problems such as low sensitivity and poor stability, which restrict the development of electrochemical detection. . Electrochemical biosensor is a new...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3277G01N27/3278G01N27/48
Inventor 张德成王家胜惠妮
Owner QINGDAO AGRI UNIV
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