peg-plga encapsulated hemoglobin
A hemoglobin and encapsulation technology, applied in the field of blood substitutes, can solve problems such as safety and in vivo effectiveness that have not been reported, achieve good oxygen supply effect, solve vasoconstriction, and high stability.
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Embodiment 1
[0066] Embodiment 1, preparation PEG-PLGA encapsulated hemoglobin
[0067] The preparation of PEG-PLGA wrapped hemoglobin of the present invention comprises the following steps:
[0068] 1) Dissolve PEG-PLGA in ethyl acetate to make the concentration 20mg / mL (10-30mg / mL is acceptable), then mix 2mL PEG-PLGA solution with 0.4mL 120mg / mL (60-240mg / mL is acceptable) The aqueous solution of hemoglobin is mixed according to the mass ratio of 5:6 (5:4-5:12 is acceptable), and the ultrasonic cell disruptor is used to sonicate in an ice water bath at 80W (80-100W is acceptable) for 3min (3-5min is acceptable) , forming colostrum;
[0069] 2) Mix 10mL of surfactant aqueous solution, in this example, 7mL of 2wt% PVA aqueous solution and 3mL of 2wt% F68 aqueous solution (volume ratio 7:3, volume ratio 1:1-9:1 can also be used, and a single PVA Aqueous solution), add 10mL of the mixture of PVA aqueous solution and F68 aqueous solution to the colostrum obtained in step 1), mix, and use a...
Embodiment 2
[0071] Embodiment 2, the physicochemical property detection of PEG-PLGA encapsulated hemoglobin
[0072]The physical and chemical properties of the PEG-PLGA coated hemoglobin particles prepared in Example 1 were detected as follows:
[0073] 1. Observation of particles by transmission electron microscope
[0074] The particle morphology was observed with a transmission electron microscope (model JEM-200CX, purchased from JEOL Ltd.).
[0075] The result is as figure 1 As shown, the prepared PEG-PLGA-encapsulated hemoglobin is spherical particles, and these particles are uniformly dispersed without agglomeration. It is preliminarily confirmed that the prepared PEG-PLGA-encapsulated hemoglobin is a nanoparticle colloidal dispersion system with concentrated particle size distribution. Each PEG-PLGA coated hemoglobin particle shape and figure 1 The result was no difference.
[0076] 2. Determination of the particle size distribution of nanoparticles by dynamic light scattering ...
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