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Application of mPGES-2 as drug target for preventing and/or treating kidney diseases

A kidney disease, drug technology, applied in the field of biomedicine

Active Publication Date: 2021-10-26
XUZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no clinically specific drug for acute kidney injury, and the development of drugs or targets for renal protection is a hot and difficult point in current research

Method used

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  • Application of mPGES-2 as drug target for preventing and/or treating kidney diseases
  • Application of mPGES-2 as drug target for preventing and/or treating kidney diseases
  • Application of mPGES-2 as drug target for preventing and/or treating kidney diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Biochemical Index Determination

[0032] Detection of BUN and SCr: Blood was collected before killing the mice, and centrifuged at 3000 g for 15 minutes. The separated supernatant serum was collected and operated according to the instructions (BUN detection kit from Nanjing Jiancheng Biotechnology Co., Ltd., SCr kit from Bioassays Systems).

Embodiment 2

[0033] Example 2 H&E staining on renal histopathological observation

[0034] Specific experimental methods include:

[0035] 1. Paraffin section preparation

[0036] (1) Fixation of tissue samples: take the renal cortex tissues of each group of mice in Example 1, fix them at room temperature in 4% paraformaldehyde for 24 hours, wrap them in gauze, mark them, and wash them with running water overnight;

[0037] (2) Dehydration and transparency: put the dehydration box into the dehydrator and carry out dehydration with gradient alcohol successively, 75% alcohol 4h, 85% alcohol 2h, 90% alcohol 2h, 95% alcohol 1h, absolute ethanol 1 30min, absolute ethanol II 30min, alcohol benzene 5-10min, xylene I 5-10min, xylene II 5-10min;

[0038] (3) Wax immersion and embedding: Melt paraffin wax I at 65° for 1 hour, melt paraffin wax II at 65° for 1 hour, and melt paraffin wax III at 65° for 1 hour. Embed the wax-soaked tissues in the embedding machine. Put the melted wax into the embe...

Embodiment 3

[0047] Example 3 TUNEL staining to observe renal tubular cell apoptosis.

[0048] (1) Fixing: Take out the frozen section and return it to room temperature. Soak in tissue fixative solution or 4% paraformaldehyde (prepared in fresh PBS), and fix at room temperature for 30 minutes.

[0049] (2) Washing: Gently blot the excess liquid, submerge the slices in PBS or HBSS, and incubate at room temperature for 10-15 minutes.

[0050] Repeat this step once, then gently blot up excess liquid. At this time, a paraffin pen or a hydrophobic pen can be used to trace the outline of the sample distribution around the sample for subsequent operations. During the experiment, do not let the sample dry, the processed sample should be kept moist in the wet box.

[0051] (3) Permeabilization: soak in PBS containing 0.3% Triton X-100, and incubate at room temperature for 30 minutes for permeabilization.

[0052] (4) Washing: Submerge the washed sample in PBS or HBSS for 2-3 times, and gently b...

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PUM

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Abstract

The invention discloses an application of mPGES-2 as a drug target for preventing and / or treating kidney diseases. The invention proposes that the mPGES-2 is the cis-platinum-induced acute kidney injury drug target for the first time. Experiments show that mPGES-2 knockout can significantly reduce the levels of urea nitrogen and creatinine in the serum of a mouse with cis-platinum-induced acute kidney injury, significantly reduce the renal tissue form injury of the mouse with the cis-platinum-induced acute kidney injury and significantly improve the mitochondrial function in the kidneys of the mouse with acute kidney injury and reduce cell apoptosis. The results show that the cis-platinum-induced acute kidney injury can be reduced by mPGES-2 knockout, so that the mPGES-2 can be used as the target for preventing and / or treating the cis-platinum-induced acute kidney injury, and the mPGES-2 has very important significance on drug development and prevention and / or treatment of the diseases in the future.

Description

technical field [0001] The invention specifically relates to the application of mPGES-2 (microsomal prostaglandin E synthase-2) as a drug target for treating and / or preventing cisplatin-induced acute kidney injury, and belongs to the technical field of biomedicine. Background technique [0002] Acute kidney injury (AKI) has become a major public health problem, affecting millions of patients worldwide and leading to decreased survival and accelerated progression of underlying chronic kidney disease (CKD). Acute kidney injury can be caused by direct or indirect kidney injury, including various forms of shock, severe heart and liver disease, ureteral obstruction, nephrotoxic drugs and toxins, etc. Platinum antineoplastic drugs are widely used antineoplastic drugs for the clinical treatment of a variety of malignant tumors, and their use is closely associated with a significant increase in severe nephrotoxicity. About 1 / 3 of patients receiving cisplatin can develop acute kidne...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N9/90A61K45/00A61P13/12
CPCC12Q1/6883C12N9/90A61K45/00A61P13/12C12Y503/99003C12Q2600/158C12Q2600/136
Inventor 钟丹丹权玲玲胡成孙莹
Owner XUZHOU MEDICAL UNIV
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