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Preparation method of corn alcohol-soluble protein nanofiber membrane used for cell culture

A technology of zein and nanofiber membranes, which is applied in tissue cell/virus culture devices, biochemical equipment and methods, fiber treatment, etc., can solve the problem of poor mechanical properties, difficult removal of chemical reagents, and effects on zein Fiber biocompatibility and other issues, to achieve the effect of improved mechanical strength, simple and effective preparation method, and good stability

Inactive Publication Date: 2012-07-18
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has disadvantages such as poor mechanical properties and needs to be improved.
[0005] Since it is difficult to completely remove the residual chemical reagents after chemical modification, the residual toxicity affects the biocompatibility of zein fiber

Method used

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  • Preparation method of corn alcohol-soluble protein nanofiber membrane used for cell culture
  • Preparation method of corn alcohol-soluble protein nanofiber membrane used for cell culture
  • Preparation method of corn alcohol-soluble protein nanofiber membrane used for cell culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Dissolve 1.5-4.0 grams of zein in a mixed solvent of 10 ml ethanol and water, the volume ratio of ethanol and water is 80:20, 70:30, 60:40 to form 15%-40% (w / v) Then add it into a plastic syringe, the syringe is connected to a high-voltage positive electric field, the distance between the nozzle and the receiver is controlled at 15cm-25cm, the solution flow rate is 0.5mL / h-2mL / h, the voltage is 10kV-18kV, and electrospinning is carried out. , the nanofibers with ribbon structure were obtained, and the nanofibers stacked randomly into a zein nanofiber film.

Embodiment 2

[0026] Dissolve zein in the mixed solvent of 10ml ethanol and water, the volume ratio of ethanol and water is 70:30, then add polyvinyl alcohol, the mass ratio of zein and polyvinyl alcohol is 5:1,4 : 1, 3: 1, 2: 1, 1: 1, magnetically stirred at 80°C for 12 hours, made into a polymer solution with a mass concentration of 15%, and then added to a plastic syringe, the syringe was connected to a high-voltage positive electric field, and the injection head and the receiver The distance between objects is controlled at 15cm-25cm, the solution flow rate is 0.5mL / h-2mL / h, and the voltage is 0kV-18kV. Electrospinning is performed to obtain a nanofiber membrane with greatly improved mechanical properties.

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Abstract

The invention discloses a preparation method of a corn alcohol-soluble protein nanofiber membrane used for cell culture. The corn alcohol-soluble protein / polyvinyl alcohol nanofiber membrane is a cell culture carrier material, which comprises biodegradable natural polymer corn alcohol-soluble protein and polyvinyl alcohol as a biocompatible high polymer. The transverse dimension of a ribbon-like corn alcohol-soluble protein / polyvinyl alcohol nanofiber is 625 to 3,920 nanometers; and the nanofibers are stacked into the nanofiber membrane randomly. The corn alcohol-soluble protein / polyvinyl alcohol nanofiber membrane is made through electrostatic spinning process, the preparation method is simple and effective, preparation equipment is simple, the stability is high, and indexes such as the diameters of the nanofibers and the porosity of a membrane material and the like can be regulated through changes of the condition of the electrostatic spinning process. The experiment proves that compared with a common PC (polycarbonate) plate, the corn alcohol-soluble protein nanofiber membrane material contributes to proliferation of mouse embryonic stem cells, and the diameter of the stem cell clone cultured for 5 days reaches 100 micrometers. Moreover, the nanofiber membrane can be recycled after being steamed and pressed for 30 minutes at 120 DEG C.

Description

technical field [0001] The invention relates to a method for preparing a nanofibrous membrane that can be used for cell culture. The biodegradable nanofiber can be used as a support material for cell culture, and belongs to the technical field of cell culture carrier materials. Background technique [0002] In tissue engineering, cell carrier materials play the role of extracellular matrix, which is a bionic of the structure and function of extracellular matrix. The similarity in morphology and structure between the nanofibrous scaffold prepared by electrospinning and the extracellular matrix provides a source of motivation for its application in tissue engineering. The nanofibrous support material has high surface area and porosity, which facilitates cell adhesion and entry into the membrane material. [0003] Zein is the main protein in corn gluten powder, which has good biocompatibility, biodegradability, film-forming properties and other properties. As a class of biode...

Claims

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Application Information

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IPC IPC(8): D04H1/4382D04H1/728D01D5/00C12M3/00
Inventor 张梅孙大辉刘永佳张雁蔡红莉
Owner JILIN UNIV
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