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Composition and kit for detecting helicobacter pylori in oral cavity

A Helicobacter pylori and composition technology, applied in the field of in vitro detection of pathogenic bacteria, can solve the problems of cumbersome detection steps, inability to find product information, inconvenient self-inspection by ordinary people, etc., and achieve simple, accurate and rapid detection Effect

Pending Publication Date: 2021-11-09
修正生物医药(杭州)研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the product information is no longer available
[0015] 2) The detection steps are cumbersome
It is inconvenient for the general population to self-check
[0016] In summary, there are many problems in the existing technology or products, which are not suitable for self-examination of suspected Helicobacter pylori symptoms by consumers

Method used

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  • Composition and kit for detecting helicobacter pylori in oral cavity
  • Composition and kit for detecting helicobacter pylori in oral cavity
  • Composition and kit for detecting helicobacter pylori in oral cavity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation of detection composition

[0046] Dissolve 0.027 g of anhydrous dipotassium hydrogen phosphate and 0.002 g of sodium hydroxide in 100 mL of distilled water and mix well to prepare a buffer solution. Add 2 g of urea, 0.02 g of bromothymol blue and 0.2 g of methyl p-hydroxybenzoate to the above buffer solution to completely dissolve and mix the components. After the above aqueous solution is filtered with a 0.22 μm sterile filter membrane, the detection composition is obtained.

[0047]The contents of each component in the detection composition are: urea 2% w / v, bromothymol blue 0.02% w / v, dipotassium hydrogen phosphate 0.027% w / v, sodium hydroxide 0.002% w / v, p-hydroxy Methyl Benzoate 0.2% w / v. The pH of the test composition was 6.5.

Embodiment 2

[0048] Example 2 Preparation of Helicobacter pylori detection kit

[0049] The detection composition prepared in Example 1 was filled into a squeeze plastic bottle.

[0050] Prepare 95% ethanol and fill it into another squeeze plastic bottle.

[0051] Prepare the detection device and sampling device.

[0052] Thereby the Helicobacter pylori detection kit was prepared.

[0053] Oral Sample Sampling Device The length of the toothbrush is 7.5 cm.

[0054] The size of the detection device is 6cm*2cm*0.3cm, including a hole with a radius of 0.5cm and a depth of 0.2cm. The detection device is obtained by cutting a plastic plate and then punching it.

Embodiment 3

[0055] Example 3 Efficacy Verification

[0056] Samples were taken from 6 patients with chronic gastritis who had been tested by carbon-13 breath method, and were tested using the above-mentioned test kits and gastric Helicobacter pylori (HP) test strips produced by Guangzhou Beisiqi Company.

[0057] Besky products are operated according to their operating instructions.

[0058] The detection operation of the detection kit of the present invention is as follows:

[0059] 1. Wait for the temperature of the test kit to rise to room temperature. Use a sampling device to scrape the tongue coating from the middle and back of the upper surface of the tongue, and put the tongue coating sample into the reaction well.

[0060] 2. Squeeze out one to several drops of 95% ethanol into the reaction well until the sample is covered. Agitate slightly with a sampling device and wait 5 to 10 minutes.

[0061] 3. Squeeze out a few drops of the above-mentioned detection composition into the...

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Abstract

The invention provides a detection composition for detecting helicobacter pylori. The composition contains urea, dipotassium phosphate, sodium hydroxide, methyl p-hydroxybenzoate, bromothymol blue and distilled water. The invention also provides a kit containing the composition and used for detecting helicobacter pylori. The kit comprises an oral cavity sampling device, a detection device, an extrusion type plastic bottle filled with the composition and an extrusion type plastic bottle filled with 95% v / v ethanol. The kit has the following technical effects that the kit is simple and convenient to operate, is suitable for general consumers without professional knowledge and equipment, and can be used for accurately and quickly detecting helicobacter pylori in an oral cavity sample.

Description

technical field [0001] The invention relates to in vitro detection of pathogenic bacteria, in particular to a composition and a kit for detecting Helicobacter pylori in oral cavity. Background technique [0002] By 2015, the global infection rate of Helicobacter pylori was 60.3%, and the overall infection rate in China was over 50%. In particular, there is a higher infection rate among young people. It is generally believed that socioeconomic factors are one of the important reasons affecting the infection rate of Helicobacter pylori. Low-income families are more likely to carry Helicobacter pylori infection. Low education, living in rural areas, crowded families and drinking water pollution sources will increase the infection rate of Helicobacter pylori. The economic development and the improvement of living standards will reduce the infection rate of Helicobacter pylori. my country's national conditions are existing and there will still be a large number of people whose...

Claims

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Application Information

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IPC IPC(8): C12Q1/58C12Q1/04C12R1/01
CPCC12Q1/58C12Q1/04
Inventor 张弨
Owner 修正生物医药(杭州)研究院有限公司
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