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Polypeptide hydrogel for promoting skin repair as well as preparation method and application thereof

A skin repair and collagen technology, applied in skin diseases, medical preparations containing active ingredients, peptides, etc., can solve the problem of not directly promoting wound regeneration

Active Publication Date: 2021-10-12
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The treatment of chronic wounds is mainly for wound management, but does not directly promote wound regeneration

Method used

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  • Polypeptide hydrogel for promoting skin repair as well as preparation method and application thereof
  • Polypeptide hydrogel for promoting skin repair as well as preparation method and application thereof
  • Polypeptide hydrogel for promoting skin repair as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The preparation of embodiment 1 KGH hydrogel of the present invention

[0052] According to the treatment needs of the present invention, according to the pre-test results (therapeutic effect), experimental animals (C57 mice) and application sites (mouse skin wounds) determine the treatment system as follows:

[0053]

[0054] 1) Self-assembled short peptide KGH (sequence: Ac-KLDLKLDLKLDLGGH-CONH 2， (as shown in SEQ ID No.1) was synthesized and purified by Shanghai Botai Biotechnology, the dry powder was dissolved in sterile water, and prepared as a 10mg / mL stock solution, and 30μL was added to the skin wound;

[0055] 2) Slowly add 10 μL of physiological saline to the wound surface to form a hydrogel.

Embodiment 2

[0056] Example 2 Distribution experiment of KGH hydrogel of the present invention on skin wound

[0057] 1) Experimental materials

[0058] Self-assembled short peptide KGH (sequence: Ac-KLDLKLDLKLDLGGH-CONH 2 )

[0059] Cy7 fluorescent dye (Solarbio)

[0060] 1.5mL EP tube (Thermo)

[0061] · 10μL, 200μL, 1mL pipette tips (Eppendorf)

[0062] · PBS solution (0.1M, pH=7.2)

[0063] ·NaHCO3 solution (1M)

[0064] ·C57 mice (8 weeks old)

[0065] Pentobarbital Sodium (Merck)

[0066] ·Isoflurane (RWD Life Science)

[0067] Equipment related to animal experiments (1mL syringe, razor, hair removal cream, iodophor, 8mm skin biopsy puncher, ophthalmic scissors, ophthalmic forceps, cotton swabs, gauze, surgical towels, etc.)

[0068] 2) Experimental method

[0069] Animal grouping: Cy7, Cy7-KGH two groups

[0070] Label KGH hydrogel according to Cy7 fluorescent dye instructions, and remove free fluorescent probes by ultrafiltration;

[0071] C57 mice were injected intrape...

Embodiment 3

[0080] Example 3 Experiment of KGH hydrogel of the present invention treating skin damage in diabetic mice

[0081] 1) Experimental materials

[0082] Self-assembled short peptide KGH (sequence: Ac-KLDLKLDLKLDLGGH-CONH 2 )

[0083] Streptozotocin (STZ, sigma)

[0084] ·Citrate buffer (0.1M, pH=4.5)

[0085] 1.5mL EP tube (Thermo)

[0086] · 10μL, 200μL, 1mL pipette tips (Eppendorf)

[0087] · PBS solution (0.1M, pH=7.2)

[0088] ·C57 mice (8 weeks old)

[0089] Pentobarbital Sodium (Merck)

[0090] Equipment related to animal experiments (1mL syringe, razor, hair removal cream, iodophor, 8mm skin biopsy puncher, ophthalmic scissors, ophthalmic forceps, cotton swabs, gauze, surgical towels, etc.)

[0091] 2) Experimental method

[0092] Animal grouping: DM(+NS), DM+KGH two groups

[0093] Mice were fasted for 12 hours before STZ injection, and could drink water freely, and each mouse was intraperitoneally injected with STZ at 150 mg / kg;

[0094] 3 days after the inje...

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Abstract

The invention relates to the field of pharmaceutical preparations, in particular to polypeptide hydrogel for promoting skin repair and a preparation method and application thereof. The invention provides a self-assembled peptide, and also provides a polypeptide hydrogel formed by an aqueous solution of the self-assembled peptide. The invention also provides a method for preparing the polypeptide hydrogel. The invention also provides an application of the polypeptide hydrogel in treatment of skin injury of diabetic mice. The polypeptide hydrogel can promote healing of injured skin of diabetic mice and generation of skin appendages, improve the positive rate of PCNA cells and promote accumulation of CD31 positive blood vessels and collagen. The invention also provides an application of the polypeptide hydrogel in in-vitro cell 3D culture, wherein the HaCaT cells of the human keratinocyte line grow spherically in the polypeptide hydrogel, and the expression levels of PDGF and VEGF of the cells are higher than those of two-dimensional cultured cells.

Description

technical field [0001] The invention relates to the field of pharmaceutical preparations, in particular to a polypeptide hydrogel for promoting skin repair and its preparation method and application. Background technique [0002] Chronic wound refers to the failure of the damaged skin and surrounding soft tissue to heal for more than 3 months. It is caused by the persistence and aggravation of the inflammatory process due to the specificity of the wound, such as decubitus / pressure sores and poor burn / scald healing; or as a complication of diseases such as diabetes, vascular insufficiency and immunodeficiency, such as diabetic foot and venous ulcers. Chronic trauma is associated with high morbidity and mortality due to the lack of effective treatments, and imposes a huge social / medical burden as well as physical and psychological / economic burden on patients. The normal wound healing process is divided into hemostatic, inflammatory, proliferative, and remodeling phases. Howe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C12N15/11A61K38/10A61K9/06A61P17/02
CPCC07K7/08A61K9/06A61K9/0017A61P17/02A61K38/00
Inventor 刘书云刘敬平楼鹏
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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