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Bacillus amyloliquefaciens strain LXBA.1 and application thereof

A technology of amylolytic spores and bacillus, applied in the field of microorganisms, can solve the problems of poor overall effect of disease prevention and growth promotion, unstable biocontrol effect, poor field colonization ability, etc., and achieve broad prospects for industrial application and stable antibacterial effect , Broad antibacterial spectrum effect

Active Publication Date: 2021-09-28
领先生物农业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the Bacillus amyloliquefaciens preparations on the market often have certain defects in the actual application process, such as single disease control, unstable biocontrol effect, poor field colonization ability, poor comprehensive effect of disease prevention and growth promotion, etc.; therefore , there is an urgent need to develop a multi-functional and high-efficiency bacterial agent with comprehensive functions, many types of disease prevention, and obvious growth-promoting effect

Method used

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  • Bacillus amyloliquefaciens strain LXBA.1 and application thereof
  • Bacillus amyloliquefaciens strain LXBA.1 and application thereof
  • Bacillus amyloliquefaciens strain LXBA.1 and application thereof

Examples

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Effect test

Embodiment 1

[0054] This embodiment provides a strain of Bacillus amyloliquefaciens LXBA.1, and its screening and identification methods are as follows:

[0055] 1. Targeted screening

[0056] The bacterial strain LXBA.1 in this example was isolated from the rhizosphere soil of healthy plants in the continuous cropping strawberry greenhouse in Changli, Hebei. The sampling and isolation methods are as follows:

[0057] Select a healthy growing plant, dig out the entire root system with a sampling shovel, tap the root system, discard the soil that is loosely combined with the root system, and brush off the soil that is tightly combined with the root system with a brush, which is the rhizosphere soil sample.

[0058] Weigh 10g of the rhizosphere soil sample and dissolve it in 90mL of sterile water, fully shake at 180r / min for 20min, take 1mL of the soil supernatant, transfer it to a selective medium with p-hydroxybenzoic acid as the only carbon source, and selectively cultivate The base comp...

Embodiment 2

[0070] The present embodiment provides the growth characteristics of Bacillus amyloliquefaciens bacterial strain LXBA.1, and test method is as follows:

[0071] 1. Optimum growth temperature

[0072] Prepare the seed solution (LB medium) of Bacillus amyloliquefaciens strain LXBA.1, inoculate it into a 250mL Erlenmeyer flask containing 50mL LB liquid medium according to the inoculation amount of 5%, and inoculate it at 4°C, 10°C, 20°C, and 30°C, respectively. , 40°C, 50°C, 60°C, 70°C in a constant temperature shaking incubator (rotating speed 180r / min) for 2 days, set 3 repetitions, observe and record the growth situation (whether it is turbid).

[0073] 2. Tolerance to acid and alkali

[0074] Prepare LB liquid medium with different pH values ​​(3.0, 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0), and inoculate 5% of Bacillus amyloliquefaciens strain LXBA.1 seed liquid (LB medium) respectively, at 30 Under the condition of ℃ and 180r / min, shake culture for 2 days, set 3 repetitions, ob...

Embodiment 3

[0086] The present embodiment provides a microbial agent, which is a fermentation product of Bacillus amyloliquefaciens strain LXBA.1; the preparation method is as follows:

[0087] 1. Preparation of liquid fermentation medium

[0088] Prepare the liquid fermentation medium according to the following composition: soybean flour 10g / L, glucose 10g / L, soluble starch 30g / L, peptone 5g / L, corn flour 15g / L, ammonium sulfate 1g / L, magnesium sulfate 0.5g / L, Potassium dihydrogen phosphate 0.3g / L, dipotassium hydrogen phosphate 0.3g / L, manganese sulfate 0.05g / L.

[0089] 2. Liquid fermentation

[0090] Inoculate the Bacillus amyloliquefaciens strain LXBA.1 in the NA solid medium and culture it at 34°C for 24 hours to obtain the Bacillus amyloliquefaciens solid flat plate seeds; transfer the primary seeds to the eggplant bottle containing the NA solid medium , cultivated at a temperature of 34°C for 36h to obtain the secondary seeds of Bacillus amyloliquefaciens solid plate; the secondar...

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Abstract

The invention provides a bacillus amyloliquefaciens strain LXBA.1 and application thereof. The preservation number of the bacillus amyloliquefaciens strain LXBA.1 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) NO.21868; the strain can effectively relieve the influence of autotoxic substances such as p-hydroxybenzoic acid on the growth vigor of plants, has relatively strong bacteriostatic activity on various germs such as strawberry root rot, ginger stem rot, potato scab and the like, has the growth promoting properties of dissolving silicon, dissolving phosphorus and potassium, fixing nitrogen, producing indoleacetic acid and siderophore and the like, is strong in colonization ability, has obvious effects of promoting the growth and development of root systems, preventing and controlling soil-borne diseases, increasing the crop yield, improving the crop quality and the like, and greatly reduce the use of chemical fertilizers and pesticides; and in addition, the strain also shows certain saline-alkaline tolerance activity and stronger bacteriophage resistance, is a safe, green and efficient multifunctional broad-spectrum disease-resistant growth-promoting strain, and has a wide industrial application prospect.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Bacillus amyloliquefaciens strain LXBA.1 and its application. Background technique [0002] Continuous cropping obstacle refers to the phenomenon that the same crop is continuously planted in the same field, and after the second growing season, the disease will aggravate, the growth will be stunted, and finally lead to a serious decline in quality and yield. The causes of continuous cropping obstacles are complex, mainly including soil compaction and secondary salinization, unbalanced distribution of soil nutrients, deterioration of soil micro-ecological system, aggravation of soil-borne diseases and insect pests, and plant autotoxicity. One of the main factors of continuous cropping obstacles has received more and more attention. Plant autotoxicity is a chemical substance produced by plants that can be toxic to plants of the same species or the same family. These chemi...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C09K17/14C09K101/00C12R1/07
CPCC12N1/20A01N63/22C09K17/14C09K2101/00Y02A40/10
Inventor 李丽艳孙杉杉葛振宇杨娜徐志文刘洪钧杜迎辉
Owner 领先生物农业股份有限公司
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