An oxygen-carrying biomimetic nano antidote and its preparation method and application
A biomimetic nanotechnology, antidote technology, applied in the directions of antitoxin, nanotechnology, nanotechnology, etc., can solve the problems of wrong determination of the shape characteristics of poisonous snakes, strong equipment dependence, limited local effects, etc. Good in vitro stability and the effect of alleviating hypoxia
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Embodiment 1
[0050] Different proportions of red blood cell membranes, oleic acid oxyethylphosphorylcholine and liquid perfluorocarbons were prepared by the following construction method to prepare biomimetic nano-broad-spectrum antidote, and the particle diameters of nano-preparations prepared in various proportions were measured. Various ratios of erythrocyte membrane protein:OOPC:PFCs mass ratio are 1:5%:3, 1:10%:3, 1:20%:3, 1:40%:3, 1:5%:5.9, 1:10%: 5.9, 1:20%: 5.9, 1:40%: 5.9, 1:5%: 11.8, 1:10%: 11.8, 1:20%: 11.8, 1:40%: 11.8.
[0051] The construction method specifically includes the following steps:
[0052] (1) prepare the dehydrated alcohol solution that OOPC concentration is 10mg / mL; prepare the aqueous solution that red blood cell membrane concentration is 1.5mg / mL;
[0053] (2) two kinds of solutions in mixing step (1);
[0054] (3) Ultrasonicate the mixed solution probe in step (2) by ultrasonic breaker, ultrasonic for 3s, stop for 3s, and the duration is 3min;
[0055] (4)...
Embodiment 2
[0063] Place the successfully prepared biomimetic nano-broad-spectrum antidote in a PBS solution with a volume fraction of 10% serum and a pH of 7.4, and incubate it in a constant temperature incubator at 37°C for 48 hours, and incubate at 0h, 1h, 3h, 5h, and 7h. , 24h, 48h to measure its particle size distribution to evaluate its stability in the serum environment.
[0064] Such as figure 2 As shown, the laser particle size analysis shows that the obtained nanoparticles can keep the particle size basically unchanged within 2 days, the stability is good, and the particle size distribution is 200-250nm.
Embodiment 3
[0066] The oxygen content of the successfully prepared biomimetic nano broad-spectrum antidote was measured by a dissolved oxygen monitor.
[0067] Such as image 3 , in the process of preparing nano preparations, the oxygen content of the solution was up to 41.3 mg / L after 2 minutes of oxygenation, and the oxygen concentration increased by about 31 mg / L, indicating that the biomimetic nano broad-spectrum antidote has a strong oxygen-carrying capacity.
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