Electromagnetic coupling fermentation Streptomyces lavendulae var. hainanensis new var. variant microorganism biological battery

A bio-battery, electromagnetic coupling technology, applied in the direction of using electricity/wave energy to treat microorganisms, microorganisms, biochemical fuel cells, etc.

Active Publication Date: 2021-11-09
陕西麦可罗生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the prior art Streptomyces lilacaceae Hainan variant ( Streptomyces lavendulae var. hainanensis new var. ) problems such as thin lawn, decreased quality of lawn after passage, and low content of Zhongshengmycin. The present invention provides an electromagnetically coupled fermented Streptomyces lilacinus Hainan variant microbial bio-battery, which solves the problem of Zhongshengmycin in existing strains. low content technical issues

Method used

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  • Electromagnetic coupling fermentation Streptomyces lavendulae var. hainanensis new var. variant microorganism biological battery
  • Electromagnetic coupling fermentation Streptomyces lavendulae var. hainanensis new var. variant microorganism biological battery
  • Electromagnetic coupling fermentation Streptomyces lavendulae var. hainanensis new var. variant microorganism biological battery

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Streptomyces lilacinus was inoculated in the anode area of ​​MFC, the anode inoculum was glucose (AR) 1.5%, cold-pressed soybean meal (IR) 2.0%, corn flour (FR) 0.15%, corn starch (FR) 1.5%, NH 4 Cl(AR) 0.45%, NaCl(AR) 0.35%, MgSO 4 ·7H 2 O(AR)0.03%, KH 2 PO 4 (AR) 0.03%, light CaCO 3 (AR) 0.04%, tap water preparation, the pH of the shake flask seed medium before digestion is 6.5-7.5, the fermentation temperature of Zhongshengmycin is 25°C, the ventilation rate is 600-650L / min, the stirring speed is 120rmp, light The inoculation amount of Streptomyces purple was 10%, the feeding amount was 3mL / L h, the cathodic liquid was the wastewater from the fermentation workshop of Shaanxi Mycobasugamycin, the initial COD concentration of the wastewater was 650mg / L, and the ammonia nitrogen concentration was 65mg / L. In this way, the MFC of Streptomyces lilacinus was constructed.

Embodiment 2

[0030] Streptomyces lilacinus was inoculated in the anode area of ​​the MFC, and the anode inoculum was glucose (AR) 1.5%, cold-pressed soybean meal (IR) 2.0%, corn flour (FR) 0.15%, corn starch (FR) 1.5%, NH 4 Cl(AR) 0.45%, NaCl(AR) 0.35%, MgSO 4 ·7H 2 O(AR)0.03%, KH 2 PO 4 (AR) 0.03%, light CaCO 3 (AR) 0.04%, tap water preparation, the pH of the shake flask seed medium before digestion is 6.5-7.5, the fermentation temperature of Zhongshengmycin is 25°C, the ventilation rate is 600-650L / min, the stirring speed is 120rmp, light The inoculum amount of Streptomyces violaceum was 10%, and the feeding amount was 3mL / L·h. In the absence of an external electric field, the content of Zhongshengmycin F increased first, then remained basically unchanged, and then decreased with the extension of fermentation time. The maximum value is reached after 24 hours. The content of mitochondrial enzyme I increased slowly and then decreased with the prolongation of fermentation time, and rea...

Embodiment 3

[0033] Streptomyces lilacinus was inoculated in the anode area of ​​the MFC, and the anode inoculum was glucose (AR) 1.5%, cold-pressed soybean meal (IR) 2.0%, corn flour (FR) 0.15%, corn starch (FR) 1.5%, NH 4 Cl(AR) 0.45%, NaCl(AR) 0.35%, MgSO 4 ·7H 2 O(AR)0.03%, KH 2 PO 4 (AR) 0.03%, light CaCO 3 (AR) 0.04%, tap water preparation, the pH of the shake flask seed medium before digestion is 6.5-7.5, the fermentation temperature of Zhongshengmycin is 25°C, the ventilation rate is 600-650L / min, the stirring speed is 120rmp, light The inoculum amount of Streptomyces violaceum was 10%, the feeding amount was 3mL / L·h, and the content of Zhongshengmycin changed with the fermentation time under the action of an external electric field of 20mA, see image 3 ,From image 3 It can be seen from the figure that when 20mA direct current is applied, the content of Zhongshengmycin F increases firstly and then basically remains unchanged with the prolongation of fermentation time, and re...

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Abstract

The invention relates to an electromagnetic coupling fermentation Streptomyces lavendulae var. hainanensis new var. variant microorganism biological battery. The electromagnetic coupling fermentation Streptomyces lavendulae var. hainanensis new var. variant microorganism biological battery comprises an anode carbon rod, a cathode carbon rod, a shake flask culture medium, a fixed-value resistor, a variable resistor, a proton exchange membrane, a wastewater configuration liquid, an external electric field, a workstation, a pH sensor, a dissolved oxygen sensor, a stirrer, an oxygenation device and an external magnetic field, wherein the anode carbon rod is inoculated with the Streptomyces lavendulae var. hainanensis new var. variant. By adopting the electromagnetic coupling environment, the production titer of the Streptomyces lavendulae can be improved, and meanwhile, wastewater generated in the fermentation production process of biopesticide can be degraded.

Description

technical field [0001] The invention belongs to the field of microbial pharmacy, and in particular relates to an electromagnetically coupled fermented Streptomyces lilacinus varietal microbial biological battery and its application. Background technique [0002] Zhongshengmycin (Zhongshengmycin) belongs to the N-glycoside agricultural antibiotics. It contains 6 effective components, and each component is sequentially increased by a β-lysine. It is active against bacteria, yeast and filamentous fungal diseases. The biological bactericide formed by Zhongshengmycin through processing and preparation is an environmental resource protection type biological bactericidal treatment drug. Good inhibitory or effective inactivation effect, Zhongshengmycin is widely used in agriculture, mainly to control rice thin strip disease, stripe disease, bacterial blight, black rot of Chinese cabbage, soft leather disease, green pepper red blight diseases, cucumber lavender canker, ginger blast,...

Claims

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Application Information

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IPC IPC(8): C12P19/28C12N13/00H01M8/16C02F3/00C12R1/56
CPCC12P19/28C12N13/00H01M8/16C02F3/005Y02E60/50
Inventor 潘忠成沈伟杰赵珺涵翁婧陈豪师维李蒲民
Owner 陕西麦可罗生物科技有限公司
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