Preparation method and application of biosensor based on chemical resistance for construction site

A biosensor, construction site technology, applied in scientific instruments, material electrochemical variables, instruments, etc., can solve the problems of high reagent cost, long detection time, complex multi-step nucleic acid extraction process, etc., and achieve the effect of rapid process

Pending Publication Date: 2021-11-02
CHINA MCC17 GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have one or more of the following limitations: high cost of reagents, complex multi-step process for nucleic acid extraction, need for highly trained personnel, and long detection times

Method used

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  • Preparation method and application of biosensor based on chemical resistance for construction site
  • Preparation method and application of biosensor based on chemical resistance for construction site
  • Preparation method and application of biosensor based on chemical resistance for construction site

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Embodiment 1

[0029] A method for preparing an electrochemical biosensor based on chemical resistance, comprising the following steps:

[0030] S1. Electrode preparation: use standard photolithography to print the electrode on the substrate, and then use electron beam evaporation to deposit 20nm / 180nm Cr / Au;

[0031] S2.Si / SiO 2 Electrode Cleaning: Bare Si / SiO 2 Electrodes were ultrasonically pretreated with acetone and N 2 cleaning to make SiO 2 Hydrophilic surface;

[0032] S3. Functionalization of rGO devices: non-covalent functionalization of reduced graphene oxide electrodes via linker molecules and sugars;

[0033] S4. Lectin modification of rGO devices: The rGO device modification with conA lectin should follow the procedure of rGO device functionalization.

[0034] S2 includes the following steps:

[0035] S201. Bare Si / SiO 2 The electrode was pretreated with acetone ultrasonically, and then washed with piranha solution. The composition of piranha solution and the proportion ...

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Abstract

The invention discloses a preparation method and application of a biosensor based on chemical resistance for a construction site, and belongs to the field of water environment detection. The method comprises the following steps: S1, preparing an electrode: printing the electrode on a substrate by using a standard photoetching technology, and then evaporating and depositing Cr / Au of 20nm / 180nm by using an electron beam; S2, cleaning the Si / SiO2 electrode: carrying out acetone ultrasonic pretreatment and N2 cleaning on the exposed Si / SiO2 electrode, so that the SiO2 surface is hydrophilic; S3, functionalizing an rGO device: carrying out non-covalent functionalization on the reduced graphene oxide electrode and saccharides through connecting molecules; and S4, modifying the rGO device by using agglutinin: modifying the rGO device by using the conA agglutinin, wherein the rGO device functionalization program needs to be followed. The method is rapid, simple and reliable, and a detection platform is used for detecting interaction of the whole-cell bacterial agglutinin-carbohydrate in the water environment. The method can be used to distinguish bacterial from viral infections, and sort antibiotics according to curative effects.

Description

technical field [0001] The invention relates to the technical field, in particular to a preparation method and application of a chemical resistance-based biosensor for a construction site. Background technique [0002] Water is the source of life, and life activities cannot do without water. Drinking water health is related to everyone's health. In the construction industry, if the construction site is located in a remote area and there is no direct drinking water, the drinking water in the field must be free of bacteria and viruses to prevent drinking water poisoning. At present, microbial diseases caused by pathogenic microorganisms such as bacteria and viruses are the main cause of death. According to a recent report by the Centers for Disease Control and Prevention (CDC), at least 10 million people around the world are infected with antibiotic-resistant antibiotics every year. bacteria, at least 103,000 people die each year directly from these infections. Diagnostic te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327
CPCG01N27/30G01N27/3275
Inventor 虞文明王稷汪亚伟倪铭列
Owner CHINA MCC17 GRP
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