Collagen-silver nanoparticle composite gel, preparation method and application
A technology of silver nanoparticles and collagen, applied in the field of preparation, collagen-silver nanoparticle composite gel, can solve the problems of biological toxicity and safety hazards, cumbersome hydrogel steps, poor mechanical properties of the gel, etc., and achieve low cellularity Effects of toxicity, maintenance of triple helix structure and biological activity, and high mechanical strength
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Embodiment 1
[0041] Preparation and Characterization of Example 1 Collagen-Silver Nanoparticle Composite Gel
[0042] Collagen solution configuration: Dissolve the solid collagen (type I, II or III) with water or PBS buffer solution, configure it into a collagen solution, and dilute it with water or PBS buffer solution before use.
[0043] AgNO 3 Solution configuration: AgNO 3 The solid is dissolved in water and configured to a concentration of AgNO 3 Solution, diluted with water or PBS buffer solution immediately before use.
[0044] 1. Preparation of type I collagen-silver nanoparticles composite gel
[0045] Taking yak type I collagen as an example, the collagen-silver nanoparticle composite gel was prepared. The specific preparation process was as follows: at room temperature, collagen solution was added to a 12-well plate, and a visible light source (400nm3 Solution, reaction 2hrs. When preparing a sample for rheological performance testing, the composite material is made in a mol...
Embodiment 2
[0055] Example 2 Determination of rheological properties of collagen-silver nanoparticle composite gel
[0056] Prepare the type I collagen-silver nanoparticle composite gel of uniform thickness 1cm * 1cm * 1mm according to Example 1, then place it on the sample stage of the rheometer, fix the scanning frequency of the rheometer, and test the gel Storage modulus G'(Pa) and loss modulus G"(Pa) that vary with strain.
[0057] The results of type I collagen-silver nanoparticles composite gel are as follows: Image 6 As shown, the mechanical properties of the collagen solution without adding Ag ions are poor, only reaching 75Pa. With the increase of the concentration of silver ions, the storage modulus and loss modulus of the type I collagen-silver nanoparticle composite gel are obvious. increased. When the silver ion is 10mg / mL, the storage modulus of the type I collagen-silver nanoparticle composite gel reaches 666Pa; when the silver ion concentration is 25mg / mL, the storage m...
Embodiment 3
[0059] Example 3 Cell Adhesion of Collagen-Silver Nanoparticle Composite Gel
[0060] Take a 24-well plate that has not been treated with TC, put it into slides, add 10 mg / mL BSA solution, 0.01 mg / mL collagen gel, and 1 mg / mL type I collagen to the wells, and turn on the ultra-clean bench for ventilation. Material is allowed to air dry. Add 1 mL of PBS to each well to wash away impurities on the sample surface. Digest the well-growing HFF-1 cells with 1 mL of trypsin, shake them left and right for about 1 minute, and observe the cell shape under a microscope. If the cell shape becomes round and a small amount of cells swim, add 1 mL of FBS to stop the digestion, and use a pipette to stop the digestion. Aspirate the solution, add 2 mL of DMEM solution to blow it down from the bottom of the bottle, and dilute with DMEM to form a cell suspension. Use a hemocytometer to count the number of cells, and add 1 mL of cell suspension in a 96-well plate at 100,000 cells per well in 5% ...
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