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Non-specific immune preparation for culturing Chinese mitten crab and use method thereof

A technology of non-specific immunity and Eriocheir sinensis, which is applied in the fields of application, fish farming, climate change adaptation, etc., can solve the problem of non-specific immune effect not obvious, increase the body's anti-stress ability, and the anti-stress effect is not obvious, etc. problems, to achieve the effects of improving anti-stress ability and breeding effect, improving immune protection rate, and using simple and easy-to-operate methods

Inactive Publication Date: 2021-10-26
天津市动物疫病预防控制中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many functional studies and application techniques on a single type of non-specific immune substances. The play of animal immunity plays an important regulatory role, which can significantly improve the hemolymph phagocytic activity, serum lytic activity, acid phosphatase, alkaline phosphatase, serum The activity of phenol oxidase and superoxide dismutase can promote the immune function of the body; however, microbial polysaccharides do not have the effect of increasing the body's anti-stress ability
(2) Chinese herbal medicines and their extracts can significantly improve the non-specific immunity of carp, Jian carp, and heterogeneous gibel carp, such as lysozyme activity and white blood cell function, and have obvious antibacterial effects. The use will not lead to drug resistance or the accumulation of residues in the drug, and is beneficial and harmless to humans or other animals, showing its potential as an immune enhancer in aquatic animal farming; however, the anti-stress effect is not obvious
(3) Nutritional factor anti-stress substances (including vitamin C, vitamin E, vitamin K, vitamin A, etc.) can significantly improve the anti-stress ability of farmed animals, enhance the disease resistance of fish, and maintain the normal metabolic function of farmed animals and optimal health; however, its non-specific immune effects are not significant
Therefore, non-specific immune compound preparations may become biologically active substances to replace traditional antibiotics, but there is still a lack of research on the impact of non-specific immune compound preparations on the immune response of cultured Eriocheir sinensis. Microbial polysaccharides, Chinese herbal medicines, anti-stress substances and other non-specific immune substances are compounded as raw materials, but the prepared non-specific immune compound preparations often cannot fully utilize the advantages of each raw material component, and the application effect is often limited, and different components and ratios are prepared. The prepared compound preparations are very different

Method used

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  • Non-specific immune preparation for culturing Chinese mitten crab and use method thereof
  • Non-specific immune preparation for culturing Chinese mitten crab and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A non-specific immune preparation for cultivating mitten crabs, characterized in that each kilogram of the immune preparation contains the following powdered active ingredients: 0.075 kg of β-glucan, 0.1625 kg of three yellow powder, vitamin C Phosphate 0.1125kg, peptidoglycan 0.125kg, allicin 0.025kg. That is, the five raw materials are all powders with a particle size greater than 80 mesh.

[0022] Accurately weigh the above-mentioned active ingredients according to the formula, use wheat secondary flour with a particle size greater than 80 mesh as a carrier, and mix according to the routine to prepare the non-specific immune preparation for cultivating Chinese mitten crabs of the present invention.

[0023] A method for using non-specific immune preparations for cultivating mitten crabs, characterized in that the specific implementation steps are as follows:

[0024] (1) The immune preparation was added to the mitten crab feed at a ratio of 1:400 and mixed evenly, a...

Embodiment 2

[0027] A non-specific immune preparation for cultivating Chinese mitten crabs, characterized in that each kilogram of the immune preparation contains the following active ingredients in weight: 0.075 kg of β-glucan, 0.1625 kg of three yellow powder, vitamin C phosphate 0.1125kg, peptidoglycan 0.125kg, allicin 0.025kg, Chinese mitten crab protein peptide 0.03kg.

[0028] The β-glucan, three yellow powder, vitamin C phosphate, peptidoglycan and allicin are powders with a particle size greater than 80 mesh.

[0029] The average molecular weight of the Chinese mitten crab protein peptide is 600-1500Da.

[0030] Accurately weigh the above-mentioned active ingredients according to the formula, use wheat secondary flour with a particle size greater than 80 mesh as a carrier, and mix according to the routine to prepare the non-specific immune preparation for cultivating Chinese mitten crabs of the present invention.

[0031] A method for using non-specific immune preparations for cul...

Embodiment 3

[0035] A non-specific immune preparation for cultivating Chinese mitten crabs, characterized in that each kilogram of the immune preparation contains the following active ingredients in weight: 0.075 kg of β-glucan, 0.1625 kg of three yellow powder, vitamin C phosphate 0.1125kg, peptidoglycan 0.125kg, allicin 0.025kg, Chinese mitten crab protein peptide 0.0225kg.

[0036] The β-glucan, three yellow powder, vitamin C phosphate, peptidoglycan and allicin are powders with a particle size greater than 80 mesh.

[0037] Accurately weigh the above-mentioned active ingredients according to the formula, use wheat secondary flour with a particle size greater than 80 mesh as a carrier, and mix according to the routine to prepare the non-specific immune preparation for cultivating Chinese mitten crabs of the present invention.

[0038] A method for using non-specific immune preparations for cultivating mitten crabs, characterized in that the specific implementation steps are as follows: ...

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Abstract

The invention relates to a non-specific immune preparation for culturing Chinese mitten crab. The non-specific immune preparation is characterized in that each kilogram of the immune preparation comprises the following active ingredients by weight: 0.02 kg to 0.2 kg of beta-glucan, 0.06 kg to 0.5 kg of Sanhuang powder, 0.05 kg to 0.3 kg of vitamin C phosphate, 0.05 kg to 0.3 kg of peptidoglycan and 0.01 kg to 0.1 kg of allicin. According to the invention, the immunity and the breeding survival rate of Chinese mitten crabs can be effectively improved.

Description

technical field [0001] The invention belongs to the technical field of crustacean breeding, in particular to a non-specific immune preparation for breeding Chinese mitten crabs and a use method thereof. Background technique [0002] Existing studies have shown that, like other invertebrates, crustaceans lack the specific immune function of vertebrates, and their immune response depends on non-specific immune mechanisms. Non-specific immunity, antioxidant capacity and body disease resistance are important indicators to measure the non-specific immunity of crustaceans. The non-specific immunity of Chinese mitten crab has been proved by many scholars, which provides the physiological basis and conditions for non-specific immune agents to play a role in Chinese mitten crab. The types of non-specific immune preparations include: polysaccharide preparations derived from microorganisms, Chinese herbal medicine preparations derived from animals and plants, nutritional factor anti-s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K20/163A23K10/30A23K20/174A23K20/147A23K20/105A01K61/59
CPCA23K50/80A23K20/163A23K10/30A23K20/174A23K20/147A23K20/105A01K61/59Y02A40/81Y02A40/818
Inventor 刘群王菁张丹夏苏东刘桐山王钢霍文慧冯守明
Owner 天津市动物疫病预防控制中心
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