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Method for producing l-lactic acid by using distilled grains

A technology of liquor and waste residues, which is applied in the field of advanced processing and fermentation of agricultural and sideline products to prepare L-lactic acid, can solve the problems of low efficiency of enzymatic hydrolysis of liquor and high cost of reuse of liquor waste residues, so as to improve selection adaptability, increase energy consumption, The effect of reducing energy consumption

Active Publication Date: 2022-06-21
LUZHOU LAOJIAO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is the low efficiency of enzymatic hydrolysis of liquor discarded grains in the prior art, and the high cost of reuse of liquor discarded grains

Method used

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  • Method for producing l-lactic acid by using distilled grains
  • Method for producing l-lactic acid by using distilled grains

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Experimental program
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Effect test

preparation example Construction

[0049] The preparation method of the above-mentioned mixed bacteria enzyme liquid, comprises the following steps:

[0050] a. Take samples from pit mud, discarded grains, and the surrounding soil of the winery, screen and enrich the screening medium with liquor discarded grains as the carbon source, and obtain bacterial samples after separation and purification; after the bacterial samples are activated, use The solid fermentation medium is fermented and cultivated, and the inoculum of the activated bacteria sample is 9-11% of the solid fermentation medium, then it is leached, suction filtered, and centrifuged to obtain bacterial enzyme liquid A, which is used for liquor The enzymatic hydrolysis of the discarded grains is screened to obtain H bacteria, and the H bacteria enzyme liquid is obtained by fermentation, and the enzyme activity of the H bacteria enzyme liquid is 5.0-5.8IU / ml;

[0051] b. Take samples from pit mud, discarded grains, and the surrounding soil of the wine...

Embodiment 1

[0088] A. Mix the discarded grains of liquor with tap water according to a solid-to-liquid ratio of 1:3 g / ml, soak for 4 hours, wet pulverize the infiltrated discarded grains to 200 mesh, and filter to obtain the crushed discarded grains;

[0089] B. PEG-8000 is mixed with mixed bacterial enzyme solution (H bacterial enzyme solution: L bacterial enzyme solution: M bacterial enzyme solution is 16: 3: 1) at a ratio of 0.004: 1 g / ml to obtain a mixed solution;

[0090] C. Mix the pulverized and discarded grains obtained in step A and the mixed solution obtained in step B according to the ratio of 1:10g / ml, at 50°C, under the condition of 140rpm, constant temperature vibration for 72h enzymolysis, suction filtration to obtain the initial sugar solution, at 50°C The initial sugar solution was evaporated under reduced pressure to a reducing sugar concentration of 60 g / L to obtain a concentrated sugar solution;

[0091] D. Adjust the pH of the concentrated sugar solution to 6.5 with ...

Embodiment 2

[0094] A. Mix the discarded grains of liquor with tap water according to a solid-to-liquid ratio of 1:3 g / ml, soak for 4 hours, wet the infiltrated grains of grains to 250 mesh, and filter to obtain crushed grains of grains;

[0095] B. PEG-8000 is mixed with mixed bacterial enzyme solution (H bacterial enzyme solution: L bacterial enzyme solution: M bacterial enzyme solution is 15: 4: 1) at a ratio of 0.005: 1 g / ml to obtain a mixed solution;

[0096] C. The pulverized and discarded grains obtained in step A are mixed with the mixed solution obtained in step B according to the ratio of 1:10g / ml, at 50°C, under the condition of 140rpm, constant temperature vibration for 72h enzymolysis, suction filtration to obtain primary sugar liquid, at 52°C The initial sugar solution was evaporated under reduced pressure to a reducing sugar concentration of 65 g / L to obtain a concentrated sugar solution;

[0097] D. Adjust the pH of the concentrated sugar solution to 6.5 with CaO, then add...

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Abstract

The invention discloses a method for producing L-lactic acid by using distilled grains of liquor, and belongs to the field of L-lactic acid preparation by advanced processing and fermentation of agricultural and sideline products. The method of producing L-lactic acid from liquor discarded grains includes: infiltration of discarded grains, ultrafine pulverization, preparation of mixed enzyme solution, addition of polyethylene glycol, enzymatic hydrolysis of discarded grains, concentration of dilute sugar solution, L-lactic acid fermentation, etc. Step, the present invention provides a more efficient method for the enzymatic hydrolysis of liquor waste grains; the use of ultrafine pulverization of liquor waste grains can reduce the introduction of pretreatment chemical substances on the premise of ensuring a high enzymatic hydrolysis reducing sugar yield, Furthermore, the process cost is reduced; the mixed bacteria enzyme solution adopted has strong stability, and the combined use has a significant mutual effect on enzymolysis; adopting the method of the present invention can produce 0.56-0.58g of reducing sugar per gram of dry discarded grains, which can produce 0.48‑0.49gL‑lactic acid. The process of the invention has good stability and low cost, and can effectively solve the problems of low efficiency of enzymatic hydrolysis of distilled grains and high cost of reusing distilled grains in the prior art.

Description

technical field [0001] The invention belongs to the field of L-lactic acid prepared by deep processing and fermentation of agricultural and sideline products, and relates to a method for producing L-lactic acid by discarding lees of liquor. Background technique [0002] Discarded grains in brewing are the main by-products of solid-state liquor brewing. According to the production process of solid-state liquor, about 3 tons of discarded grains will be produced for every 1 ton of liquor produced. Upgrading and transformation, the output of brewing waste will increase year by year. If such a huge amount of brewing waste is directly discharged into the environment without proper treatment, it will not only cause environmental pollution, but also cause waste of resources. The total amount of carbohydrates in the brewed grains, including starch, cellulose and hemicellulose, is 65-70%, and the polysaccharide can be degraded by processing, which can be used as a good carbon source ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P19/02C12P7/56C12N1/20C12N1/14C12N1/02C12R1/685C12R1/785C12R1/885C12R1/07
CPCC12P39/00C12P19/02C12P7/56C12N1/20C12N1/14C12N1/02
Inventor 雷翔云邓波熊燕飞李勇敖灵丁海龙彭远松马卓宋川
Owner LUZHOU LAOJIAO CO LTD
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